Bimetallic FeCo-MOFs mediated Au nanorods etching for the multi-colorimetric and photothermal immunosensing of illegal additive.

With the rapid expansion of the health food industry, the scope of safety supervision has also increased. However, traditional instrument detection methods cannot meet the requirements for the rapid on-site detection. Hence, the development of a rapid, precise, and simple method for the analysis of illegal additives in health foods is of great importance. In this work, by using FeCo-MOFs as mimetic peroxidase to mediate Au nanorods (Au NRs) etching, a dual-mode immunosensor based on multi-colorimetric and photothermal signals was fabricated to detect furosemide (FUR). In multi-colorimetric channel, the localized surface plasmon resonance (LSPR) peaks of Au NRs shifted blue, resulting in multi-color changes from red to gray to blue and finally to purple. In photothermal channel, the photothermal effect of Au NRs decreased, resulting in temperature changes. In the range of 1.0 × 10-5-1.0 × 10-2 µg/mL, both LSPR peak blue shift and temperature changes were linearly correlated with the logarithm of FUR concentration, with the detection limits were 4.9 × 10-6 and 8.5 × 10-6 µg/mL, respectively. Furthermore, its concentration can be accurately and intuitively assessed through the observation of vivid colorimetric changes. This advancement offers a highly promising approach for the on-site detection of FUR, facilitating timely and efficient monitoring, thereby significantly enhancing regulatory compliance and ensuring consumer safety.

One-step strategy for cartilage repair using acellular bone matrix scaffold based in situ tissue engineering technique in a preclinical minipig model.

Cartilage defects are most commonly seen in the knee joint. However, due to the limited self-recovery ability of cartilage, the repair of articular cartilage defects is still a great challenge despite that various approaches have been proposed. We designed a strategy to induce cartilage repair using acellular bone matrix (ABM), thereby creating an appropriate microenvironment for the in-situ cells with an easy surgical application. An in vitro system demonstrated that the ABM scaffold could promote cell adhesion, growth, proliferation, and chondrogenesis of mesenchymal stem cells. This experiment was performed in a minipig cartilage repair model. The repaired tissue was hyaline-like cartilage according to the morphological and histological results. The mechanical properties of the repaired tissue were similar to those of normal cartilage. The integration of repaired tissue and normal tissue in the ABM+M group was better than those of other two groups. The ABM-based, one-stage, minimally invasive, in situ procedure for cartilage regeneration can potentially improve the treatment of articular cartilage defects.

The diagnostic value of magnetic resonance imaging for different types of subscapularis lesions.

PURPOSE: To investigate the accuracy of preoperative 1.5 T magnetic resonance imaging (MRI) interpreted with standardized procedure for diagnosing subscapularis (SSC) tears identified by arthroscopy. The diagnostic accuracy of MRI for different types of SSC lesions was also evaluated. METHODS: Two-hundred and seventy-two consecutive patients with rotator cuff tears identified by arthroscopy were included into this study. All patients had preoperative MRI scans with 1.5 T strength. Totally, seven different signs of SSC tears were evaluated on MRI scans. The diagnostic value of MRI was evaluated, respectively, according to two classifications of SSC lesions. The definitive diagnosis for SSC lesions was based on the arthroscopic findings. RESULTS: Among the 272 patients in this study, 107 (39 %) had SSC tears confirmed by arthroscopy. The surgeons correctly diagnosed 88 of 107 patients with SSC tears. The overall sensitivity was 82.2 %. The sensitivity of MRI for types I, II, III, IV and V was 70, 82.4, 96, 100 and 100 %, respectively, based on the classification by Lafosse. The sensitivity of MRI for partial-thickness and partial-width, full-thickness and partial-width, full-thickness and full-width tear was 75.3, 96.2, 100 %, respectively, according to the classification by Kim. CONCLUSIONS: The sensitivity of MRI in predicting SSC tears preoperatively was improved with the current procedure. Understanding of certain MRI characteristics of SSC tears could increase the accuracy for diagnosing SSC lesions. 1.5 T MRI of the SSC was not reliable for predicting partial thickness and especially less than 1/3 width tears. LEVEL OF EVIDENCE: III.

One-step repair for cartilage defects in a rabbit model: a technique combining the perforated decalcified cortical-cancellous bone matrix scaffold with microfracture.

BACKGROUND: Cartilage repair still presents a challenge to clinicians and researchers alike. A more effective, simpler procedure that can produce hyaline-like cartilage is needed for articular cartilage repair. HYPOTHESIS: A technique combining microfracture with a biomaterial scaffold of perforated decalcified cortical-cancellous bone matrix (DCCBM; composed of cortical and cancellous parts) would create a 1-step procedure for hyaline-like cartilage repair. STUDY DESIGN: Controlled laboratory study. METHODS: For the in vitro portion of this study, mesenchymal stem cells (MSCs) were isolated from bone marrow aspirates of New Zealand White rabbits. Scanning electron microscopy (SEM), confocal microscopy, and 1,9-dimethylmethylene blue assay were used to assess the attachment, proliferation, and cartilage matrix production of MSCs grown on a DCCBM scaffold. For the in vivo experiment, full-thickness defects were produced in the articular cartilage of the trochlear groove of 45 New Zealand White rabbits, and the rabbits were then assigned to 1 of 3 treatment groups: perforated DCCBM combined with microfracture (DCCBM+M group), perforated DCCBM alone (DCCBM group), and microfracture alone (M group). Five rabbits in each group were sacrificed at 6, 12, or 24 weeks after the operation, and the repair tissues were analyzed by histological examination, assessment of matrix staining, SEM, and nanoindentation of biomechanical properties. RESULTS: The DCCBM+M group showed hyaline-like articular cartilage repair, and the repair tissues appeared to have better matrix staining and revealed biomechanical properties close to those of the normal cartilage. Compared with the DCCBM+M group, there was unsatisfactory repair tissues with less matrix staining in the DCCBM group and no matrix staining in the M group, as well as poor integration with normal cartilage and poor biomechanical properties. CONCLUSION: The DCCBM scaffold is suitable for MSC growth and hyaline-like cartilage repair induction when combined with microfracture. CLINICAL RELEVANCE: Microfracture combined with a DCCBM scaffold is a promising method that can be performed and adopted into clinical treatment for articular cartilage injuries.

[Progress in leaf area index retrieval based on hyperspectral remote sensing and retrieval models].

The leaf area index (LAI) is a very important parameter affecting land-atmosphere exchanges in land-surface processes; LAI is one of the basic feature parameters of canopy structure, and one of the most important biophysical parameters for modeling ecosystem processes such as carbon and water fluxes. Remote sensing provides the only feasible option for mapping LAI continuously over landscapes, but existing methodologies have significant limitations. To detect LAI accurately and quickly is one of tasks in the ecological and agricultural crop yield estimation study, etc. Emerging hyperspectral remote sensing sensor and techniques can complement existing ground-based measurement of LAI. Spatially explicit measurements of LAI extracted from hyperspectral remotely sensed data are component necessary for simulation of ecological variables and processes. This paper firstly summarized LAI retrieval method based on different level hyperspectral remote sensing platform (i. e., airborne, satelliteborne and ground-based); and secondly different kinds of retrieval model were summed up both at home and abroad in recent years by using hyperspectral remote sensing data; and finally the direction of future development of LAI remote sensing inversion was analyzed.

Twelve cases of the pectoralis major muscle tendon rupture with surgical treatment-an average of 6.7-year follow-up.

BACKGROUND: Pectoralis major tendon rupture is a relatively rare injury leading to both functional and cosmetic deficiency. The peak torque of the pectoralis major is significantly decreased after conservational treatment of a total rupture. We suppose that surgical intervention is better choice. METHODS: We retrospectively evaluated 12 patients who were diagnosed with distal pectoralis major muscle rupture and underwent operation in our institute from 1993 to 2007. All patients were male with a mean age of 32 (19 - 54) years. In 4 patients, tendon reconstruction was accomplished by fixing the tendon to the humerus using sutures passed through predrilled humeral bone tunnels. In 8 patients, tendon reconstruction was accomplished by directly suturing the muscle to the tendon. All patients followed an accelerated rehabilitation protocol. Treatment results were evaluated according to the following criteria: (1) visual analogue pain scale, (2) isokinetic strength measurements, (3) range of motion of shoulder joint, (4) cosmetic result, and (5) postoperative sports activity performance. In this study, we aimed to describe our surgical technique of reconstruction of the rupture of pectoralis major muscle and to summarize the clinical results of the operative treatment. RESULTS: At the final follow-up examination (6.5 years postoperatively), only nine patients were available for evaluation. Three of them had excellent results, and five had good results, while one had poor results. Eight of the patients were able to return to their preinjury level of sporting activity. In summary, 89% of the patients achieved excellent or good results. CONCLUSION: Surgical treatment by anatomic tendon repair and accelerated rehabilitation can make recovery of strength and function of the pectoralis major muscle.

How to avoid the risk of intraoperative cartilage damage in anatomic four tunnel double bundle anterior cruciate ligament reconstruction.

Double bundle (DB) anterior cruciate ligament (ACL) reconstruction is technically demanding. In order to create four anatomical anteromedial (AM) and posterolateral (PL) bone tunnels many surgeons adopt new ways of tibial and femoral bone tunnel drilling. From surgical experience, these technical changes might increase the risk for intraoperative pitfalls. An intraoperative articular cartilage damage to the medial femoral condyle or the medial tibial plateau could be disastrous for the patient. It may be caused by an insufficient anteromedial portal technique for femoral AM and PL bone tunnel drilling or flat tibial AM or PL bone tunnel reaming. Potential pitfalls may be avoided by small modifications to the surgical technique. In this present technical note, a sequence of surgical steps are described, which may help to avoid articular cartilage damage to the medial femoral condyle and medial tibial plateau in anatomical four tunnel DB ACL reconstruction.

Medial plica after reconstruction of anterior cruciate ligament.

BACKGROUND: The medial plica may be caused by direct trauma or joint degeneration, which also could be iatrogenic. There have been few reports in the literature discussing incidence of the medial plica caused by an operation on the knee joint, specifically after the reconstruction of anterior cruciate ligament (ACL). In this study, we aimed to evaluate and analyze the relationship between the incidence of the medial plica and reconstruction of the ACL. METHODS: A retrospective case series study was conducted to review the findings of 1085 patients between 2003 and 2007, who underwent second-look arthroscopy after reconstruction of the ACL (between 2002 and 2006). The correlation of the incidence of medial plica with the stability of the knee joint, the time from onset of injury to reconstruction surgery, the associated injuries, and the rate of progress during postoperative rehabilitation were analyzed. RESULTS: We found that 722 patients had the structure of a medial plica. The incidence after reconstruction of the anterior cruciate ligament (66.5%) was significantly higher than usually reported. All these medial plica had avascular fibrotic and thickened edges. An excision of pathologic medial plica and fat pad synovial fringes were done. The incidences were significantly different between the two groups with their reconstruction operation time, from onset of injury to surgery (less than one month or over 2 years), and the progress rate of postoperative rehabilitation (knee flexion could not be over 90 degrees in four weeks). The incidence was not different between the groups with knee stable conditions. CONCLUSIONS: Medial plica is more common in patients after reconstruction of ACL. More associated injuries and more rehabilitation difficulties can increase the medial plica incidence.

[Corrosiveness of Ti-Fe-Mo-Mn-Nb-Zr alloys in various pH lactic acids].

Variation in pH value of oral cavity circumstance causes decayed tooth. In this paper, corrosions of Ti-Fe-Mo-Mn-Nb-Zr dental alloys in various pH lactic acids were studied by immersion test. In the case of pH=4, which is an acidity slightly larger than that causes decayed tooth, Ti-Fe-Mo-Mn-Nb-Zr alloys are entirely corrosion-resistant, and the corrosion type is pitting. With the increase in acidity, the corrosion-resistance of Ti-Fe-Mo-Mn-Nb-Zr alloys deteriorates, and the corrosion type changes from pitting to intergranular corrosion. Fe ion is the most dissolved substance in impregnation, which means Fe goes against the corrosion-resistance of Ti alloys. Both results of thermodynamics calculation and X-ray photoelectron spectroscopy have shown that Mn2O3, Nb2O5 and TiO2 form oxide film on the surface of Ti-Fe-Mo-Mn-Nb-Zr alloys.

Effects of Ce on the short-term biocompatibility of Ti-Fe-Mo-Mn-Nb-Zr alloy for dental materials.

Effects of Ce on the short-term biocompatibility of Ti-Fe-Mo-Mn-Nb-Zr alloy designed for implant materials were studied by acute toxicity test, hemolytic test, and MTT assay. The elements and their concentration in surface films and extraction media of Ti alloys were investigated with XPS and ICP, respectively. The primary compositions of the surface films of Ti alloys with 0.3% Ce and without Ce were TiO2 and Nb2O5. There were 0.2 mg/l Fe and 0.16 mg/l Mn in the extraction medium of Ti alloy without Ce, while 0.27 mg/l Fe and 0.87 mg/l Mn in the extraction medium of Ti alloy with 0.3% Ce. The concentrations of Fe and Mn in the medium were too low to have any significant effects on human health. There was no sign of cytotoxicity in these tests. The cytotoxicity levels of Ti alloys without Ce and with 0.3% Ce were graded 0 and 1, respectively. The hemolytic degrees of Ti alloys without Ce and with 0.3% Ce were 0.558% and 0.67%, respectively. The cells being incubated in the extraction medium were normal. These phenomena indicated that Ce was innocuous within the concentration range of this study. In addition, the hemolytic ratio and toxicity level of Ti alloy with 0.3% Ce were a little higher than that of Ti alloy without Ce. This meant that Ce would slightly increase the toxicity of Ti alloy.

[Evaluation on biocompatibility of Ti-Fe-Mo-Mn-Nb-Zr alloy].

In this study, the general toxicity tests including acute toxicity test, haemolysis test, MTT assay of Ti-Fe-Mo-Mn-Nb-Zr alloys were carried out. The morphology of these cells was also observed under phase-contrast microscope. By using X-ray photoelectron spectroscopy(XPS), the kind and mol% of element in surface film were studied. The kind and concentration of element in dipping fluid were investigated by ICP atomic emission spectrometry. The results showed the primary component is TiO2 in surface film. The dipping fluid of Ti-Fe-Mo-Mn-Nb-Zr alloys contains Fe 0.2-1.07 mg/l and Mn 0.16-0.5 mg/l; such dental materials are beneficial to health. No cytotoxic effect was disclosed by in vitro and in vivo tests. The level of cytotoxicity was grade 0 and 1; the haemolysis degree was 0.558%-0.642%, i.e. less than 5%. The cells growing in the extract showed normal morphology. These data indicate that Ti-Fe-Mo-Mn-Nb-Zr alloy, as a dental material, has good biocompatibility.

[The influence of Ce on microstructures and mechanics performances of Ti-Fe-Mo-Mn-Nb-Zr alloys].

The influences of Ce on the microstructure and mechanics performances of Ti-Fe-Mo-Mn-Nb-Zr alloys were studied and presented in this paper. The microstructure of no-Ce Ti-Fe-Mo-Mn-Nb-Zr alloy was thick, long and dendritic, and the microstructure of alloy was fined by Ce. With the increase in Ce wt%, the microstructure became equiaxial gradually. Hardness decreased with the increase in Ce wt%. The compression yield strength became greater with the increasing of Ce wt%, but after the Ce wt% increased to a certian degree, the compression yield strength became smaller with the further increasing of Ce wt%. This phenomenon was explained in the light of bond energy and X-ray diffraction experiment. X-ray diffraction experiment showed that the alloys were beta-Ti.