RESUMEN
Catecholamines serve as a neuromodulators of many behavioral and endocrine responses in different vertebrates including amphibians. However, the neuroanatomical studies on catecholamines, especially in the tadpole brain are limited. In this study, we report the distribution of catecholaminergic neurons in different areas of the brain in the tadpole of Microhyla ornata at metamorphic climax stage. Application of antisera against tyrosine hydroxylase (TH) revealed the presence of catecholaminergic cells and fibres in the olfactory bulb, the telencephalon, the diencephalon, the mesencephalon, the spinal cord and the pituitary gland. Whereas densest aggregations of TH-immunoreactive (TH-ir) fibres were noticed in the nucleus accumbens and the amygdala pars medialis regions of the telencephalon, highest population of TH-ir cells with dorsolaterally and rostrocaudally oriented fibres was observed in the preoptic area. Larger and distinct TH-ir cell bodies along with few dorsolaterally oriented TH-ir fibres were scattered throughout the suprachiasmatic nucleus. While moderate to intensely stained clusters of TH-ir cells were observed in dorsal and ventral hypothalamic regions, conspicuous TH-ir cells and fibres were seen in the pars distalis of the pituitary gland. In the nucleus tuberculi posterioris, numerous moderate sized TH-ir cells were found along the margin of the third ventricle and the fibres from this region were oriented dorsolaterally towards the torus semicircularis and tectal regions, whereas well organized largest TH-ir cells and fibres were seen in the tegmentum. In the spinal cord, medium sized TH-ir cells along with numerous laterally running fibres were encountered. Overall, widespread distribution of the TH-ir cells and fibres in the brain and the pituitary gland of the tadpole suggest diverse roles for the catecholamines in regulation of locomotion, olfaction, skin pigmentation and endocrine responses during final stages of metamorphosis in M. ornata.