RESUMEN
The aim of this study is to prepare an optimized surface active modified polysaccharide gel of oleoyl alginate using response surface methodology as well as to study its potential use in drug skin delivery as a safe alternative to skin penetration enhancers. An optimized oleoyl alginate material was obtained using response surface methodology in terms of three different responses, i.e. degree of substitution, viscosity and surface tension. The resultant optimized oleoyl alginates were further studied concerning particle size, surface roughness and particle aggregation concentration measurements. Franz diffusion cell method was used to monitor skin permeation of ibuprofen lysinate over 6-h period exposure to excised rabbit skin. The optimized oleoyl alginate based on surface tension showed the highest particle size, the lowest surface tension among other preparations and it has also been used as skin penetration enhancer for ibuprofen lysinate. In this study, a polysaccharide derivative was prepared that enhanced skin penetration ability of the model drug i.e. ibuprofen lysinate.
Asunto(s)
Alginatos/química , Portadores de Fármacos/química , Diseño de Fármacos , Ácidos Oléicos/química , Polisacáridos/química , Piel/efectos de los fármacos , Administración Cutánea , Alginatos/administración & dosificación , Animales , Portadores de Fármacos/administración & dosificación , Geles , Ibuprofeno/administración & dosificación , Ibuprofeno/análogos & derivados , Técnicas In Vitro , Lisina/administración & dosificación , Lisina/análogos & derivados , Ácidos Oléicos/administración & dosificación , Tamaño de la Partícula , Polisacáridos/administración & dosificación , Conejos , Piel/metabolismo , Absorción Cutánea , Solubilidad , Espectroscopía Infrarroja por Transformada de Fourier , Propiedades de Superficie , Tensión Superficial , ViscosidadRESUMEN
The present work investigated the possibility of using mesoporous silica nanoparticles coated with low molecular weight chitosan as an injectable controlled release carrier of insulin. Insulin was totally encapsulated in particles. Surface tension measurements indicated that insulin was absorbed into mesoporous silica pores and interacted with chitosan. The stability of the encapsulated insulin was confirmed by different analytical methods such as RP-HPLC, FTIR and CD. Furthermore, the thermal stability using DSC was in the favor of the encapsulated insulin compared to free insulin. In vivo results indicated that insulin release was prolonged after loading into mesoporous silica nanoparticles. Such particles could be a potential carrier to control the release of insulin.