RESUMEN
Two Gram-stain-negative, aerobic, non-motile and short-rod-shaped bacteria, designated as strains GL-53T and GL-15-2-5, were isolated from the seamount area of the West Pacific Ocean and identified using a polyphasic taxonomic approach. The growth of strains GL-53áµ and GL-15-2-5 occurred at pH 5.5-10.0, 4-40â°C (optimum at 28â°C) and 0-10.0â% NaCl concentrations (optimum at 0-5.0â%). On the basis of 16S rRNA gene sequence analysis, strains GL-53áµ and GL-15-2-5 exhibited the highest similarity to Rheinheimera lutimaris YQF-2T (98.4â%), followed by Rheinheimera pacifica KMM 1406T (98.1â%), Rheinheimera nanhaiensis E407-8T (97.4â%), Rheinheimera aestuarii H29T (97.4â%), Rheinheimera hassiensis E48T (97.2â%) and Rheinheimera aquimaris SW-353T (97.2â%). Phylogenetic analysis revealed that the isolates were affiliated with the genus Rheinheimera and represented an independent lineage. The major fatty acids were summed feature 3 (C16â:â1 ω7c and/or C16â:â1 ω6c), C16â:â0 and summed feature 8 (C18â:â1 ω7c and/or C18â:â1 ω6c). The sole isoprenoid quinone was ubiquinone 8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid (and one unidentified glycolipid. The DNA G+C content was 48.5âmol%. The average nucleotide identity, average amino acid identity and in silico DNA-DNA hybridization values among the genomes of strain GL-53áµ and the related strains in the genus Rheinheimera were 75.5-90.1â%, 67.5-93.9â% and 21.4-41.4â%, respectively. Based on their phenotypic, chemotaxonomic and genotypic properties, the two strains were identified as representing a novel species of the genus Rheinheimera, for which the name Rheinheimera oceanensis sp. nov. is proposed. The type strain is GL-53T (=KCTC 82651T=MCCC M20598T).
