RESUMEN
Urtica L. has been long used for gout in traditional Tibetan medicine and is closely related to the effect of reducing uric acid. This study aimed to investigate the effect of Urtica hyperborea Jacq. ex Wedd. (UW) on lowering uric acid and its mechanism by using HK2 cells and hyperuricemia mouse model. Petroleum ether extract (UWP), ethyl acetate extract (UWE), n-butanol extract (UWB), and alcohol-soluble extract (UWA) from UW were prepared, and HK2 cells were treated with various parts extracts to observe the expression of uric acid transporter at 25, 50, and 100 µg/mL for 24 h. Moreover, hyperuricemia mice were administered orally various parts extracts at 0.78 and 2.34 g/kg (crude drug dose converted by extraction rate) to observe the change of hepatic XOD, serum ADA, renal function, and uric acid transporter. In vitro experiments showed that UWA can remarkably elevate OAT1 expression and decrease URAT1 expression in HK2 cells. In vivo experiments showed that UWP, UWE, UWB, and UWA showed remarkable activity in reducing uric acid, rendering a substantial decline in the SUA level in hyperuricemia mice. Compared with the hyperuricemia and allopurinol groups, UWB and UWA had significant protective effects on renal injury. At the same time, UWA can significantly reduce the activity of XOD and ADA, reduce the expression of URAT1, and increase the expression of OAT1. These results indicated that UWA had an outstanding uric acid lowering effect and did not affect renal function. This may be related to increased uric acid excretion and decreased uric acid production, mediated by renal OAT1, URAT1, liver XOD, and serum ADA. UWA may be a potential drug against hyperuricemia.
Asunto(s)
Antígenos de Neoplasias/metabolismo , Hiperuricemia/tratamiento farmacológico , Riñón/efectos de los fármacos , Riñón/metabolismo , Proteína 1 de Transporte de Anión Orgánico/metabolismo , Transportadores de Anión Orgánico/metabolismo , Proteínas de Transporte de Catión Orgánico/metabolismo , Extractos Vegetales/farmacología , Ácido Úrico/farmacología , Urticaceae/química , Animales , Línea Celular , Modelos Animales de Enfermedad , Humanos , Hiperuricemia/metabolismo , Masculino , RatonesRESUMEN
A new 6,9-cyclotetrahydrofuranlignan, 3,3'-dimethoxy-4,4'-dihydroxy-8ß,9ß,8'α-6,9-cyclotetrahydrofuranlignan, was isolated from the stem of Syringa pinnatifolia Hems1.var. alashanensis MA. et S. Q. ZHOU. Its structures were elucidated on the basis of spectroscopic methods including UV, IR, ESI-MS, 1D NMR and 2D NMR.
Asunto(s)
Lignanos/química , Tallos de la Planta/química , Syringa/química , China , Furanos/química , Furanos/aislamiento & purificación , Lignanos/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Estructura MolecularRESUMEN
A new lignan, 4,4'-dihydroxy-3,3'-dimethoxy-6,9'-cycloligna-7,8'-dien-9'-al, was isolated from the stem of Syringa pinnatifolia Hems1. var. alashanensis MA. et S.Q. Zhou (S. pinnatifolia). This is the first report on the structure elucidation of a new compound based on spectroscopic methods including UV, IR, ESI-MS, 1D NMR and 2D NMR techniques.
Asunto(s)
Naftoles/química , Syringa , Lignanos/química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Extractos Vegetales/química , Tallos de la Planta/química , Espectrometría de Masa por Ionización de Electrospray , Espectrofotometría Infrarroja , Espectrofotometría Ultravioleta , Syringa/químicaRESUMEN
We report the isolation of a new sesquiterpene, named as syripinol (1), and two known compounds from the volatile oil of the stems of Syringa pinnatifolia, for the first time. The structures of the isolated compounds were elucidated on the basis of spectroscopic methods including UV, IR, ESI-MS, 1D and 2D NMR techniques. The antibacterial activities of the compounds were also evaluated against various bacteria which showed good results.