"Invisible" pulsation and period-doubling bifurcation of harmonic mode locking in a bidirectional fiber laser.

The harmonic mode-locking (HML) "invisible" pulsation (IP) is reported, here, in a bidirectional passively mode-locked fiber laser (BPMLFL). With the help of dispersive Fourier transform (DFT) technology, it is found that due to the alike nonlinear effects experienced by two pulse trains in HML, their evolution is consistent during the IP. Further, as the increase of pump power, period-doubling bifurcations (PDBs) can be observed based on the IP phenomenon in the HML regime, the PDB path experienced by the HML from steady to chaotic is statistically obtained. Finally, the IP and PDB in the bidirectional laser are reproduced and studied through numerical simulations. The effect of IP on the coherence of solitons is further analyzed. We believe our research results will provide new insights into the study of soliton dynamics in fiber lasers.

Collision and dissociation of soliton molecules triggered by gain perturbation in passively mode-locked fiber laser.

Soliton molecule has the properties that similar to those of matter molecule, which brings great research value. The rich dynamics of soliton molecules depend on the complex interactions between pulses. As one of the important factors, gain dynamics has multi-dimensional effects on the interactions of soliton molecules. We here study the interaction process of soliton molecules under gain perturbation, and find the oscillation and collision behaviors of pulses within soliton molecules induced by gain perturbation, as well as the pulses recombination through interactions between soliton molecules. We believe that the energy change in the cavity and the energy transfer based on the continuous wave component play an important role in the evolution process of the dissociation of soliton molecules into three-pulses bunch and single pulse. These findings reveal the effects of gain on the motion of soliton molecules and provide a basis for exploring the control dynamics of soliton molecules.

Observation of the "invisible" pulsation of soliton molecules in a bidirectional ultrafast fiber laser.

A novel optical soliton dynamics phenomenon, called "invisible" pulsation, has gradually attracted extensive interest in recent years, which can only be identified effectively with the help of real-time spectroscopy technique, i.e., dispersive Fourier transformation (DFT). In this paper, based on a new bidirectional passively mode-locked fiber laser (MLFL), the "invisible" pulsation dynamics of soliton molecules (SMs) is systematically studied. It is indicated that the spectral center intensity, pulse peak power and relative phase of SMs are periodically changed during the "invisible" pulsation, while the temporal separation inside the SMs is constant. The degree of spectral distortion is positively correlated with the pulse peak power, which verifies that self-phase modulation (SPM) is the inducement of spectral distortion. Finally, the universality of the SMs "invisible" pulsation is further experimentally verified. We believe our work is not only conducive to the development of compact and reliable bidirectional ultrafast light sources, but also of great significance to enrich the study of nonlinear dynamics.

Spatiotemporal mode-locked fiber laser based on dual-resonance coupling long-period fiber grating.

Spatiotemporal mode-locked (STML) fiber lasers have become an excellent platform in nonlinear optics research due to the rich nonlinear evolution process. In order to overcome modal walk-off and realize phase locking of different transverse modes, it is usually crucial to reduce the modal group delay difference in the cavity. In this paper, we use long-period fiber grating (LPFG) to compensate the large modal dispersion and differential modal gain in the cavity, realizing the spatiotemporal mode-locking in step-index fibers cavity. The LPFG inscribed in few-mode fiber could induce strong mode coupling, which has wide operation bandwidth based on dual-resonance coupling mechanism. By using dispersive Fourier transform involved intermodal interference, we show that there is a stable phase difference between the transverse modes constituting the spatiotemporal soliton. These results would be beneficial for the study of spatiotemporal mode-locked fiber lasers.

Dynamics of pulsating solitons derived from asymmetrical dispersive waves.

Pulsating soliton (PS) as a local structure of nonlinear systems has induced substantial interest in nonlinear photonics and ultrafast lasers. However, the interaction mechanism between PSs has not been fully studied. Here, the vital role of the asymmetric dispersive wave (DW) in PSs interaction is investigated for the first time. Based on the complex Ginzburg-Landau equation (CQGLE), we find that the asynchronous pulsating soliton molecule (PSM) composed of strong PSs and weak PSs will produce frequency shift due to the asymmetric DW, and the state of the PS can be transferred through the DW during the collision between PSs and PSM. Moreover, we firstly characterize the PS with asymmetric DW in experiment, and observe the drift of PSM, which agree with our simulation that the asymmetric DW can cause the frequency shift of PSMs. Our results provide new insights into the multi soliton interaction of nonlinear systems.

Alterations in immune cell heterogeneities in the brain of aged zebrafish using single-cell resolution.

Immunocytes, including the microglia, are crucial in the neurodegenerative process in old people. However, the understanding of regarding microglia heterogeneity and other involved immunocytes remains elusive. We analyzed 26,456 immunocytes from 12-and 26-month-old zebrafish brains at single-cell resolution. Microglia and T lymphocytes were detected in the brain at both time points. Two types of microglia were annotated, namely, ac+ microglia and xr+ microglia, which were clustered into subsets 1, 2, 3, 4, 5, and subsets 6, 7, 8, 9, respectively. Diversified microglia predominated the adult brains and cooperated with T cells to perform the functions of immune response and neuronal nutrition. We validated the specific microglia markers. The novel transgenic lines, Tg(lgals3bpb:eGFP) and Tg(apoc1:eGFP), were created, which faithfully labeled ac+ microglia and served as valuable labeling tools. However, the microglia population reduced while T cells of six subtypes intriguingly increased to serve as the primary immune cells in aged brains. Unlike in 12-month-old brains, T cells, together with microglia, exhibited a coordinated signature of inflammation in the 26-month-old brains. Our findings revealed the immunocytes atlas in aged zebrafish brains. It implied the involvement of microglia and T cells in the progression of neurodegeneration in aging.

High-order mode erbium-doped fiber laser based on cavity LPFG converters.

We experimentally investigate high-order mode (HOM) generation at a wavelength of 1.5 µm in an all-fiber erbium-doped laser based on a long-period fiber grating (LPFG). The CW laser emission is achieved when the pump power is above the threshold of 10 mW. An LPFG with a 15 dB bandwidth of 147.76 nm from 1502.76 nm to 1650.52 nm is used as a mode converter inside the cavity. The generation of the broadband L P 11 mode is ultimately obtained. By using a few-mode output coupler, we can obtain the intracavity conversion of the linear polarization mode. Single-, dual-, triple-, and quadruple-wavelength operations can be achieved by changing the polarization state of the polarization controllers in the cavity. The tunable range of the output wavelengths is up to ∼23.20n m. The output power and slope efficiency of the HOMs are presented and discussed. We believe our work might benefit the investigation of HOM fiber lasers, and might be further applied to the intracavity conversion of the linear polarization mode or orbital angular momentum beams.

Transition between noise-like pulses and Q-switching in few-mode mode-locked lasers.

Spatiotemporal mode-locked lasers have attracted extensive attention of researchers due to the complex nonlinear evolution process. Compared to single-mode mode-locked lasers, intermodal interactions greatly affect the pulses evolution in spatiotemporal mode-locked lasers. Here, we experimentally investigate the transition process between noise-like pulses and Q-switching pulses in few-mode mode-locked laser by rotating the plates, where a transition state is greatly broadened in the time domain. By means of spectral filtering, we verify that the process is the reconstruction of Q-switching between different modes to noise-like pulses. Furthermore, during the evolution of noise-like pulses, soliton collisions are detected using dispersive Fourier transform technology. Our research contributes to revealing the transient evolution process in few-mode mode-locked lasers, and enriches the study of nonlinear process.

Brain milieu induces early microglial maturation through the BAX-Notch axis.

Microglia are derived from primitive myeloid cells and gain their early identity in the embryonic brains. However, the mechanism by which the brain milieu confers microglial maturation signature remains elusive. Here, we demonstrate that the baxcq55 zebrafish and Baxtm1Sjk mouse embryos exhibit similarly defective early microglial maturation. BAX, a typical pro-apoptotic factor, is highly enriched in neuronal cells and regulates microglial maturation through both pro-apoptotic and non-apoptotic mechanisms. BAX regulates dlb via the CaMKII-CREB axis calcium-dependently in living neurons while ensuring the efficient Notch activation in the immigrated pre-microglia by apoptotic neurons. Notch signaling is conserved in supporting embryonic microglia maturation. Compromised microglial development occurred in the Cx3cr1Cre/+Rbpjfl/fl embryonic mice; however, microglia acquire their appropriate signature when incubated with DLL3 in vitro. Thus, our findings elucidate a BAX-CaMKII-CREB-Notch network triggered by the neuronal milieu in microglial development, which may provide innovative insights for targeting microglia in neuronal disorder treatment.

Nrf2 dictates the neuronal survival and differentiation of embryonic zebrafish harboring compromised alanyl-tRNA synthetase.

tRNA synthetase deficiency leads to unfolded protein responses in neuronal disorders; however, its function in embryonic neurogenesis remains unclear. This study identified an aars1cq71/cq71 mutant zebrafish allele that showed increased neuronal apoptosis and compromised neurogenesis. aars1 transcripts were highly expressed in primary neural progenitor cells, and their aberration resulted in protein overloading and activated Perk. nfe2l2b, a paralog of mammalian Nfe2l2, which encodes Nrf2, is a pivotal executor of Perk signaling that regulates neuronal phenotypes in aars1cq71/cq71 mutants. Interference of nfe2l2b in nfe2l2bΔ1/Δ1 mutants did not affect global larval development. However, aars1cq71/cq71;nfe2l2bΔ1/Δ1 mutant embryos exhibited increased neuronal cell survival and neurogenesis compared with their aars1cq71/cq71 siblings. nfe2l2b was harnessed by Perk at two levels. Its transcript was regulated by Chop, an implementer of Perk. It was also phosphorylated by Perk. Both pathways synergistically assured the nuclear functions of nfe2l2b to control cell survival by targeting p53. Our study extends the understanding of tRNA synthetase in neurogenesis and implies that Nrf2 is a cue to mitigate neurodegenerative pathogenesis.

Elastic and inelastic collision dynamics between soliton molecules and a single soliton.

Dissipative systems form various self-organized states owing to the abundant attractor structures. The study of the response of different self-organized states under collision perturbation is of great significance for understanding the dissipative nonlinear systems. The collision dynamics of single soliton and soliton molecules can not only assist the stability analysis of attractors, but also reveal the rich physical connotations of soliton interactions. Here, for the first time, the collision processes of single soliton and soliton molecules in different excited states are detected using the dispersive Fourier transform technology. The collision processes include the disintegration and rebuilding of soliton molecules as well as chaotic oscillating evolution, accompanied by the emergence of transition states such as triple binding state, soliton fusion and acceleration. According to whether the soliton molecule can return to its initial excited state, the collisions are classified as elastic and inelastic. The different interaction strength between solitons is an important condition for rebuilding stable soliton molecules. Numerical simulations show that the gain dynamics are the main physical origin of collisions. Our research will stimulate in-depth research on the interaction of self-organized states in nonlinear systems such as chemical molecules, and have potential applications in optical logic gates.

The CXCR4-CXCL12 axis promotes T cell reconstitution via efficient hematopoietic immigration.

T cells play a critical role in immunity to protect against pathogens and malignant cells. T cell immunodeficiency is detrimental, especially when T cell perturbation occurs during severe infection, irradiation, chemotherapy, and age-related thymic atrophy. Therefore, strategies that enhance T cell reconstitution provide considerable benefit and warrant intensive investigation. Here, we report the construction of a T cell ablation model in Tg(coro1a:DenNTR) zebrafish via metronidazole administration. The nascent T cells are mainly derived from the hematopoietic cells migrated from the kidney, the functional homolog of bone marrow and the complete recovery time is 6.5 days post-treatment. The cxcr4b gene is upregulated in the responsive hematopoietic cells. Functional interference of CXCR4 via both genetic and chemical manipulations does not greatly affect T lymphopoiesis, but delays T cell regeneration by disrupting hematopoietic migration. In contrast, cxcr4b accelerates the replenishment of hematopoietic cells in the thymus. Consistently, Cxcl12b, a ligand of Cxcr4, is increased in the thymic epithelial cells of the injured animals. Decreased or increased expression of Cxcl12b results in compromised or accelerated T cell recovery, respectively, similar to those observed with Cxcr4b. Taken together, our study reveals a role of CXCR4-CXCL12 signaling in promoting T cell recovery and provides a promising target for the treatment of immunodeficiency due to T cell injury.

Oscillatory self-organization dynamics between soliton molecules induced by gain fluctuation.

In passively mode-locked fiber lasers (PMLFLs), the dissipative solitons (DSs) can self-organize to form complex structures through delicate interactions. However, it is still elusive to control these soliton structures by external influences. We here find that at a certain critical power, the location between two soliton molecules can be controlled by a slow modulated pump power. After applying the pump power with periodic fluctuation, two soliton molecules oscillate from the state of soliton molecular complex to stable distribution with maximum inter-molecular separation. During this process, the internal structure of each soliton molecule keeps steady. The slow gain depletion and recovery mechanism which plays a dominant role affects the motion of soliton molecules. These results could further expand the molecular analogy of spectroscopy and stimulate the development of optical information storage and processing.

Single-resonator, stable dual-longitudinal-mode optofluidic microcavity laser based on a hollow-core microstructured optical fiber.

A single-resonator, stable dual-longitudinal-mode optofluidic microcavity laser based on a hollow-core microstructured optical fiber is proposed and experimentally demonstrated. The resonator and microfluidic channel are integrated in the hollow-core region of the fiber, inside which a hexagonal silica ring is used as the only resonator of the laser. Experimental results show that with mixing a small amount of Rhodamine B into a 1 mM Rhodamine 6G solution to form a dual-dye solution as a gain medium, the laser obtained by the method of lateral pumping can operate at dual longitudinal modes, with a threshold of 90 nJ/mm2. By adjusting the concentration of Rhodamine B, the lasing wavelength of the laser and the power ratio of the two wavelengths can be controlled. And because the laser emission is co-excited by different kinds of dye molecules, the mode competition is diminished, enabling the simultaneously efficient optical gain and therefore lasing at dual longitudinal modes stably with a maximum lasing intensity fluctuation of 3.2% within 30 minutes even if the dual longitudinal modes have the same linear polarization states. This work can open up promising opportunities for diverse applications in biosensing and medical diagnosis with high sensitivity and integrated photonics with compact structure.

Real-time observation of multi-soliton asynchronous pulsations in an L-band dissipative soliton fiber laser.

Based on the experimental platform of an L-band normal-dispersion mode-locked fiber laser, we report the first observation, to our knowledge, of three novel types of multi-soliton asynchronous pulsation phenomena by virtue of the dispersive Fourier transform technique. The experimental results provide new insights into the complex multi-soliton dynamics under unstable mode-locking conditions. It is confirmed that more than one pulsating solution can coexist in a multi-pulse situation and that each soliton may evolve periodically in different ways. This implies that subsequent experimental and theoretical studies on multi-soliton need to take the differences among pulses into account and retrieve more degrees of freedom.

Real-time observation of dissociation dynamics within a pulsating soliton molecule.

Real-time detection of the ultrafast dynamics in complex nonlinear optical systems provides novel insights into pulse interactions and dynamic patterns, especially for soliton molecules. Herein, the concept of soliton molecule is extended to the pulsating regime, revealing the dynamical diversity of soliton molecule and the universality of pulsating behavior. By virtue of the dispersive Fourier transform (DFT) technique, we present the first experimental observation of the dissociation dynamics within a pulsating soliton molecule generated in an L-band normal-dispersion mode-locked fiber laser. The results provide valuable references for resolving the interactions in complex dissipative systems.

Transient behaviors of pure soliton pulsations and soliton explosion in an L-band normal-dispersion mode-locked fiber laser.

As one of the most striking localized structures in dissipative systems, pulsating soliton has been widely studied in theory but rarely observed in experiments. Here, three typical types of soliton pulsations are experimentally demonstrated in an L-band normal-dispersion mode-locked fiber laser via the dispersive Fourier transform (DFT) technique. According to the distinctive features, they are classified as single-periodic pulsating soliton, double-periodic pulsating soliton and soliton explosion. These pulsations have common features such as energy oscillation, bandwidth breathing and temporal shift. However, the pulse is repeated every two oscillations for double-periodic pulsating soliton. When it comes to soliton explosion, because of the intermittent overdriven nonlinear effect induced by the extreme energy oscillation, the spectrum cracks into pieces at a periodic manner. To the best of our knowledge, it is the first time that both pure soliton pulsations and soliton explosion are observed experimentally in the same fiber laser. The results will enhance a more comprehensive understanding for the soliton pulsating phenomena.

Dynamic evolution of pulsating solitons in a dissipative system with the gain saturation effect.

We numerically investigate the dynamic evolution of pulsating solitons based on complex cubic-quintic Ginzburg-Landau equation with gain dynamics effects. We show that an additional soliton can be generated by the disturbance caused by a dispersion wave emitted by a single-period pulsating soliton and these solitons form soliton molecule. More complicated oscillating processes, such as snaking pulsation and double-periodic pulsation are actuated by periodic collision of the entangled solitons. Moreover, the dispersive wave, caused by high gain parameters and the soliton collision, appears periodically which is in sync with the pulsating process. These results are consistent with the recent experiments of soliton pulsations measured by dispersive Fourier transform techniques, and will stimulate further experimental research of the complex multi-soliton bunches in dissipative systems.