RESUMEN
BACKGROUND: Oral leukoplakia (OLK) and oral lichen planus (OLP) are common precancerous lesions of the oral mucosa. The role of circular RNAs (circRNAs) in OLK and OLP is unclear. The aim of this study was to evaluate the circRNA expression profiles of OLK and OLP, and further explore the potential role of circRNAs in the pathogenesis of these two diseases. METHODS: High throughput sequencing technology was performed to detect the differentially expressed circRNA in OLK (n = 6), OLP (n = 6), oral squamous cell carcinoma (n = 6), and normal oral mucosa tissues (n = 6). Expression of selected circRNAs was validated by qRT-PCR, enzyme tolerance assay, and Sanger sequencing. Expanded sample size validation was done in 20 tissue pairs. The biological processes and signal pathways involved in differential circRNA were analyzed by GO and KEGG enrichment. TargetScan and MiRanda were used to predict miRNAs downstream of circRNA and draw competitive endogenous RNA network diagram. RESULTS: Forty-nine circRNAs were significantly altered in OLK and OLP, including 30 upregulated and 19 downregulated circRNAs. The five selected circRNAs were validated by qRT-PCR, Sanger sequencing, and RNase R assay. GO and KEGG analyses indicated that the upregulated circHLA-C may be involved in the biological process of immune function of OLK and OLP. Bioinformatics analysis indicated that circHLA-C may be involved in the progression of OLK and OLP as a ceRNA. In validation with expanded sample size, PCR results showed that circHLA-C expression was significantly upregulated in OLK and OLP. ROC analysis indicated that circHLA-C has potential diagnostic value with good accuracy and specificity. CONCLUSION: Our study revealed that circHLA-C is the most significantly upregulated circRNA co-existing in OLK and OLP, and we preliminarily discuss the role of circHLA-C in the etiopathogenesis and progression of OLK and OLP.
Asunto(s)
Carcinoma de Células Escamosas , Liquen Plano Oral , MicroARNs , Neoplasias de la Boca , Humanos , ARN Circular/genética , ARN Circular/metabolismo , Neoplasias de la Boca/genética , Neoplasias de la Boca/patología , Carcinoma de Células Escamosas/patología , Liquen Plano Oral/genética , Liquen Plano Oral/patología , Leucoplasia Bucal/genética , Leucoplasia Bucal/patologíaRESUMEN
Objective To discuss the effect of deep pharyngeal muscle nerve stimulation combined with Xingnao-Kaiqiao acupuncture on patients with Pharyngeal dysphagia after stroke . Methods According to the digital table method, 99 patients with Pharyngeal dysphagia after stroke were randomly divided into A group, B group and C group, 33 cases in each group. A group received deep pharyngeal muscle nerve stimulation combined with Xingnao-Kaiqiao acupuncture; B group received deep pharyngeal nerve stimulation; and C group received Xingnao-Kaiqiao acupuncture treatment. The conventional treatment were used in all groups After 4 weeks of treatment, the relevant evaluation indexes were compared before and after treatment. Results After treatment, the Grade of aspiration (4.2 ± 0.5, 4.0 ± 0.4 vs. 3.0 ± 0.5, F=6.117) was significantly higher than A group (P<0.05), dysphagia score (5.3 ± 1.3, 5.1 ± 1.1 vs. 7.6 ± 1.4, F=6.541) in B and C groups was significantly lower than A group (P<0.05). The total effective rate [96.4% (27/28), 85.7% (24/28) vs. 82.1% (23/28), χ2=4.766, 7.791] of A group was significantly higher than that of B group and C group (P<0.05). Conclusions The deep pharyngeal nerve stimulation combined with Xingnao-Kaiqiao acupuncture could treatthe patients with dysphagia after stroke.