Impact of silver ions and silver nanoparticles on biochemical parameters and antioxidant enzyme modulations in Saccharomyces cerevisiae under co-exposure to static magnetic field: a comparative investigation.

The increase in simultaneous exposure to magnetic fields and other hazardous compounds released from industrial applications poses multiple stress conditions on the ecosystems and public human health. In this work, we investigated the effects of co-exposure to a static magnetic field (SMF) and silver ions (AgNO3) on biochemical parameters and antioxidant enzyme activities in the yeast Saccharomyces cerevisiae. Sub-chronic exposure to AgNO3 (0.5 mM) for 9 h resulted in a significant decrease in antioxidant enzyme activity, including glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and glutathione transferase (GST). The total glutathione (GSH) level increased in yeast cells exposed to Ag. Additionally, a notable elevation in malondialdehyde (MDA) levels and protein carbonyl content was observed in both the AgNP and AgNO3 groups compared to the control group. Interestingly, the SMF alleviated the oxidative stress induced by silver nitrate, normalizing antioxidant enzyme activities by reducing cellular ROS formation, MDA levels, and protein carbonylation (PCO) concentrations.

Musico-bioremediation of seafood canning wastewater by Yarrowia lipolytica.

Due to the lack of water resources and the harmful effects of wastewater on environment and human health, treatment of wastewater becomes necessary. The present study explored the effect of musical sounds on the biological treatment of seafood canning wastewater by using Yarrowia lipolytica. Our results showed that low frequency (21 Hz to 1356 Hz) and high frequency (21 Hz to 16,214 Hz) musical sounds stimulated the growth of Y. lipolytica and increased the polluant removal efficiency. Such treatment decreased significantly the chemical oxygen demand (COD) and salinity as well as the color of this wastewater. Our study revealed that low frequency musical sounds are more effective in COD (87.5%) and salinity (44%) reduction as well as the decolorization (86.46%) of this effluent. Additionally, after 7 days of incubation significant yeast cell dry biomass (3.46 ± 0.22 g/L) and single cell proteins (46.45 ± 0.7 mg/g) were obtained under low frequency waves. Musico-bioremediation represents an innovative ecotechnological approach to wastewater treatment with low operating costs and significant environmental benefits.

Nanotechnology in drug and gene delivery.

Over the last decade, nanotechnology has widely addressed many nanomaterials in the biomedical area with an opportunity to achieve better-targeted delivery, effective treatment, and an improved safety profile. Nanocarriers have the potential property to protect the active molecule during drug delivery. Depending on the employing nanosystem, the delivery of drugs and genes has enhanced the bioavailability of the molecule at the disease site and exercised an excellent control of the molecule release. Herein, the chapter discusses various advanced nanomaterials designed to develop better nanocarrier systems used to face different diseases such as cancer, heart failure, and malaria. Furthermore, we demonstrate the great attention to the promising role of nanocarriers in ease diagnostic and biodistribution for successful clinical cancer therapy.

Novel static magnetic field effects on green chemistry biosynthesis of silver nanoparticles in Saccharomyces cerevisiae.

The bacteriocidal properties of silver nanoparticles (AgNPs) depend on their average diameter (toxicity increases with decreasing diameter). In the present work, we describe novel green chemistry biosynthesis of AgNPs from AgNO3 added to cell-free culture medium of baker's yeast, Saccharomyces cerevisiae, yielding nanoparticles in the range 11-25 nm. However, when yeast was grown in a moderate static magnetic field, AgNPs obtained from the resulting cell-free culture medium, were significantly smaller (2-12 nm) than those obtained without magnetic field. These latter nanoparticles were highly crystalline, stable and near-uniform shape. Furthermore, the antibacterial activity of AgNPs obtained from static magnetic fields were greater than those from control cultures. Static magnetic fields show a promising ability to generate biocidal nanoparticles via this novel green chemistry approach.

High-performance biological treatment of tuna wash processing wastewater using Yarrowia lipolytica.

It is well known that the lack of an effective treatment of tuna wash processing wastewater may pose substantial environmental and public health hazards. The present work investigates the performance of biological treatment of tuna wash processing wastewater (TWPW) by using Yarrowia lipolytica. Under optimized experimental conditions (pH "6.40-6.50" and 29 °C), Y. lipolytica reduced the pollution level of the crude and the diluted TWPW after only 7 days of incubation. The Yarrowia treatment leaded to a reduction of 66% chemical oxygen demand, 69.8% total organic carbon, 66% salinity, and phosphorus total (100%) removal of the crude TWPW, while the treated-diluted TWPW revealed significant reductions in chemical oxygen demand and total organic carbon (75% and 74%, respectively), as well as salinity (68%). Interestingly, a total removal of nitrogen and phosphorus from the diluted TWPW was obtained. Under high salinity, an important Y. lipolytica biomass of 5 g L-1 is produced with high levels of lipids and protein contents at around 336 ± 12.2 mg g-1 and 302.15 ± 5.44 mg g-1, respectively. The phytotoxicity assessment of the treated TWPW on fenugreek seeds shows promising results, which reveals the good performance of Yarrowia treatment in reducing the toxicity of this wastewater.

Synthesis, Antimicrobial Activity and Molecular Docking Study of Novel α-(Diphenylphosphoryl)- and α-(Diphenylphosphorothioyl)cycloalkanone Oximes.

A series of novel α-(diphenylphosphoryl)- and α-(diphenylphosphorothioyl)cycloalkanone oximes have been synthesized in search for novel bioactive molecules. Their structures were characterized by various spectroscopic methods including IR, NMR (1 H, 31 P, 13 C), mass spectrometry and single crystal X-ray diffraction. The newly synthesized phosphorus-containing oximes were screened for their in vitro antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), Gram-negative bacteria (Escherichia coli and Salmonella typhimurium) and fungal strains (Candida albicans and Candida glabrata). The biological assays showed that all the studied compounds exhibited high antibacterial and antifungal activities at only 0.1-2.1 µg/mL. In silico molecular docking studies in FabH enzyme active site were performed in order to predict the possible interaction modes and binding energies of the drug candidates at the molecular level.

Alleviated Actions of Plantago albicans Extract on Lead Acetate-Produced Hepatic Damage in Rats Through Antioxidant and Free Radical Scavenging Capacities.

The aim of this study was to explore the possible protective mechanisms and to determine the antioxidant capacity of phenolic compounds extracted from Plantago albicans against lead acetate-induced hepatic injury. High performance liquid chromatography-photo diode array/electrospray ionization-mass spectrometry (HPLC-PDA/ESI-MS) assay was used to identify the P. albicans extract phenolic compounds. Animals received 100 mg of lead acetate/kg of body weight (bw) in the drinking water for a period of 30 days. The other groups of rats were orally administered with silymarin (300 mg/kg bw) or the P. albicans extract at two doses (100 and 300 mg/kg of bw), once daily, by gastric gavage for the same time. The P. albicans exhibited high total phenolic, flavonoid, and anthocyanin contents. The antioxidant in vitro activity demonstrated that the P. albicans exhibits an important effect against deleterious reactive species. The in vivo results showed that P. albicans prevented the lead acetate-induced significant changes on serum and liver lipid levels. In contrast, P. albicans succeeded in improving the biochemical parameters of serum and liver bringing them closer to the normal values of the control group. It also significantly promoted (P < .05) pro-inflammatory cytokines (TNF-α, IL-6, and NF-κB) in the liver of the experimental animals. The evaluated sample with HPLC-PDA/ESI-MS method showed to contain 10 dominant polyphenols, 2 hydroxycinnamic acids (p-coumaric acid and chlorogenic acids), 4 flavones (Apigenin, Luteolin, Cirsiliol, and Luteolin-7-O-rutinoside), and an anthocyanin (cyanidin-3-glucoside). Hence, it can be concluded that P. albicans could be a potent source of health-beneficial phytochemicals providing a novel therapy to protect liver against lead exposure.

Dual Valorization of Olive Mill Wastewater by Bio-Nanosynthesis of Magnesium Oxide and Yarrowia lipolytica Biomass Production.

This research investigates an efficient dual valorization of olive mill wastewater in the biosynthesis of magnesium oxide nanoparticles and in the depollution of the effluent by Yarrowia lipolytica growth evaluation. After removal of polyphenols, the recovered biophenols were reacted with the magnesium precursor to provide magnesium oxide nanoparticles. In order to confirm the biosynthesized magnesium oxide nanoparticles, several analyses were undertaken. The Fourier transform infrared spectrum gives a broad absorption at 658 cm-1 confirming the presence of the magnesium oxide nanoparticles, while the UV/VIS absorption spectroscopy reveals an intense transition with a maximum absorption at 300 nm. The X-ray diffraction and transmission electron microscopy analyses show that nanoparticles are in pure cubic crystalline with spherical and hexagonal shapes (average size is 19.4 nm). The zeta potential analysis illustrates a negative potential proving a good stability of the biosynthesized nanoparticles. Nanoparticles were assigned for their in vitro antibacterial activity against Escherichia coli, Enterobacter aerogenes, Salmonella typhimurium, Staphylococcus cohnii, and Bacillus niacini. The evaluation of the growth of Yarrowia lipolytica on the recovered olive mill wastewater after removal of polyphenols yielded 3.2 g/L of the Yarrowia biomass in 72 h without nutriment additions, providing an important decrease of chemical oxygen demand (73 %).