RESUMEN
The hyperpolymorphic HLA genes play important roles in disease and transplantation and act as genetic markers of migration and evolution. A panel of 107 B-lymphoblastoid cell lines (B-LCLs) was established in 1987 at the 10th International Histocompatibility Workshop as a resource for the immunogenetics community. These B-LCLs are well characterised and represent diverse ethnicities and HLA haplotypes. Here we have applied Pacific Biosciences' Single Molecule Real-Time (SMRT) DNA sequencing to HLA type 126 B-LCL, including the 107 International HLA and Immunogenetics Workshop (IHIW) cells, to ultra-high resolution. Amplicon sequencing of full-length HLA class I genes (HLA-A, -B and -C) and partial length HLA class II genes (HLA-DRB1, -DQB1 and -DPB1) was performed. We typed a total of 931 HLA alleles, 895 (96%) of which were consistent with the typing in the IPD-IMGT/HLA Database (Release 3.27.0, January 20, 2017), with 595 (64%) typed at a higher resolution. Discrepant types, including novel alleles (n = 10) and changes in zygosity (n = 13), as well as previously unreported types (n = 34) were observed. In addition, patterns of linkage disequilibrium were distinguished by four-field resolution typing of HLA-B and HLA-C. By improving and standardising the HLA typing of these B-LCLs, we have ensured their continued usefulness as a resource for the immunogenetics community in the age of next generation DNA sequencing.
Asunto(s)
Sistemas de Computación , Antígenos HLA/genética , Inmunogenética , Internacionalidad , Análisis de Secuencia de ADN , Imagen Individual de Molécula , Alelos , Línea Celular , Humanos , Desequilibrio de Ligamiento/genéticaRESUMEN
The genomic sequence of the novel HLA-B*44:220 allele identified in a British Caucasoid male.
Asunto(s)
Alelos , Sustitución de Aminoácidos , Antígeno HLA-B44/genética , Polimorfismo de Nucleótido Simple , Secuencia de Bases , Codón , Exones , Expresión Génica , Antígeno HLA-B44/inmunología , Prueba de Histocompatibilidad , Humanos , Masculino , Datos de Secuencia Molecular , Alineación de Secuencia , Reino Unido , Población BlancaRESUMEN
Two disposable condenser humidifiers were evaluated in nine ventilated intensive care patients. The Portex "Humid Vent" delivered end-inspired absolute humidities of 22--26.3 g/m3 at end-inspired temperatures of 27--28.3 degrees C. Corresponding humidities and temperatures with the Servo "Humidifier 150" were higher and were constant, at 27.7--29 g/m3 and 29.3--29.7 degrees C respectively. These disposable devices can be used for humidification in intensive care, but only for patients breathing room air, or on a short term basis.
