Mobile nursing care plan information system for nursing service in hospitals.

OBJECTIVE: The COVID-19 pandemic has greatly affected the health sector, especially the speed of healthcare services in hospitals, such as nursing care. This is caused by the low ratio between the number of resources available and the number of cases handled, which leads to suboptimal services. One of the solutions to this problem is to implement a digital (mobile) nursing care information system. Therefore, this study aims to produce a digital (mobile) nursing information system model that is suitable for hospitals. SUBJECTS AND METHODS: The research and development (RnD) method used in this study was carried out in three stages, namely, analysis of information system needs, preparation of the model design, and trial of Mobile Nursing Care Plan Information System (MNCPIS). The design was prepared with the Framework for Application of System Technique (FAST). A total of 148 nurses were selected as respondents using a simple random sampling approach. Data were then collected through interviews, focus group discussions, and questionnaires. Furthermore, MNCPIS was assessed with system requirements analysis using the Performance, Information, Economic, Control/Security, Efficiency, and Service (PIECES) framework. RESULTS: The t-test results indicate that MNCPIS was more effective and appropriate compared to the conventional (manual) types. This was indicated by all the variables measured in the MNCPIS component, including assessment, nursing diagnosis and evaluation, planning, and implementation. Based on these findings, the model can be developed as a nursing care information system to improve the quality assurance of services rendered by nurses. CONCLUSIONS: A mobile plan information system can be implemented in the nursing care department in hospitals to ensure satisfaction and quality assurance for the services provided in hospitals.

Controlled expression of cholera toxin B subunit from Vibrio cholerae in Escherichia coli.

The ctxB gene, the causative agent of cholera epidemic was successfully cloned from V. cholerae in E. coli. The insertion of the gene was confirmed by PCR as well as restriction digestion analyses. The sequencing results for the gene confirmed that the insert was in the correct orientation and in-frame with the P(BAD) promoter and it showed that the gene was 99% homologous to the published ctxB sequence. The CTB protein was successfully expressed in E. coli using the pBAD/His vector system. The expected protein of approximately 14 kDa was detected by SDS-PAGE and Western blot. The use of pBAD/His vector to express the cholera toxin gene in E. coli would facilitate future study of toxin gene products.

Expression of a thermostable xylanase gene from Bacillus coagulans ST-6 in Lactococcus lactis.

AIMS: The aim of the study is to evaluate whether xylanase can be used as a potential reporter gene for cloning and expression studies in Lactococcus. METHODS AND RESULTS: The 750 bp xylanase gene was amplified and subcloned into the unique NheI restriction enzyme site of pMG36e and subsequently transformed into competent Escherichia coli XLI-blue MRF cells and Lactococcus lactis cells. Bacterial culture containing pMG36e-Xy has an enzyme activity of 390 microg xylose ml(-1) culture 30 min(-1), respectively, when compared with 40 microg xylose ml(-1) culture 30 min(-1) for the negative control (plasmidless strain). CONCLUSIONS: The thermostable xylanase gene was successfully expressed in both E. coli and L. lactis. The activity of xylanase can be easily detected by the formation of visible clearing zones around the transformed colonies on Remazol Brilliant Blue-Xylan (RBB-Xylan) agar media. However, there were some significant differences in the optimum growth temperature and plasmid stability in the new clones. SIGNIFICANCE AND IMPACT OF THE STUDY: The constructed reporter vector has the potential to be used as a reporter system for Lactococcus as well as E. coli, and it is an addition to the pool of lactococcal vector systems.