A field-portable electrochemical immunosensor based on a multifunctional Ag2O/g-C3N4@MA-DBB covalent organic framework receptor interface for single-step detection of aflatoxin M1 in raw milk samples.

Aflatoxin M1 (AFM1), a hydroxylated metabolite of aflatoxin B1 (AFB1), contaminates milk and dairy products from livestock via ingestion of feed contaminated with a species of Aspergillus. As AFM1 is a Group-II B carcinogen, it is indispensable to develop novel, easy-to-handle, sensitive, portable and cost-effective strategies for its detection. Herein, a covalent organic framework (COF)-based electroactive nanocomposite, Ag2O/g-C3N4-COOH@MA-DBB-COF (silver oxide/carboxy-functionalized graphitic carbon nitride@melamine-dibromo butane COF), is designed to serve as a multifunctional receptor surface. The Ag2O/g-C3N4-COOH@MA-DBB-COF formation was characterized through X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, micro-Raman spectroscopy (RAMAN), dynamic light scattering (DLS) and thermogravimetric analysis (TGA), and each step of the sensor fabrication was monitored using field emission scanning electron microscopy (FE-SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimal conditions, the designed immunosensor permitted the detection of AFM1 in the linear range of 0.03-1000 fg mL-1, with a 0.01 fg mL-1 limit of detection (LOD). The selectivity of the designed immunosensor was validated via an anti-interference study. The practical applicability of the immunosensor was demonstrated by the detection of AFM1 in real milk samples, and good recovery values (97.28-102.62%) were obtained. Furthermore, the developed immunosensor and high-performance liquid chromatography (HPLC) were employed in parallel to detect AFM1 in local market milk samples from twenty different sites to validate the performance of the newly designed immunosensor. Additionally, the designed immunosensor was stable over an extended period of time. This work reports a single-step field-portable multifunctional innovative electrochemical immunoreceptor design for on-site and label-free detection of AFM1 in milk samples. Hence, the present study is the first report on the fabrication of a multifunctional innovative electrochemical immunoreceptor based on PGE/Ag2O/g-C3N4-COOH@MA-DBB-COF for the detection of AFM1 in milk samples.

A multifunctional N-GO/PtCo nanocomposite bridged carbon fiber interface for the electrochemical aptasensing of CA15-3 oncomarker.

The development of integrated analytical devices is crucial for advancing next-generation point-of-care platforms. Herein, we describe a facile synthesis of a strongly catalytic and durable Nitrogen-doped graphene oxide decorated platinum cobalt (NGO-PtCo) nanocomposite that is conjugated with target-specific DNA aptamer (i-e. MUC1) and grown on carbon fiber. Benefitting from the combined features of the high electrochemical surface area of N-doped GO, high capacitance and stabilization by Co, and high kinetic performance by Pt, a robust, multifunctional, and flexible nanotransducer surface was created. The designed platform was applied for the specific detection of a blood-based oncomarker, CA15-3. The electrochemical characterization proved that nanosurface provides a highly conductive and proficient immobilization support with a strong bio-affinity towards MUC1 aptamer. The specific interaction between CA15-3 and the aptamer alters the surface properties of the aptasensor and the electroactive signal probe generated a remarkable increase in signal intensity. The sensor exhibited a wide dynamic range of 5.0 × 10-2 -200 U mL-1, a low limit of detection (LOD) of 4.1 × 10-2 U mL-1, and good reproducibility. The analysis of spiked serum samples revealed outstanding recoveries of up to 100.03 %, by the proposed aptasensor. The aptasensor design opens new revelations in the reliable detection of tumor biomarkers for timely cancer diagnosis.

Fabrication of a salivary amylase electrochemical sensor based on surface confined MWCNTs/ß-cyclodextrin/starch architect for dental caries in clinical samples.

Salivary α-amylase (α-ALS) has drawn attention as a possible bioindicator for dental caries. Herein, combining the synergistic properties of multi-walled carbon nanotubes (MWCNTs), ß-cyclodextrin (ß-CD) and starch, an electrochemical sensor is constructed employing ferrocene (FCN) as an electrochemical indicator to oversee the progression of the enzymatic catalysis of α-ALS. The method involves a two-step chemical reaction sequence on a screen-printed carbon electrode (SPCE). X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, Field emission scanning electron microscope (FE-SEM), and Dynamic light scattering (DLS) were used to characterize the synthesized material, while Static water Contact angle measurements, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) were performed to monitor each step of sensor fabrication. The electrochemical sensor permitted to detect α-ALS within the linear range of 0.5-280 U mL-1, revealing detection (LOD), and quantification (LOQ) values of 0.041 U mL-1, and 0.159 U mL-1, respectively. Remarkably, the sensor demonstrated exceptional specificity and selectivity, effectively discriminating against other interfering substances in saliva. Validation of the method involved analyzing α-ALS levels in artificial saliva with an accuracy range of 97 % to 103 %, as well as in real clinical saliva samples across various age groups.

Gastroenteropancreatic neuroendocrine neoplasms: current development, challenges, and clinical perspectives.

Neuroendocrine neoplasms (NENs) are highly heterogeneous and potentially malignant tumors arising from secretory cells of the neuroendocrine system. Gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) are the most common subtype of NENs. Historically, GEP-NENs have been regarded as infrequent and slow-growing malignancies; however, recent data have demonstrated that the worldwide prevalence and incidence of GEP-NENs have increased exponentially over the last three decades. In addition, an increasing number of studies have proven that GEP-NENs result in a limited life expectancy. These findings suggested that the natural biology of GEP-NENs is more aggressive than commonly assumed. Therefore, there is an urgent need for advanced researches focusing on the diagnosis and management of patients with GEP-NENs. In this review, we have summarized the limitations and recent advancements in our comprehension of the epidemiology, clinical presentations, pathology, molecular biology, diagnosis, and treatment of GEP-NETs to identify factors contributing to delays in diagnosis and timely treatment of these patients.

Covalent organic frameworks (COFs) as carrier for improved drug delivery and biosensing applications.

Porous organic frameworks (POFs) represent a significant subclass of nanoporous materials in the field of materials science, offering exceptional characteristics for advanced applications. Covalent organic frameworks (COFs), as a novel and intriguing type of porous material, have garnered considerable attention due to their unique design capabilities, diverse nature, and wide-ranging applications. The unique structural features of COFs, such as high surface area, tuneable pore size, and chemical stability, render them highly attractive for various applications, including targeted and controlled drug release, as well as improving the sensitivity and selectivity of electrochemical biosensors. Therefore, it is crucial to comprehend the methods employed in creating COFs with specific properties that can be effectively utilized in biomedical applications. To address this indispensable fact, this review paper commences with a concise summary of the different methods and classifications utilized in synthesizing COFs. Second, it highlights the recent advancements in COFs for drug delivery, including drug carriers as well as the classification of drug delivery systems and biosensing, encompassing drugs, biomacromolecules, small biomolecules and the detection of biomarkers. While exploring the potential of COFs in the biomedical field, it is important to acknowledge the limitations that researchers may encounter, which could impact the practicality of their applications. Third, this paper concludes with a thought-provoking discussion that thoroughly addresses the challenges and opportunities associated with leveraging COFs for biomedical applications. This review paper aims to contribute to the scientific community's understanding of the immense potential of COFs in improving drug delivery systems and enhancing the performance of biosensors in biomedical applications.

An ALP enzyme-based electrochemical biosensor coated with signal-amplifying BaTiO3 nanoparticles for the detection of an antiviral drug in human blood serum.

Tenofovir (TFV) is an antiviral drug used to treat the co-infections of HIV/HBV viruses. Accurate monitoring of TFV drug levels is essential for evaluating patient adherence, optimizing dosage, and assessing treatment efficacy. Herein, we propose an innovative electrochemical sensing approach by using the alkaline phosphatase (ALP) enzyme with the support of BaTiO3 nanoparticles. An attractive sensitivity and selectivity of the developed sensor towards TFV detection were achieved. First, the nanoparticles were synthesized by following a single-step sol-gel method and characterized through various analytical techniques, including SEM, EDX, FT-IR, BET, zeta potential, XRD, and UV-vis and Raman spectroscopy. The suggested mechanism demonstrated the formation of a strong bond between TFV and the ALP enzyme, primarily through the phosphate group, resulting in enzyme inhibition. Various parameters like nanoparticle amount, electrode modification time with enzyme and BaTiO3 nanoparticles, and drug incubation time were optimized. The biosensor demonstrated an outstanding limit of detection (LOD) of 0.09 nM and recovery percentages of 98.6-106% in human blood serum, indicating adequate repeatability and selectivity. The proposed biosensor can be converted into a portable device for measuring small sample volumes and observing patients for immediate medical care or personalized therapies. It achieved better sensitivity compared to existing methods, making it suitable for precise drug detection in microdoses.

Host-Guest Mechanism via Induced Fit Fullerene Complexation in Porphin Receptor to Probe Salivary Alpha-Amylase in Dental Caries for Clinical Applications.

Salivary α-amylase is the most abundant protein of human saliva that potentially binds to streptococcus and other bacteria via specific surface-exposed α-amylase-binding proteins and plays a significant role in caries development. The detection of α-amylase in saliva can be used as a bioindicator of caries development. Herein, a facile strategy has been applied, tailoring the photochemical properties of 5,10,15,20-tetrakis(4-hydroxyphenyl)-21H,23H-porphine (TPPOH) and the fullerene C60 complex. The fluorescence emission of TPPOH is quenched by starch-coated fullerene C60 via charge-transfer effects, as determined by UV absorption and fluorescence spectroscopic studies. The starch-coated C60 has been thoroughly characterized via Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), optical microscopy, thermal gravimetric analysis (TGA), static water contact angle measurements, and zeta potential measurements. The analytical response of the assay showed a linear fluorescent response in α-amylase concentrations ranging from 0.001-0.1 Units/mL, with an LOD of 0.001 Units/mL. The applicability of the method was tested using artificial saliva with quantitative recoveries in the range 95-100%. The practicability of the procedure was verified by inspecting saliva samples of real clinical samples covering all age groups. We believe that the proposed method can serve as an alternative analytical method for caries detection and risk assessment that would also minimize the cost of professional preventive measures and treatments.

Growth of diazonium-functionalized ZnO nanoflakes on flexible carbon cloth for electrochemical sensing of acetone in the liquid phase.

Simple detection of acetone is indispensable due to its health and environmental concerns. Surface-modified electrodes are promising for the detection of acetone. In the present study, the facile fabrication of ZnO nanoflakes on carbon cloth (CC) is reported. The electrode was fabricated by decorating the CC with ZnO nanoparticles (ZnO NPs), followed by the hydrothermal treatment and modification with diazonium salt using linear sweep voltammetry (LSV) forming ZnO nanoflakes (ZnO NFs) on ZnO NPs/CC. The as-prepared ZnO/CC electrode was used for the detection of acetone at room temperature using cyclic voltammetry. Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Brunauer-Emmett-Teller (BET) analyses were used for the chemical and physical characterization of the CC before and after each modification step. The obtained data manifested that ZnO NFs functionalized with diazonium salt increased the roughness of the CC surface, which was advantageous to promote the interaction between CC and acetone target. The modified sensing platform showed excellent performance in terms of the wide working range (0.1-2000 ppm) and low detection limit (0.03 ppm), making it a promising and cost-effective sensor of acetone in the liquid phase.

Fabrication of polydopamine decorated carbon cloth as support material to anchor CeO2 nanoparticles for electrochemical detection of ethanol.

A flexible CeO2 nanostructured polydopamine-modified carbon cloth (CeO2/PDA/CC) interface was fabricated via electrodeposition for ethanol detection. The fabrication method involved two consecutive electrochemical steps in which dopamine was firstly electrodeposited on carbon fibers, followed by the electrochemical growth of CeO2 nanoparticles. The CeO2/PDA-based electroactive interface exerts an impressive electrochemical performance on the flexible sensor due to strong synergistic effect of the PDA functionalization with more active sites. Moreover, catalytic activity of CeO2 nanostructures anchored on highly conductive CC incorporate superior electrocatalytic performance of the fabricated interface. The designed electrochemical sensor showed a wide response to ethanol in the linear range 1 to 25 mM with a detection limit of 0.22 mM. The CeO2/PDA/CC flexible sensor showed good anti-interference ability and excellent repeatability and reproducibility (RSD = 1.67%). The fabricated interface performed well in saliva samples with satisfactory recoveries, corroborating the viability of CeO2/PDA/CC integrated interface for practical implementation.

Cyclophosphazene Intrinsically Derived Heteroatom (S, N, P, O)-Doped Carbon Nanoplates for Ultrasensitive Monitoring of Dopamine from Chicken Samples.

A novel, metal-free electrode based on heteroatom (S, N, P, O)-doped carbon nanoplates (SNPO-CPL) modifying lead pencil graphite (LPG) has been synthesized by carbonizing a unique heteroatom (S, N, P, O)-containing novel polymer, poly(cyclcotriphosphazene-co-2,5-dioxy-1,4-dithiane) (PCD), for precise screening of dopamine (DA). The designed electrode, SNPO-CPL-800, with optimized percentage of S, N, P, O doping through the sp2-carbon chain, and a large number of surface defects (thus leading to a maximum exposition number of catalytic active sites) led to fast molecular diffusion through the micro-porous structure and facilitated strong binding interaction with the targeted molecules in the interactive signaling transducer at the electrode-electrolyte interface. The designed SNPO-CPL-800 electrode exhibited a sensitive and selective response towards DA monitoring, with a limit of detection (LOD) of 0.01 nM. We also monitored DA levels in commercially available chicken samples using the SNPO-CPL-800 electrode even in the presence of interfering species, thus proving the effectiveness of the designed electrode for the precise monitoring of DA in real samples. This research shows there is a strong potential for opening new windows for ultrasensitive DA monitoring with metal-free electrodes.

Graphene oxide reinforced silk fibroin nanocomposite as an electroactive interface for the estimation of dopamine.

The fabrication of 2D materials and polymer-based nanocomposites deposited on flexible conductive interfaces has unblocked new horizons to expedite reaction kinetics for developing highly selective and sensitive electrochemical biosensors. Herein, we developed a novel biosensing platform, comprising graphene oxide and a silk fibroin-based nanocomposite, drop-cast on a carbon cloth electrode. The fabricated interface was expected to be a robust and miniaturized sensing platform for precise detection of dopamine (DA). Characterization was performed by SEM, EDX, FTIR, XRD, UV-visible spectroscopy, contact angle measurement, fluorescence spectroscopy, particle size, and zeta potential analysis. CV, EIS, DPV, and chronoamperometry demonstrated the superior electrochemical properties of the working interface and revealed its enhanced active surface area, increased conductivity, and accelerated electron transfer rate. The designed interface exhibited low LoD (0.41 µM), admirable stability, good sensitivity (2.46 µA µM-1 cm-2), wide linearity ranging from 100-900 µM, excellent reproducibility, and superb selectivity against dopamine even in the presence of possible interfering analytes. These findings endorse the feasibility of the practical execution of such an integrated system in real sample analysis.

Multifunctional Smart ZnSe-Nanostructure-Based Fluorescent Aptasensor for the Detection of Ochratoxin A.

Herein, we present a comprehensive investigation of rationally designed zinc selenide (ZnSe) nanostructures to achieve highly negatively charged ZnSe nanostructures. A Microwave-assisted hydrothermal synthesis method was used to synthesize three types of ZnSe nanostructures, i.e., nanorods, µ-spheres and nanoclusters, as characterized by a zeta potential analyzer, X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman spectroscopy and BET, which were labeled as type A, B and C. Three different solvents were used for the synthesis of type A, B and C ZnSe nanostructures, keeping other synthesis conditions such as temperature, pressure and precursors ratio constant. Based on two heating time intervals, 6 and 9 h, types A, B and C were further divided into types A6, A9, B6, B9, C6 and C9. ZnSe nanostructures were further evaluated based on their fluorescent quenching efficiency. The maximum fluorescence quenching effect was exhibited by the ZnSe-B6 type, which can be attributed to its highly negative surface charge that favored its strong interaction with cationic dye Rhodamine B (Rh-B). Further, the optimized ZnSe-B6 was used to fabricate an aptasensor for the detection of a food-based toxin, ochratoxin-A (OTA). The developed aptasensor exhibited a limit of detection of 0.07 ng/L with a wide linear range of 0.1 to 200 ng/L.

N-Doped graphene quantum dots (N-GQDs) as fluorescent probes for detection of UV induced DNA damage.

UV induced DNA damage can lead to the development of skin cancer, skin aging and cell death. In this study, we fabricated a fluorescence-based biosensor that can be applied to the detection of DNA damage caused by UV radiation with the help of nitrogen doped graphene quantum dots (N-GQDs) as the probe material. In this paper, N-GQDs with good fluorescence efficiency have been synthesized by the hydrothermal method and were used as a fluorescent probe for the detection of UV damaged DNA. The fluorescence intensity of N-GQDs was quenched by the static quenching of UV damaged DNA through the formation of a N-GQD/UV damaged DNA complex. Moreover, the effects of different values of pH, NaCl and glucose on analytical performances of the sensor were also studied. Thus, using a fluorescence based approach, we demonstrated a quite simple, rapid, and inexpensive biosensor for the detection of DNA damage caused by UV radiation.

Black phosphorus nanosheets/poly(allylamine hydrochloride) based electrochemical immunosensor for the selective detection of human epididymis protein 4.

In this work, an electrochemical immunosensor based on black phosphorus nanosheets (BPNS)/poly(allylamine hydrochloride) (PAH) nanocomposite modified glassy carbon electrode was developed for the detection of ovarian cancer biomarker HE4. PAH has been applied to retain BPNS in its original honeycomb structure and to anchor biomolecules electrostatically on the transducer surface. The as synthesized nanocomposite was characterized by zeta potential analysis, scanning electron microscopy, x-ray photoelectron spectroscopy, transmission electron microscopy, high-resolution transmission electron microscopy. Subsequently, the performance of the electrochemical immunosensor was evaluated through cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. Under the optimal condition, the developed electrochemical immunosensor permitted to detect HE4 with a linear range of 0.1-300 ng ml-1and a detection limit of 0.01 ng ml-1. The developed sensor exhibited good selectivity and specificity to HE4 with negligible interference effect from common biomolecules like bovine serum albumin, lysozyme, protamine, glucose, fructose, hemoglobin and fetal bovine serum. Further, practical application of developed electrochemical immunosensor was demonstrated in spiked human serum which showed satisfactory recovery percentages.

Functionalized thiazolidone-decorated lanthanum-doped copper oxide: novel heterocyclic sea sponge morphology for the efficient detection of dopamine.

Herein, we synthesized lanthanum (La)-doped sea sponge-shaped copper oxide (CuO) nanoparticles and wrapped them with novel O-, N- and S-rich (2Z,5Z)-3-acetyl-2-((3,4-dimethylphenyl)imino)-5-(2-oxoindolin-3-ylidene)thiazolidin-4-one (La@CuO-DMT). The shape and composition of the designed materials were confirmed by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and Raman spectroscopy. The graphitic pencil electrode (GPE) fabricated using La@CuO-DMT showed excellent sensing efficacy against dopamine (DA) with good selectivity, reproducibility and ideal stability. The unique morphology and massive surface defects by La@CuO offer good accessibility to DA and enhance smooth and robust channeling of electrons at the electrode-electrolyte interface. Consequently, these properties resulted in improved reaction kinetics and robust DA oxidation with an amplified faradaic response. Meanwhile, O-, N-, and S-enriched carbon support, i.e. DMT, inhibited the leaching of electrode matrixes, resulting in a superior detection limit of 423 nm and an improved sensitivity of 13.9 µA µM-1 cm-2 in the linear range of 10 µM to 1500 µM. Additionally, the developed sensing interface was successfully employed to analyze DA from tear samples with excellent percentage recoveries. We expect that such engineered morphology-based nanoparticles with a O-, N-, and S-rich C support will facilitate the development of DA sensors for in vitro screening of rarely studied tear samples with good sensitivity and selectivity.

Non-enzymatic electrochemical sensing of dopamine from COVID-19 quarantine person.

The worldwide outbreak of COVID-19 pandemic, is not only a great threat to the victim life but it is leaving invisible devastating negative affect on mental health of quarantined individual because of isolation, depression, bereavement, and loss of income. Therefore, the precise monitoring catecholamine neurotransmitters specifically of dopamine (DA) is of great importance to assess the mental health. Thus, herein we have synthesized Co-based zeolitic imidazolate framework (ZIF-67) through solvothermal method for precise monitoring of DA. To facilitate the fast transportation of ions, highly conductive polymer, poly(3,4-ethylenedioxythiophene; PEDOT) has been integrated on the surface of ZIF-67 which not only provides the smooth pathway for ions/electrons transportation but also saves the electrode from pulverization. The fabricated ZIF-67/PEDOT electrode shows a significant sensing performance towards DA detection in terms of short diffusion pathways by expositing more active sites, over good linear range (15-240 µM) and a low detection limit of (0.04 µM) even in the coexistence of the potentially interfering molecules. The developed ZIF-67/PEDOT sensor was successfully employed for sensitive and selective monitoring of DA from COVID-19 quarantined person blood, thus suggesting reliability of the developed electrode.

A novel construct of an electrochemical acetylcholinesterase biosensor for the investigation of malathion sensitivity to three different insect species using a NiCr2O4/g-C3N4 composite integrated pencil graphite electrode.

Herein, an electrochemical biosensor has been prepared to assess the sensitivity of an organophosphate insecticide, malathion, to acetylcholinesterase (AChE) enzyme of three insects including Apis mellifera (honeybee), Tribolium castaneum (red flour beetle), and Zootermopsis nevadensis (dampwood termite). A composite of nickel chromite (NiCr2O4) and graphitic carbon nitride (g-C3N4) was prepared and characterized for its morphological, chemical and electrical properties. The NiCr2O4/g-C3N4 composite integrated pencil graphite electrodes were used to covalently immobilize insect AChE enzymes and amperometric response of bioelectrodes was determined through cyclic voltammetry. The prepared bioelectrodes exhibited high enzyme immobilization efficiency and electro-catalytic performance. The integrated bioelectrodes could efficiently detect malathion induced inhibition of insects' AChEs. The linear ranges for malathion were found to be 0.1-1.6 µM, 1-40 nM and 2-100 nM, and LODs were 2 nM, 0.86 nM and 2.3 nM for A. mellifera, T. castaneum, and Z. nevadensis, respectively. Additionally, the biosensing platform developed using A. mellifera AChE was found highly sensitive and effective for malathion recoveries from spiked wheat flour samples with high recovery rates. Moreover, the proposed method was adequately reproducible and selective. The results revealed that A. mellifera AChE is less sensitive to inhibition by malathion as compared to T. castaneum, and Z. nevadensis AChE. The experimental results were validated through computational docking of malathion with insect AChEs and the results were in correspondence to experimental outcomes. The proposed method can be a plausible alternate to conventional analytical methods to assess the pesticide sensitivity and toxicity of various compounds against insect enzymes.

Insight into prognostics, diagnostics, and management strategies for SARS CoV-2.

The foremost challenge in countering infectious diseases is the shortage of effective therapeutics. The emergence of coronavirus disease (COVID-19) outbreak has posed a great menace to the public health system globally, prompting unprecedented endeavors to contain the virus. Many countries have organized research programs for therapeutics and management development. However, the longstanding process has forced authorities to implement widespread infrastructures for detailed prognostic and diagnostics study of severe acute respiratory syndrome (SARS CoV-2). This review discussed nearly all the globally developed diagnostic methodologies reported for SARS CoV-2 detection. We have highlighted in detail the approaches for evaluating COVID-19 biomarkers along with the most employed nucleic acid- and protein-based detection methodologies and the causes of their severe downfall and rejection. As the variable variants of SARS CoV-2 came into the picture, we captured the breadth of newly integrated digital sensing prototypes comprised of plasmonic and field-effect transistor-based sensors along with commercially available food and drug administration (FDA) approved detection kits. However, more efforts are required to exploit the available resources to manufacture cheap and robust diagnostic methodologies. Likewise, the visualization and characterization tools along with the current challenges associated with waste-water surveillance, food security, contact tracing, and their role during this intense period of the pandemic have also been discussed. We expect that the integrated data will be supportive and aid in the evaluation of sensing technologies not only in current but also future pandemics.

Nickel -doped iron oxide nanoparticle-conjugated porphyrin interface (porphyrin/Fe2O3@Ni) for the non-enzymatic detection of dopamine from lacrimal fluid.

Herein, we synthesized nickel (Ni)-doped iron oxide nanoparticles (Fe2O3). The presence of the dopant afforded anchoring sites for the porphyrinic hetero cavity of 5,10,15,20-(tetra-4-carboxyphenyl)porphyrin to produce the porphyrin/Fe2O3@Ni composite. The crystalline structure and morphology of porphyrin/Fe2O3@Ni were assessed using various tools including Fourier transform spectroscopy (FTIR), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD) and Raman spectroscopy. Porphyrin/Fe2O3@Ni has proven to be an excellent dopamine (DA) probe material with good selectivity, reproducibility, stability and reliability owing to its clever morphology, which induces numerous active sites along with good active surface area. It consequently provides good accessibility to DA and allows for the smooth tunneling of electrons between the analyte and sensing interface. Meanwhile, the porphyrin molecules provide good carbon-based resilient support, inhibit the leaching of the electrode matrix and enhance electron shuttling, resulting in the robust oxidation of DA with amplified transduction signals. The designed porphyrin/Fe2O3@Ni interface showed a low detection limit (1.2 nm) with good sensitivity (1.2 nM) in the linear bounds of 10 µM to 3500 µM. Additionally, the interface was employed successfully to analyze DA from lacrimal fluid with good percentage recoveries (99.8% to 100.1%). We anticipate that such a design will simplify the in vitro screening of DA in rarely studied tear samples with sensitivity and selectivity.

Fabrication of ionic liquid stabilized MXene interface for electrochemical dopamine detection.

Development of MXene (Ti3C2Cl2)-based sensing platforms by exploiting their inherent active electrochemistry is highly challenging due to their characteristic poor stability in air and water. Herein, we report a cost-effective methodology to deposit MXene on a conductive graphitic pencil electrode (GPE). MXenes can provide active surface area due to their clever morphology of accordion-like sheets; however, the disposition to stack together limits their potential applications. A task-specific ionic liquid (1-methyl imidazolium acetate) is utilized as a multiplex host material to engineer MXene interface via π-π interactions as well as to act as a selective binding site for biomolecules. The resulting IL-MXene/GPE interface proved to be a highly stable interface owing to good interactions between MXene and IL that inhibited electrode leaching and boosted electron transfer at the electrode-electrolyte interface. It resulted in robust dopamine (DA) oxidation with amplified faradaic response and enhanced sensitivity (9.61 µA µM-1 cm-2) for DA detection. This fabricated sensor demonstrated large linear range (10 µM - 2000 µM), low detection limit (702 nM), high reproducibility, and good selectivity. We anticipate that such platform will pave the way for the development of stable and economically viable MXene-based sensors without sacrificing their inherent properties. Scheme 1 Schematic illustration of the IL-MXene/GPE fabrication and oxidative process towards non-enzymatic dopamine sensor.