BCp12/PLA combination: A novel antibacterial agent targeting Mur family, DNA gyrase and DHFR.

The combination of natural antimicrobial peptide BCp12/phenyllatic acid (BCp12/PLA) presents a more efficient antibacterial effect, but its antibacterial mechanism remains unclear. This study studied the synergistic antibacterial mechanism of BCp12 and PLA against S. aureus. The results demonstrated that the BCp12/PLA combination presented a synergistic antibacterial effect against S. aureus, with a fractional inhibitory concentration of 0.05. Furthermore, flow cytometry and scanning electron microscope analysis revealed that BCp12 and PLA synergistically promoted cell membrane disruption compared with the group treated only with one compound, inducing structural cell damage and cytoplasmic leakage. In addition, fluorescence spectroscopy analysis suggested that BCp12 and PLA synergistically influenced genomic DNA. BCp12 and PLA targeted enzymes related to peptidoglycan and DNA synthesis and interacted by hydrogen bonding and hydrophobic interactions with mur enzymes (murC, murD, murE, murF, and murG), dihydrofolate reductase, and DNA gyrase. Additionally, the combined treatment successfully inhibited microbial reproduction in the storage of pasteurized milk, indicating that the combination of BCp12 and PLA can be used as a new preservative strategy in food systems. Overall, this study could provide potential strategies for preventing and controlling foodborne pathogens.

High-Q Thin-Film Lithium Niobate Microrings Fabricated with Wet Etching.

Thin-film lithium niobate (TFLN) has been widely used in electro-optic modulators, acoustic--optic modulators, electro-optic frequency combs, and nonlinear wavelength converters owing to the excellent optical properties of lithium niobate. The performance of these devices is highly dependent on the fabrication quality of TFLN. Although state-of-the-art TFLN microrings with an intrinsic quality factor (Q-factor) exceeding 1 × 107 have been realized by inductively coupled plasma-reactive ion etching (ICP-RIE) and chemical mechanical polishing (CMP), ICP-RIE has moderate throughput, moderate reproducibility, and high cost in etching TFLN, while CMP features moderate throughput and low cost in etching TFLN. Here, a wet etching method for TFLN, leading to the fabrication of a micro-racetrack with an intrinsic Q-factor of over 9.27 × 106 is developed. The suitability of this method to fabricate a narrow coupling gap between the bus waveguide and microring enables the coupling conditions of the microring to be customized. This method features a high throughput, a high reproducibility, and a low cost in etching TFLN, showing the potential to boost the mass production of integrated LN photonic devices with high fidelity and affordability dramatically.

Design of on-chip mid-IR frequency comb with ultra-low power pump in near-IR.

Broadband mid-infrared frequency combs are of particular interest to mid-infrared spectroscopy due to their ruler-like precise discrete comb teeth. However, the state-of-the-art mid-infrared frequency combs are usually limited to low integration level and high pump power as a result of the conventional way of mid-infrared frequency comb generation--producing a near-infrared frequency comb first and then convert it to mid-infrared regime through a nonlinear process. Here, we theoretically investigate two integrated designs for generating mid-infrared frequency combs with ultra-low power pump based on the lithium-niobate on insulator (LNOI) platform. Utilizing periodically poled lithium-niobate (PPLN) waveguides and microring electro-optic phase modulators, we switch the conventional order of comb generation and nonlinear conversion. This paradigm shift significantly improves the conversion efficiency of mid-infrared frequency comb generation and obviates the need for femtosecond lasers. Our theoretical results predict that a broadband mid-infrared frequency comb around 4.3 µm with nanowatt-power-level comb teeth can be produced from continuous-wave (CW) inputs whose power is lower than 5 mW with an ultra-high conversion efficiency above 1800 %/W. Our designs of mid-infrared frequency comb have high controllability, flexibility and integration level, enabling the miniaturization of mid-infrared spectrometers.