Responses of some matrix metalloproteinases activities to an acute session of endurance exercise and electrical stimulation in induced myocardial infarction in Wistar rats.

OBJECTIVE: Myocardial infarction is the irreversible cell death of cardiac muscle that takes place after the blood flow is cut off to a specific region of the heart muscle. The molecular angiogenesis process that may follow after the incidence, due to any activity or its intensity, is unknown. The purpose of this research was to examine some of the matrix metalloproteinase (MMP) responses to an acute course of endurance exercise and electrical stimulation in induced myocardial infarcted Wistar rats. MATERIALS AND METHODS: In this experimental case-control study, 40 induced myocardial infarcted Wistar rats (8-week-old, mean weight 130±30 g) were randomly assigned into 4 conditions: endurance exercise, exercise + electrical stimulation, only electrical stimulation, and control group. The infarction was induced 24 hours after the subcutaneous injection of 150 mg/kg of Isoproterenol. The exercise and exercise plus electrical stimulation groups performed a session of endurance exercise on an animal treadmill, at 20 m/min for one hour. The electrical stimulation was delivered by foot shock, set with the intensities of 0.5 mA for 20 minutes. Immediately after the cessation of the treatment protocol, MMP1, MMP2, and MMP9 were measured by the ELISA method. Data analysis was performed by using Two-way ANOVA and significance was set at α = 0.05. RESULTS: One session of endurance exercise or electric stimulation, or their combination, had no significant effect on the level of MMPs. CONCLUSIONS: One session of acute endurance exercise, stimulation, or their combination, elicited no significant effect on the level of MMPs of artificially induced myocardial infarcted Wistar rats.

The effects of game types on intensity of small-sided games among pre-adolescent youth football players.

The aim of this study was to examine the effects of variations in pitch dimensions on pre-adolescent youth soccer players' physiological responses during two different types of small-sided games (SSG). Sixteen young soccer players (age: 13.2 ± 0.6 years; body mass: 52.5 ± 7 kg; height: 163.4 ± 6 cm) participated in this study. They performed 4 vs. 4 stop-ball SSG (SB-SSG) vs. small-goals SSG (SG-SSG) with 4×4 min and 2 min of passive recovery in between, using 3 different pitch sizes (small: 10×15, medium: 15×20, and large: 20×25 m). Heart rate (HR), lactate concentration ([La-]), and rating of perceived exertion (RPE) were measured during each session. The results show that SB-SSG induced higher HR responses than SG-SSG for the 3 pitch sizes: for HR (167.2±3.0 vs. 164.5±3.0, 172.3±2.9 vs. 169.2±3.1, and 175.4±3.1 vs. 171.1±2.7 bpm; P<0.05, for small, medium, and large, respectively) and [La-] (7.1±1.0 vs. 6.5±1.04, 7.3±1.0 vs. 6.8±1.2, and 7.8±0.9 vs. 7.1±0.8 mmol·l-1; P<0.05 on small, medium, and large pitches, respectively), whereas RPE scores were significantly higher during SB-SSG compared to SG-SSG (6.2±1.0 vs. 5.8±0.9; P<0.05, respectively) on the small pitch. In the present study higher physiological responses were observed in SSG in pre-adolescent young soccer players when using the stop-ball conditions in comparison with the small-goal rule for all pitch sizes - small, medium, and large. Stop-ball conditions in comparison with the small-goal rule for all pitch sizes - small, medium, and large.

Soccer small-sided games in young players: rule modification to induce higher physiological responses.

The aim of this study was to identify the physiological responses of 3 forms of players' numbers during two different games rules of small-sided games (SSG: stop-ball vs. small-goals rules). Eighteen youth amateur soccer players (age 13.5±0.7 years; height 168.9±6.1cm; body mass 63.1±7.7 kg) participated in this study and performed 3 SSGs with varying players' number (2vs.2; 3vs.3 and 4vs.4): stop-ball SSG (SB-SSG) vs. small-goals SSG (SG-SSG) in a randomized and counter-balanced order on a constant pitch dimension (20×25m). The players performed 4×4 min SSG with 2-min of passive recovery in-between. Heart rate (HR), (expressed in bpm and % HRmax), lactate ([La-]), and rating of perceived exertion (RPE) were collected during each session. SB-SSG induced the higher HR values in comparison with the SG-SSG for the 3 game formats (2vs.2; 3vs.3 and 4vs.4). Also, compared with SG-SSG, SB-SSG induced the higher HR values during 2vs.2 compared with 4vs.4 games rules (178 vs. 174 and 175 vs. 171 bpm, respectively). However, the SB-SSG was more intense compared with SG-SSG in the 2 vs. 2 game rule compared with the two others (3 vs.3 and 4 vs. 4) for [La-] and RPE (7.58 vs. 7; 7.25 vs. 6.75 and 6.5 vs. 6.16 mmol ∙ L-1, and 7.75 vs. 7.33; 7.41 vs. 7.08 and 7.16 vs. 6.83, respectively). Therefore, the use of 2 vs. 2 and 3 vs. 3 SSG with SB-SSG seems to represent an alternative to coaches to increase cardiovascular and metabolic demands in youth soccer players.