[Detection and Characteristics of Hepatitis C Virus Infection among Blood Donors in Qingyang City].

OBJECTIVE: To deeply understand the epidemic status of hepatitis C virus (HCV) among blood donors in Qingyang City, and prevent the risk of transmission of HCV via blood. METHODS: To compare the application of nucleic acid and enzyme immunoassay adopted in HCV screening of volunteer blood donors in Qingyang City. The characteristics of positive population and the status of co-infection were studied. RESULTS: From 2015 to 2020, the positive rate of HCV detection among blood donors in Qingyang City was 3.2‰. Among the HCV positive co-infections, treponema pallidum (TP) accounted for the largest proportion, followed by alanine aminotransferase (ALT) and HBsAg. Blood donation was mainly based on individual voluntariness, and the main age group was 26-45 years old. The distribution of blood type showed that 31.08% for type B, 28.31% for type O, 27.38% for type A and 13.23% for type AB, the population of type AB was lower than that of type B and type O. The distribution of sex showed that male was more than female. The marital status was concentrated in married population. The regional distribution was concentrated in Xifeng District. Qingcheng County was higher than Huachi County and other places, while Ning County, Heshui County, Zhenyuan County and Huan County were close. The occupation was dominated by unemployed. Their nationalities were all Han. The overall awareness rate of HCV knowledge in young volunteers was 67.2%. CONCLUSION: There are more HCV infected people with TP infection in voluntary blood donors, and the cognitive level of HCV knowledge in young volunteers needs to be improved.

[Screening of HBV and Positive Population Distribution Characteristics of Voluntary Blood Donors in Qingyang].

OBJECTIVE: To study and analyse the application of ELISA and nucleic acid test (NAT) to HBV detection of voluntary blood donors in Qingyang city and the characteristics and social attributes of HBV positive population. METHODS: The HBV of 70 659 blood donors in Qingyang city from January 2013 to December 2016 was detected by using ELISA and NAT, and the statistical analysis of results was performed by means of X2 detection and FIsher' s Exact test. RESULTS: The HBV positive rate detected by ELISA was 0.47% in 70 659 blood samples, and the HBV positive rate detected by NAT was 0.05% in 47 913 HBV negative samples detected by ELISA, moreover, the number of HBV negative by ELISA detection yet HBV positive by NAT detection increased year by year. The statistical analysis of positive population characteristics showed that the age distoibution was mainly between 26-45 years old, however the difference of detected rate between 26-45 years old and 18-25 years old gradually decreased on near 2 years, showing that the positive population tended to be younger. The distribution of blood type was B>O>A>AB, while the sex distribution was male> female, but this difference showed gradually nairowing trend due to increment of female voluntary donors with HBV positive; for marital status, HBV positive concentrated in married population. The regional distribution of HBV positive mainly concentrated in Xifeng region, mainly in jobless population; the HBV positive comparison betwwen anationalities showed Han natienatity was > minoring nationalitie. CONCLUSION: The application of ELISA combined with NAT for the HBV screaning of blood voluntary donors in Qiangyang city can prevent the detection failed and occult HBV, and reduce the potential risk of blood transfution. The characteristis analysis of HBV positive population provides practical basis for the safe use and transfution of blood.

Genome-Wide Identification, Classification, and Expression Analysis of Amino Acid Transporter Gene Family in Glycine Max.

Amino acid transporters (AATs) play important roles in transporting amino acid across cellular membranes and are essential for plant growth and development. To date, the AAT gene family in soybean (Glycine max L.) has not been characterized. In this study, we identified 189 AAT genes from the entire soybean genomic sequence, and classified them into 12 distinct subfamilies based upon their sequence composition and phylogenetic positions. To further investigate the functions of these genes, we analyzed the chromosome distributions, gene structures, duplication patterns, phylogenetic tree, tissue expression patterns of the 189 AAT genes in soybean. We found that a large number of AAT genes in soybean were expanded via gene duplication, 46 and 36 GmAAT genes were WGD/segmental and tandemly duplicated, respectively. Further comprehensive analyses of the expression profiles of GmAAT genes in various stages of vegetative and reproductive development showed that soybean AAT genes exhibited preferential or distinct expression patterns among different tissues. Overall, our study provides a framework for further analysis of the biological functions of AAT genes in either soybean or other crops.

[Tissue distribution on methyl nonyl ketone of the volatile oil from Houttyunia cordata in mice].

OBJECTIVE: To study the tissue distribution on methyl nonyl ketone of the volatile oil from Houttuynia cordata in mice in order to provide a guidance for the clinical trial. METHODS: The concentrations of the volatile oil from Houttuynia cordata in biological samples were determined by GC method. RESULTS: After a single oral dose of 5.0 g/kg volatile oil from Houttuynia cordata in mice, parent drug was mainly distributed in windpipe, intesting, liver, kidney, heart, blood, spleen, lung, brain, muscle; after 7 hours, parent drug of every tissue decreased over 90%. CONCLUSION: Parent drug mianly distributes in windpipe, intestine, liver, kidney. The experiment provides pharmacokinetic evidence for the rational administration and the further development of Houttuynia cordata.