Influence of bioaugmented fungi on tolerance, growth and phytoremediation ability of Prosopis juliflora Sw. DC in heavy metal-polluted landfill soil.

The research aimed to determine the influence of endophytic fungi on tolerance, growth and phytoremediation ability of Prosopis juliflora in heavy metal-polluted landfill soil. A consortium of 13 fungal isolates as well as Prosopis juliflora Sw. DC was used to decontaminate heavy metal-polluted landfill soil. Enhanced plant growth (biomass and root and shoot lengths) and production of carotenoids, chlorophyll and amino acids L-phenylalanine and L-leucine that are known to enhance growth were found in the treated P. juliflora. Better accumulations of heavy metals were observed in fungi-treated P. juliflora over the untreated one. An upregulated activity of peroxidase, catalase and ascorbate peroxidase was recorded in fungi-treated P. juliflora. Additionally, other metabolites, such as glutathione, 3,5,7,2',5'-pentahydroxyflavone, 5,2'-dihydroxyflavone and 5,7,2',3'-tetrahydroxyflavone, and small peptides, which include Lys Gln Ile, Ser Arg Ala, Asp Arg Gly, Arg Ser Ser, His His Arg, Arg Thr Glu, Thr Arg Asp and Ser Pro Arg, were also detected. These provide defence supports to P. juliflora against toxic metals. Inoculating the plant with the fungi improved its growth, metal accumulation as well as tolerance against heavy metal toxicity. Such a combination can be used as an effective strategy for the bioremediation of metal-polluted soil.

Valorisation of keratinous wastes: A sustainable approach towards a circular economy.

The valorisation of keratinous wastes involves biorefining and recovering the bioresource materials from the keratinous wastes to produce value-added keratin-based bioproducts with a broad application, distribution, and marketability potential. Valorisation of keratinous wastes increases the value of the wastes and enables more sustainable waste management towards a circular bioeconomy. The abundance of keratinous wastes as feedstock from agro-industrial processing, wool processing, and grooming industry benefits biorefinery and extraction of keratins, which could be the optimal solution for developing an ecologically and economically sustainable keratin-based economy. The transition from the current traditional linear models that are deleterious to the environment, which end energy and resources recovery through disposal by incineration and landfilling, to a more sustainable and closed-loop recycling and recovery approach that minimises pollution, disposal challenges, loss of valuable bioresources and potential revenues are required. The paper provides an overview of keratinous wastes and the compositional keratin proteins with the descriptions of the various keratin extraction methods in biorefinery and functional material synthesis, including enzymatic and microbial hydrolysis, chemical hydrolysis (acid/alkaline hydrolysis, dissolution in ionic liquids, oxidative and sulphitolysis) and chemical-free hydrolysis (steam explosion and ultrasonic). The study describes various uses and applications of keratinases and keratin-based composites fabricated through various manufacturing processes such as lyophilisation, compression moulding, solvent casting, hydrogel fabrication, sponge formation, electrospinning, and 3D printing for value-added applications.