Performance of a continuous flow reactor on bio-reducing vanadium with straw.

Vanadium (V) in groundwater could cause a serious threat to the environment and health. Continuous flow reactors were applied to reduce V(V) with straw being a solid carbon. The reduced efficiency of V(V) in the reactor with straw and inoculated sludge reached to 71.8%-99.9% for two months' operation (after 44 d). However, a long-term operation with only straw was not satisfied, achieving the reduced efficiency of 39.2-66.6%. The SEM images clearly revealed some traces of straw surface by utilized by microbes, which implied that microbes had a stronger capacity to hydrolyze straw. The introducing external microbes were essential to achieve a better bio-reduction performance on V(V). Treponema (5.3%) with metal reduction ability and Prevotellaceae (3.3%) able to specifically degrade complex plant-derived polysaccharides were found to be dominant in the microbial community. Utilizing agricultural biomass can save a lot of normal carbon like acetate, which is of benefit for carbon emissions.

Extracting compositional blocks of alginate-like extracellular polymers (ALE) from conventional activated sludge (CAS).

As a highly added value material, alginate-like extracellular polymers (ALE) can be extracted from extracellular polymeric substances (EPS) from aerobic granular sludge (AGS). In fact, conventional activated sludge (CAS) also contains a certain amount of ALE. As CAS is widely used everywhere, waste activated sludge (WAS) from CAS is huge in its absolute amount. Although the ALE property of CAS was identified not so good as that from AGS, the mechanisms remains unclear. For this reason, it is necessary to unravel the chemically compositional blocks of ALE. Referring to natural alginate, ALE can be separated into three compositional blocks: GGL, GML and MML (like units containing guluronate or mannuronate), associated with other compositions including protein (PN), polysaccharide (PS), phosphorus (P), humic acid (HA). With real WAS from CAS, ALE was extracted and three blocks were separated: GGL = 54 %, GML = 42 % and MML = 4 % in weight, which is similar to the previous study. Moreover, the GGL blocks in CAS were obviously lower than AGS, down to by 1/3-1/2. And the GML and MML blocks in CAS were much higher than AGS, by more than 1/2. Different compositional blocks of ALE in AGS and CAS should be the reason forming different properties in applications. For this reason, a further study will be initiated to dispense/reorganize three blocks of ALE from CAS for expanding its potential applications, based on the compositional blocks of ALE from AGS.

Performance and Enhancement of Various Fillers Guiding Vanadium (V) Bioremediation.

Bioremediation of vanadium (V) pollution in groundwater is an emerging topic. However, knowledge of V in a biogeochemical process is limited and long-term effective removal methods are lacking. V(V) remediation processes by various kinds of auxiliary fillers (maifanite-1, maifanite-2, volcanic rock, green zeolite and ceramsite), agricultural biomass and microbial enhancing were explored in this study. In tests without inocula, the V(V) removal efficiencies of ceramsite (inert filler) and maifanite-2 (active filler) were 84.9% and 60.5%, respectively. When inoculated with anaerobic sludge, 99.9% of V(V) could be removed with the synergistic performance of straw and maifanite-2. TOC (Total Organic Carbon), trace elements and three-dimensional fluorescence analyses confirmed that maifanite-2 was the most suitable among various fillers in biological V(V) removal systems with straw. This study provides a collaborative method (adsorption-biology) by using straw with maifanite-2 in V(V)-contaminated groundwater. The knowledge gained in this study will help develop permeable reactive barrier technology to repair polluted groundwater to put forward a reasonable, effective and sustainable environmental treatment strategy.

Optimizing the added ratio of mixed auxiliary packings for enhancing the biological vanadium (V) removal.

Developing sustainable and low-cost bio-reduction technologies is essential for vanadium (V) bioremediation in groundwater. With both agricultural waste (wheat stalk) being a solid carbon source and ceramsite and medical stone being auxiliary packings, V(V) removal was confirmed and optimized in this study. The ratio of ceramsite to medical stone was maintained at 1:3 in Group I, which accomplished a V(V) removal efficiency up to 97.5% within 120 h and an average removal rate was around 0.305 mg/(L·h). The dissolution and utilization of carbon and trace elements (Mg, Fe, Mo and Ni) by microbes also contributed to the V(V) bio-reduction enhancement. The main components of DOM (tryptophan and humic acid-like substances) were vital in the V(V) binding and electron transfer processes. This study could promote the current knowledge on the sustainable V(V) bioremediation by using agricultural waste and auxiliary packings.

Biologically removing vanadium(V) from groundwater by agricultural biomass.

Vanadium (V) in groundwater can pose a serious threat on both environment and health. Agricultural biomass contains solid carbon source (SCS) and could be attractive for biologically removing V(V). For this purpose, cypress sawdust, corn cob and wheat straw were selected as SCSs to remove vanadate (NaVO3). The experiments demonstrated a high efficiency of V(V) up to 98.6%, and the anaerobically biological reduction of V(V) to V(IV) by wheat straw was identified to be the best SCS by the spectrum analysis of XRD and FTIR. Along with increasing the fragment size of wheat straw, the V(V)-removal efficiency decreased, and the fragment size down to 1-3 mm was confirmed to have a significant bio-removal performance on V(V). Based on the analysis of 16s rRNA sequencing, the microbial abundance and diversity increased in the suspension liquid in the end, indicating that the microbial community could tolerate and/or detoxify V(V), besides degrading lignocellulosic materials.

Performance and mechanisms for V(v) bio-reduction by straw: key influencing factors.

A high concentration of vanadium [V(v)] in groundwater is extremely harmful for humans. Weak movability and low toxicity after microbial V(v) reduction have attracted remarkable attention, especially for using solid carbon sources. However, the influencing factors remain unclear. In this study, the initial V(v) concentration, inocula amount and straw dosage were examined to ascertain the mechanisms behind them. Increasing the initial V(v) concentration led to the decrease of the V(v) removal efficiency, which was also positively correlated with the straw dosage within a certain range. The initial sludge amount was not a main factor affecting microbial V(v) removal in this study. With the initial amount of 10 mg L-1 V(v), 25 mL initial inocula and 5 g straw, 88.2% of V(v) was removed. According to the dissolved organic matter (DOM) analysis results, microbial activity prevailed in groups with higher V(v) removal efficiency, indicating that the V(v) bio-reduction was attributed to the microbial activity, which was considered a major factor. Functional species as unclassified_f_Enterobacteriaceae presumably contributed to the V(v) bioreduction, with upregulated ABC transporter genes and enzymes.

Human health risk of vanadium in farmland soils near various vanadium ore mining areas and bioremediation assessment.

Various kinds of vanadium (V) ore mining areas produced serious contamination have been widely recognized, while less relevant research was about the associated health risk and V distribution level for farmland soils around. This study assessed the contamination characteristics and associated human health risk of V in the surface farmland soils near various V ore mining areas. The bioremediation of V contamination by indigenous microbes from them was also evaluated. The farmland soils near stone coal area (Hunan province, China) showed the highest mean concentration of V (543.91 mg/kg), posing high non-carcinogenic risks, with high hazard quotient (HQ) value of 1.29 for children. While, V values of sampled soils near V titanomagnetite, petroleum associated minerals and uvanite areas were lower than that near stone coal area, also with lower HQ values (<1.00). Within 60 h, the removal efficiency of V(V) reached 98.4% with farmland soils near uvanite area, suggesting feasibility of V bioremediation via indigenous microbes. Bacterial communities after long-term cultivation (240 d) with V(V) were dominated by native microbes able to tolerate or reduce the toxicity of V(V), such as Ruminococcaceae_incertae_sedis, Trichococcus and Comamonas. This work is helpful for calling attention to V pollution of farmland near various V ore mining areas and formulating effective strategies for V(V) contamination bioremediation.

Microbial removal of vanadium (V) from groundwater by sawdust used as a sole carbon source.

Bioremediation of vanadium (V) (V(V)) for polluted groundwater is an emerging topic globally. With this study, microbial removal of V(V) was investigated by sawdust of pine used as a sole carbon source. The removal efficiency of V(V) reached up to 90.3% with anaerobic sludge as inocula and sawdust as the carbon source in nutrient solution. Microbial removal of V(V) could be enhanced by adding medical stone and phosphate rock, from 53.2% up to 82.6% in real groundwater. Microbiological analysis revealed such microbes as Thauera accumulated, which could contribute to V(V) reduction. Such functional species as Bacteroidetes vadinHA17 norank and Anaerolineaceae norank helped degradation of sawdust. In column experiments with domesticated sludge or indigenous microbes from soils, microbial V(V) removal efficiencies (on 26 d) with sawdust were around 58.7% (BS), 54.8% (BP) and 38.4% (BU), respectively. The study can offer a potential approach to microbially removing V(V) for contaminated groundwater and even for disposal of agricultural and forestry wastes.

Optical OCDMA coding and 3D imaging technique for non-scanning full-waveform LiDAR system.

Full-waveform LiDAR systems have been developed owing to their superiority in terms of high precision and resolution. However, further improving the imaging speed and resolution continues to be a problem. In this study, we propose an optical orthogonal code division multiple access (OCDMA) coding and 3D imaging technique, and a non-scanning full-waveform LiDAR system is first demonstrated. Encoding, multiplexing, and decoding are the essential modules of the OCDMA LiDAR system, which use M detectors and N-bits code to achieve high-accuracy decomposition for ${\rm M} \times {\rm N}$M×N pixels. In this paper, a complete 3D imaging LiDAR system is introduced, and the implementation of encoding and decoding is also illustrated. To prove that the technology is scientific and effective, an imaging experiment is carried out. The experimental result indicates that a system with only four avalanche photodiodes (APDs) can achieve 256 pixels. Moreover, the vertical resolution is about 1.8 cm and the range resolution is 15 cm at the distance of 40 m.

Adjustable higher SNR and long-range 3D-imaging cluster lidar based on a coded full-waveform technique.

Three-dimensional (3D) lidar imaging technology is developing rapidly in civil, commercial, and military fields. Especially in the military field, lidar with a high signal-to-noise ratio (SNR) and long range is essential. However, high SNR is contradictory to long-range detection, which is a difficult problem to be resolved. In this paper, we propose an adjustable higher SNR and long-range 3D-imaging cluster lidar (C-Lidar) based on a coded full-waveform technique. C-Lidar makes full use of linear mode avalanche photodiode (Lm-APD) multiplexing and space-time domain laser codec technology. The system uses only an 8×8 Lm-APD array to acquire 64×64×64 pixels in a single imaging process within 6.4×10-3 s. Based on the C-Lidar system, we establish a mathematical model to analyze the increased SNR by 18 dB. It is noteworthy that the SNR can be increased much higher when the product of the number of Lm-APDs and the length of the encoding sequence increase. The SNR is adjusted with the product, and C-Lidar changes the SNR by controlling the product. To verify the feasibility of the above theory, we conduct a principle prototype imaging experiment, and the result shows that C-Lidar achieves a clear image at 2 km.

Effects of levofloxacin exposure on sequencing batch reactor (SBR) behavior and microbial community changes.

The adaptation mechanisms of bacterial community for nitrogen removal performance exposed to fluctuated levels of levofloxacin (LVX) during wastewater treatment in SBRs were investigated. Although LVX is completely synthetic, the results of minimum inhibitory concentration (MIC, 32 mg-LVX/L) and minimum bactericidal concentration (MBC, 512 mg-LVX/L) of the sampled sludge showed that the LVX resistance/tolerance for bacterial growth has already existed in the actual wastewater treatment plants (WWTPs). The key bacteria, i.e. Nitrosomonas sp. (ammonia-oxidizing bacteria), Nitrospira sp. (nitrite-oxidizing bacteria) and Thauera sp. (the predominant denitrifiers), decreased with LVX exposure, and the recovery of biological process in the reactor was disturbed due to LVX exposure. However, after stopping exposure their population was quickly increased and thus the performance was recovered. The results of the non-metric multidimensional scaling and microbial community by sequencing showed the LVX concentration was a crucial factor to the change of bacterial communities and controlled the quantitative evolution of the communities in our systems. This effect was more pronounced as the LVX concentration was higher. The results suggested the removal of residual antibiotics to accomplish under no effect concentration before biological treatment is important to suppress emerging and increasing of the antibiotic resistant bacteria in WWTPs.

Microbial vanadium (V) reduction in groundwater with different soils from vanadium ore mining areas.

This work investigated the potential of vanadium (V) (V(V)) bioreduction by using soils sampled from four main kinds of vanadium ore mining areas, i.e. vanadium titanomagnetite, stone coal, petroleum associated minerals and uvanite as inocula. During a typical operation cycle of 60 h, the soils from vanadium titanomagnetite area and petroleum associated minerals area exhibited higher V(V) removal efficiencies, about 92.0 ±â€¯2.0% and 91.0 ±â€¯1.9% in comparison to 87.1 ±â€¯1.9% and 69.0 ±â€¯1.1% for the soils from uvanite and stone coal areas, respectively. Results from high-throughput 16 S rRNA gene pyrosequencing analysis reflect the accumulation of Bryobacter and Acidobacteriaceae with capabilities of V(V) reduction, accompanied with other functional species. This study is helpful to search new functional species for V(V) reduction and to develop in situ bioremediations of V(V) polluted groundwater.

Effects of various organic carbon sources on simultaneous V(V) reduction and bioelectricity generation in single chamber microbial fuel cells.

Four ordinary carbon sources affecting V(V) reduction and bioelectricity generation in single chamber microbial fuel cells (MFCs) were investigated. Acetate supported highest maximum power density of 589.1mW/m(2), with highest V(V) removal efficiency of 77.6% during 12h operation, compared with glucose, citrate and soluble starch. Exorbitant initial V(V) concentration led to lower V(V) removal efficiencies and power outputs. Extra addition of organics had little effect on the improvement of MFCs performance. V(V) reduction and bioelectricity generation were enhanced and then suppressed by the increase of conductivity. The larger the external resistance, the higher the V(V) removal efficiencies and voltage outputs. High-throughput 16S rRNA gene pyrosequencing analysis implied the accumulation of Enterobacter which had the capabilities of V(V) reduction, electrochemical activity and fermentation, accompanied with other functional species as Pseudomonas, Spirochaeta, Sedimentibacter and Dysgonomonas. This study steps forward to remediate V(V) contaminated environment based on MFC technology.

Spontaneous arsenic (III) oxidation with bioelectricity generation in single-chamber microbial fuel cells.

Arsenic is one of the most toxic elements commonly found in groundwater. With initial concentration of 200µgL(-1), spontaneous As(III) oxidation is realized completely during 7 days operation in single-chamber microbial fuel cells (MFCs) in the present study, with the maximum power density of 752.6±17mWm(-2). The product is less toxic and mobile As(V), which can be removed from aqueous solution more easily. High-throughput 16S rRNA gene pyrosequencing analysis indicates the existence of arsenic-resistant bacteria as Actinobacteria, Comamonas, Pseudomonas and arsenic-oxidizing bacteria as Enterobacter, with electrochemically active bacteria as Lactococcus, Enterobacter. They interact together and are responsible for As(III) oxidation and bioelectricity generation in MFCs. This study offers a potential attractive method for remediation of arsenic-polluted groundwater.

Microbial reduction and precipitation of vanadium (V) in groundwater by immobilized mixed anaerobic culture.

Vanadium is an important contaminant impacted by natural and industrial activities. Vanadium (V) reduction efficiency as high as 87.0% was achieved by employing immobilized mixed anaerobic sludge as inoculated seed within 12h operation, while V(IV) was the main reduction product which precipitated instantly. Increasing initial V(V) concentration resulted in the decrease of V(V) removal efficiency, while this index increased first and then decreased with the increase of initial COD concentration, pH and conductivity. High-throughput 16S rRNA gene pyrosequencing analysis indicated the decreased microbial diversity. V(V) reduction was realized through dissimilatory reduction process by significantly enhanced Lactococcus and Enterobacter with oxidation of lactic and acetic acids from fermentative microorganisms such as the enriched Paludibacter and the newly appeared Acetobacterium, Oscillibacter. This study is helpful to detect new functional species for V(V) reduction and constitutes a step ahead in developing in situ bioremediations of vanadium contamination.

Simultaneous microbial and electrochemical reductions of vanadium (V) with bioelectricity generation in microbial fuel cells.

Simultaneous microbial and electrochemical reductions of vanadium (V) with bioelectricity generation were realized in microbial fuel cells (MFCs). With initial V(V) concentrations of 75 mg/l and 150 mg/l in anolyte and catholyte, respectively, stable power output of 419±11 mW/m(2) was achieved. After 12h operation, V(V) concentration in the catholyte decreased to the value similar to that of the initial one in the anolyte, meanwhile it was nearly reduced completely in the anolyte. V(IV) was the main reduction product, which subsequently precipitated, acquiring total vanadium removal efficiencies of 76.8±2.9%. Microbial community analysis revealed the emergence of the new species of Deltaproteobacteria and Bacteroidetes as well as the enhanced Spirochaetes mainly functioned in the anode. This study opens new pathways to successful remediation of vanadium contamination.