Modulation of toxic effects of ammonia on growth, pathology of liver and kidney tissues and relative expression of GH and IGF-1 Genes by CoQ10 Supplementation in Oncorhynchus mykiss.

Reducing the negative impact of environmental and stressful factors is a crucial step in achieving sustainable aquaculture. Therefore, a study was aimed at evaluating the impacts of Coenzyme Q10 (CoQ10) supplementation on growth, relative gene expression of Growth Hormone (GH) and Insulin-like growth factor-1 (IGF-1), liver and kidney histopathology against stress induced by ammonia in Rainbow trout (Oncorhynchus mykiss). The fish were given feed containing different levels of CoQ10 for 8 weeks: Control - CoQ10 0%, G1 - CoQ10 0.1%, G2 - CoQ10 0.5% and G3 - CoQ10 1%. At the end of the experiment, fish were exposed to ammonia stress concentration at 0.6mg/L for 24 h to assess liver and kidney tissue damage. Results showed that there was a significant activity increase in GH and IGF-1 genes due to supplementation with CoQ10 alone (p < 0.05). Gene expression for GH increased about two-fold whereas that for IGF-1 experienced a four-fold upregulation compared to controls (p < 0.05). CoQ10's-related antioxidant effects probably minimized liver and kidney cellular injuries, as significant decreases were observed in ammonia-induced mortality (p < 0.05). In summary, adding CoQ10 to the diet is a potential way to improve fish production through controlling the gene expression of GH and IGF-1, as well as making fish populations more resistant to possible future stress caused by ammonia in intensive or super-intensive aquaculture systems.

Copper-oxide nanoparticles effects on goldfish (Carassius auratus): Lethal toxicity, haematological, and biochemical effects.

The advancement of nanotechnology and the widespread use of nanoparticles (NPs) in various industries have highlighted the importance of studying the potential harmful effects of nanomaterials on organisms. This study aimed to evaluate the lethal toxicity thresholds of Copper Oxide Nanoparticles (CuO-NPs). The investigation focused on examining the sub-lethal toxicity effects of CuO-NPs on blood parameters, as well as their influence on the gill tissue and liver of goldfish (Carassius auratus). Goldfish were exposed to varying concentrations of CuO-NPs (10, 20, 30, 40, 60, 80, and 100 mg/L) for 96 h. The Probit software was employed to determine the LC50 (lethal concentration causing 50% fish mortality) by monitoring and documenting fish deaths at 24, 48, 72, and 96-hour intervals. Subsequently, sub-lethal concentrations of 5% LC50 (T1), 10% LC50 (T2), and 15% LC50 (T3) of CuO-NPs were administered based on the LC50 level to investigate their effects on haematological parameters, encompassing the number of red blood cells and white blood cells, hematocrit and haemoglobin levels, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. Additionally, histopathological examinations were conducted on the gill and liver tissues of the studied fish. Results indicated concentration-response of fish mortalities. In general, changes in the blood biochemical parameters of fish exposed to sub-lethal concentrations of CuO-NPs included a significant decrease in leukocyte count and glucose level and an increase in protein and triglyceride levels. Furthermore, an escalation in tissue damage such as gill apical and basal hyperplasia, lamellae attachment, squamous cell swelling, blood cell infiltration, and cellular oedema in gills tissue. and bleeding, increased sinusoidal space, necrosis, lateralization of the nucleus, cell swelling, and water retention in the liver. The findings showed dose-dependent increasing toxicity in goldfish specimens exposed to CuO-NPs.

Solvent-thermal approach of MIL-100(Fe)/Cygnea/Fe3O4/TiO2 nanocomposite for the treatment of lead from oil refinery wastewater (ORW) under UVA light.

The main purpose of this research endeavor is to reduce lead concentrations in the wastewater of an oil refinery through the utilization of a material composed of oyster shell waste (MIL-100(Fe)/Cygnea/Fe3O4/TiO2. Initially, iron oxide nanoparticles (Fe3O4) were synthesized via solvent-thermal synthesis. It was subsequently coated layer by layer with the organic-metallic framework MIL-100 (Fe) using the core-shell method. Additionally, the solvent-thermal method was utilized to integrate TiO2 nanoparticles into the magnetic organic-metallic framework's structure. Varieties of analytical analysis were utilized to investigate the physical and chemical properties of the synthetic final photocatalyst. Nitrogen adsorption and desorption technique (BET), scanning electron microscopy (SEM), scanning electron diffraction pattern (XRD), and transmission electron microscopy (TEM). Following the characterization of the final photocatalyst, the physical and chemical properties of the nanoparticles synthesized in each step, several primary factors that significantly affect the removal efficiency in the advanced oxidation system (AOPs) were examined. These variables consist of pH, photocatalyst dosage, lead concentration, and reaction temperature. The synthetic photocatalyst showed optimal performance in the removal of lead from petroleum wastewater under the following conditions: 35 °C temperature, pH of 3, 0.04 g/l photocatalyst dosage, and 100 mg/l wastewater concentration. Additionally, the photocatalyst maintained a significant level of reusability after undergoing five cycles. The findings of the study revealed that the photocatalyst dosage and pH were the most influential factors in the effectiveness of lead removal. According to optimal conditions, lead removal reached a maximum of 96%. The results of this investigation showed that the synthetic photocatalyst, when exposed to UVA light, exhibited an extraordinary capacity for lead removal.

Fractionation of the Caspian sand goby epidermal exudates using membrane ultrafiltration and reversed-phase chromatography: an investigation on bioactivities.

Bioactive peptide-based drugs have gained exceeding attention as promising treatments for infectious and oxidative-stress-related diseases, are exacerbated by the advent and spread of various multidrug-resistant bacteria and industrial lifestyles. Fish skin mucus has been recognized as a potential source of bioactive peptides, providing the first line of fish defense against invading pathogens which are targeted here to be explored as a new source of biopharmaceutics. Peptide fractions were isolated from the epidermal exudates of Caspian sand goby, Neogobius fluviatilis pallasi, by solid-phase extraction (SPE), ultrafiltration, and reversed-phase chromatography. The resulting fractions were characterized for their antibacterial and antioxidant properties, and results showed that the molecular weight fraction < 5 kDa represented the highest (p < 0.05) bacterial inhibition activity against Staphylococcus aureus and Bacillus subtilis as well as scavenging activity against DPPH and ABTS radicals. Overall, these results introduce the epidermal mucus of Caspian sand goby as a valuable source of bioactive compounds that can be considered new and efficient biopharmaceutics.

Capability of TiO2 and Fe3O4 nanoparticles loaded onto Algae (Scendesmus sp.) as a novel bio-magnetic photocatalyst to degration of Red195 dye in the sonophotocatalytic treatment process under ultrasonic/UVA irradiation.

In this study, the magnetic photocatalyst Scendesmus/Fe3O4/TiO2 was synthesized, and its sonophotocatalytic properties in relation to the degradation of the Red195 dye were evaluated. Particles were characterized using a scanning electron microscope (SEM), Fourier's transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), and a vibrating-sample magnetometer (VSM). At a pH of 5, a photocatalyst dosage of 100 mg, an initial R195 concentration of 100 mg/l, an ultrasound power of 38W, and an exposure time of 20 min, the maximum Red195 removal efficiency (100%) was achieved. After five cycles of recycling, the composite's sonophotocatalytic degradation stability for R195 remains above 95%. Experiments on scavenging indicate that electrons (h+) and hydroxyls (OH-) are indispensable decomposition agents. The removal of R195 by Scendesmus/Fe3O4/TiO2 is consistent with the pseudo-first-order kinetic, Freundlich, and Henderson's isotherm models, as determined by kinetic and isotherm investigations. The negative activation enthalpy of the standard (ΔH°) illuminates the exothermic adsorption mechanism. The increase in standard Gibbs activation free energy (ΔG°) with increasing temperature reveals the process is not spontaneous. As indicated by the negative value of the standard entropy of activation (ΔS°), activation of the reactants resulted in a loss of freedom.

Modulation of toxicity effects of CuSO4 by sulfated polysaccharides extracted from brown algae (Sargassum tenerrimum) in Danio rerio as a model.

Copper is widely used in agriculture and aquaculture due to its high disinfection properties and relatively low cost. However, the increase in copper concentration due to evaporation can lead to water reservoir pollution, which can harm the health of consumers. The present study aimed to determine the role of sulfated polysaccharides (SPs) extracted from Sargassum tenerimum algae in reducing lesions caused by the heavy metal copper. Zebrafish (Danio rerio) were used as a human model in five treatments. The negative and positive control groups were fed a diet containing zero percent of SPs, while the experimental groups were fed 0.5%, 1%, and 1.5% of SPs in three treatments for 56 days, finally CuSO4 was exposed only to the positive control group and the groups fed with SPs. Results showed a significant decrease in the activity level of ALT enzymes (39-16 U/mL), AST (67-46 U/mL), and ALP (485-237 U/mL), confirming the results obtained from histopathological studies in CuSO4 exposed groups. The addition of SPs to the diet resulted in a significant reduction (sig < 0.05) of mortalities due to the decrease of tissue damage. Additionally, due to the anti-inflammatory properties and the protective effect of SPs, a significant decrease (sig < 0.05) was observed in the relative expression of Il-1ß and Tnf-α genes.

Ecological environment monitoring for sustainable development goals in Gomishan international wetland, Gorgan Bay, Caspian Sea.

Monitoring of coastal ecosystems is of particular importance for sustainable development and environmental management. Remote sensing, using satellite imagery, can play an important role in these studies. In this study, changes of qualitative parameters in Gomishan wetland and Gorgan Bay located in the southeast of the Caspian Sea were investigated using Landsat images. Based on the results of the range of chlorophyll-a and water temperature changes in both ecosystems, there was an increasing trend. Evaluating of Secchi disk (transparency) ranges showed a decreasing trend until 2018. Also, among the studied years, 2009 has the lowest fluctuations. Overall, the findings of this study showed that changes in water quality parameters of Gorgan Bay and Gomishan wetland had been affected by ecological conditions and human activities such as industrial-domestic and agricultural effluents. Also, satellite data in such coastal ecosystems at different time intervals have valuable results and can be given special attention for the study of similar ecosystems.

Health risk assessment of some heavy metals detected in edible fishes of Gorgan Bay, Caspian Sea (Iran), for human.

Just recently, heavy metals have been dramatically detected in aquatic animals, especially in fishes; hence, documented method to assess their health risks for humans who eat these contaminated fishes could be helpful. For the sake of this aim, the health risk assessment of four heavy metals including arsenic, cadmium, lead, and copper in three main edible fishes caught from Gorgan bay was studied. Mullet, roach, and common carp have been caught randomly in winter and spring of 2021 from three different fishing sites of Gorgan bay, including Miankaleh wetland, estuary of Qaresu River, and mouth of Chapaghli canal. Heavy metal measurement has been done with MOOPAM method, and risk assessment of fish consumption was assessed by estimated daily (EDI) and weekly intakes (EWI), target hazard quotient (THQ), hazard index (HI), and target cancer risk (TCR) indices. In this study, estimated daily intake (EDI) was much lower than the tolerable daily intake. Although TCR of cadmium within all fishes and arsenic in two fishes were more than acceptable value, THQ and HI values were less than one, and TCR of lead as well as copper was lower than the critical values, which suggests heavy metals of the Gorgan bay have no serious risks to consumer health.

Bioaccumulation of Some Hazardous Heavy Metals in Three Edible Fishes Caught from Gorgan Bay, Caspian Sea.

A survey was conducted to evaluate heavy metal accumulation in edible fishes caught from Gorgan Bay, as a part of the Caspian Sea ecosystem. Fish samples including mullet, roach, and common carp were just randomly prepared from fishing locations in three different areas of Gorgan Bay including estuary, channel, and Miankale wetland twice the fishing seasons. A total of 135 samples were measured using an atomic absorption spectrometer to 4 heavy metals of arsenic (As), lead (Pb), cadmium (Cd), and copper (Cu) (a total of 540 metal samples). The highest accumulation of As was related to the mullet of estuary, and the lowest contamination was related to the mouth of the channel. In relation to Cd, the highest pollution in all three species related to the fishes caught from the estuary and the lowest accumulation related to the Miankaleh wetland. In the case of metal Pb, results showed the most contamination related to the Mullet caught from Miankaleh wetland and the lowest accumulation related to the roach of the mouth of the channel. In all three fish species, the highest contamination of Cu was in the estuary and the lowest was in the fish caught from Miankaleh wetland. In general, the highest accumulation belonged to all three species located in estuaries. The probable reason can be pouring river pollution to the bay more than wetlands and channel. Due to the enclosure of the Caspian Sea and consequently the Gulf of Gorgan, limiting the fishing times and points to the periods of least incoming pollutants is very helpful for public health, especially food hygiene and fishing from this ecologically sensitive and vulnerable basin.

Dietary Effects of Probiotic Lactobacillus casei on Some Immunity Indices of Common Carp (Cyprinus carpio) Exposed to Cadmium.

The present study investigated the effect of feeding common carp with Lactobacillus probiotic supplement on growth, blood indices, and immunity of serum and mucus exposed to cadmium. A total of 250 common carp with an average weight of 15 ± 0.62 were divided into three groups: fish without probiotics, and fish with two levels of probiotic. At the end of a 42-day feeding period, 50% of the lethal concentrations of cadmium were exposed for 14 days and blood and immune indices of serum and mucus were measured. The results showed that growth indices, blood indices, and serum and mucus immunity were significantly different from the control group (P < 0.05). Finally, this study showed probiotics could improve the decreasing effect of cadmium in BWI and PBWI indices and the additive effect of cadmium in FCR and ALT. Probiotic was also able to completely improve the decreasing effect of cadmium on lymphocyte indices and the additive effect of cadmium on ALP and AST and monocyte.

Assessing nitrogen dynamics model and the role of artificial lagoon in effluent loading of shrimp farms in Gomishan wetland, southern Caspian Sea.

Shrimp farming increases the nutrients, especially nitrogen in the water resources reducing water quality. This study was conducted to evaluate the nitrogen dynamics in white shrimp (Litopenaeus vannamei) farms and the role of artificial lagoon (24 ha) in reducing nitrogen levels in Gomishan coastal wetland, the eastern Caspian Sea. The results indicated that at the end of the 4-month breeding period, the amounts of nitrogen and phosphorus introduced into Gomishan wetland were calculated as to 220.157 and 39 tons, respectively in a breeding area covering 830 hectares. Nitrogen values (based on nitrate) calculated based on the relationship between the basin and the discharge of the outlet channel of the site at the time of complete emptying of the farms, were calculated to be approximately 121.8 tons per breeding time that it had an important role in eliminating about 45% of nutrient pollution and reducing the concentration of dissolved nitrogen. Moreover, nitrogen isotopic trace was observed in shrimp samples, in similar levels in the samples of both shrimp pond and lagoon, which emphasizes the role of feeding from natural food, especially benthic fauna. Overall, due to the decline of Caspian Sea water level, Gomishan coastal wetland is drying, and the output of shrimp farms is currenly the only source supplying water for the wetland. Hence, appropriate management strategies could minimize the amounts of nutrients into the natural water whilst aiding wetland's survaival.

Nanocrystalline cellulose based on chitosan hydrogel structure as a biological adsorbent for effluent of fish culture farms.

Synthetic hydrogels have been replaced by natural hydrogels due to their properties such as being long-lasting, more capacity for water absorption, high strength, and resistance. Recently, hydrogels have been defined as two- or multi-component systems consisting of three-dimensional networks of polymer chains that fill the space between macromolecules. Their water absorption efficiency depends on both polymeric nature and the capacity of the polymer network connections. Because of climate change and frequent droughts, fresh water supply has become a limiting factor for sustainable aquaculture development in Iran. Therefore, wastewater treatment and reuse can be a reasonable solution to meet the required water for expanding the aquaculture industry. The present study assessed the efficiency of a new hydrogel prepared from sugarcane waste (bagasse) to remove nitrogen and phosphate compounds from the effluent of fish farms. First, the hydrogel was prepared during the polymerization process, and then, in order to determine the optimal absorption, it was tested in a discontinuous system by performing absorption isotherm calculations. In cellulose/chitosan nanocrystalline hydrogel nanosorbent, the removal rates for nitrate, nitrite, and phosphate were 84.3%, 86%, and 90.9%, respectively. Optimal adsorption was determined at acidity of 6, time of 30 min, temperature of 40 ℃, concentration of 100 mg/L, and adsorbent dose of 0.5 g. A comparison of adsorption isotherm models showed more conformity with Freundlich and Tamkin absorption models with correlation coefficients of 0.99 and 0.97 for nitrate, 0.98 and 0.91 for nitrite, and 0.99 and 0.93 for phosphate, respectively.

Different land uses influenced on characteristics and distribution of microplastics in Qarasu Basin Rivers, Gorgan Bay, Caspian Sea.

Concerns about the negative effects of microplastics (MPs) on human health have led to increasing attention to the occurrence of MPs in the aquatic environment. Recent studies have focus on the spatio-temporal distribution of MPs in rivers for residential and agricultural areas. Qarasu River Basin, watershed to Gorgan Bay, is the site of many permanent rivers and the levels of MP pollution in those rivers are unknown. This research was conducted in three different types of land use: forest, residential, and agricultural along 8 different rivers. A total of 9 fish species were identified and the presence of MPs in 87% of fish was confirmed. The prevalence of MP among species was Liza sp. > Carassius gibelio > Gambusia holbrooki > Rutilus rutilus > Neogobius melanostomus > Cyprinus carpio = Vimba vimba > Rutilus frisii > Barbus sp. The highest frequency of MPs was observed in Qarasu River (1880 ± 251 n/kg) and Mohammadabad River (184 ± 1340 n/kg) in agricultural land use, and the lowest frequency was related to Baghu and Ziarat Rivers with 660 ± 77 and 600 ± 91 n/kg found in forest land use respectively. The highest type, color, and size of MP sediments were fragment, black, and 1-2 mm, respectively. The highest type, color, and size of MP in fishes were fiber, black, and 0.5-0.1 mm respectively. Overall according to the source tracing result, agricultural land use was the main source of MP pollution of Gorgan Bay. The Bay is strongly affected by the incoming rivers, the Qarasu River Basin as the most important river supplying water.

Dietary Beet Molasses Improved the Immune System of Common Carp (Cyprinus carpio) After Exposure to Titanium Oxide Nanoparticles, TiO2-NPs.

This study was carried out to evaluate dietary effect of beet molasses on immunity indices of common carp treated with titanium oxide nanoparticles TiO2-NPs. 250 fish were distributed in four treatments containing 0, 0.5%, 1% and 2% molasses and fed for 42 days, then fish were exposed to TiO2-NPs during a 14-day. At the end of experiment, hematological, biochemichal and mucusal immunity indices were evaluated. The aboved indices showed a significant difference compare to the control group (p < 0.05). Blood monocyte and lymphocyte in the TiO2-NPs with molasses group was reduced, that means molasses in combination with TiO2-NPs could alleviate the effect of TiO2-NPs. In treatments of TiO2-NPs and molasses combination, molasses was able to reduce the additive effect of monocyte, mean corpuscular hemoglobin concentration, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. Molasses in combination with TiO2-NPs was able to improve the negative effect of TiO2-NPs, so 2% molasses in combination with TiO2-NPs, could neutralized undesirable effect of TiO2-NPs on common carp.

Dietary Effect of Probiotic and Prebiotic on Some Mucus Indices of Silver Carp (Hypophthalmichthys molitrix) Exposed to Silver Nanoparticles.

Prebiotic and probiotic supplements can directly activate fish primary defense mechanisms by acting on responsible receptors. This study aimed to investigate the effect of using Pediococcus acidilactici and Agaricus bisporus separately and in combination with silver carp's mucosal immune system indices and finally the possibility of reducing the toxicity of silver nanoparticles in silver carp. After calculating LC50, the sub-lethal toxicity test was performed to sub-lethal concentration of silver nanoparticles for 14 days. Fish skin mucus was collected in polyethylene bags containing 10 ml of 50 mM sodium chloride for future analysis. Results of skin mucosal indices activity showed significant changes in treatments fed with additives compared to the control treatment after exposure to silver nanoparticles on days 1, 7, and 14 in including lysozyme enzyme activity, total protein, and skin mucosal protein pattern. The addition of additives will improve the relative mucosal safety indices of silver carp. The results of statistical analysis showed that the addition of combination supplements, Pediococous acidilactici, and Agaricus bisporus powder could affect the skin mucosal safety indices. It is effective and ultimately improves the immune system function of silver carp skin in 14-day exposure to silver nanoparticles.

Biomagnification of Copper Along the Aquatic Food Chain (Artemia franciscana, Danio rerio, and Astronotus ocellatus).

Nowadays, pollution of aquatic ecosystems with heavy metals is one of the most important environmental challenges due to toxicity, stability, and bioaccumulation in the environment. Heavy metals accumulate in aquatics and plant tissues and can eventually threaten human health by transmission into the food chain. For this reason, in the present study, the effect of copper sulfate (CuSO4) and its transmission in the food chain of Artemia franciscana, Danio rerio (Zebra), and Astronotus ocellatus (Oscar) were investigated. Initially, lethal concentration of Cu on Artemia was obtained in 24 h. In the first step, Zebra fishes fed with Artemia (under copper sulfate exposure (LC50)) and water (clean and 10% of CuSO4) treatments. In the second step, Oscar fishes fed with Zebra (under copper sulfate exposure (LC50)) and water (clean and 10% of CuSO4) treatments. In the last step, the concentration of Cu was measured in liver tissues of Zebra and Oscar fishes. Also, the variation of glucose and enzymes including Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), and Autologous Conditioned Plasma (ACP) was measured. The results of this study showed that Cu causes histopathological changes liver tissues including hyperemia, necrosis, hepatic portal vein, hepatocyte destruction, nucleolysis, bile stasis, and biliary edema. Also, a strong positive and significant correlation was observed between Cu and increasing glucose and ALT, AST, and ACP.

Size-dependent effects of microplastic on uptake, immune system, related gene expression and histopathology of goldfish (Carassius auratus).

Todays, with the industrialization of human societies, pollution of aquatic ecosystems with plastics derivatives are a serious concern, affecting the life of their organisms. The present study was conducted to investigate the size effects of micro-plastic, polystyrene on some physiological lesions of the goldfish, Carassius auratus. Fish were exposed to two sizes (0.25 and 8 µm) polystyrene at different environmentally relevant concentrations. The exposure trial was done in two steps. First, fish exposed to a stable concentration of 300 mg/L polystyrene for 168 h. Gill, intestine, and liver tissues were sampled every 24 h to investigate the accumulation of polystyrene. Then, fish were exposed in three replicates to 0 (control), 0.05, 0.5, and 5 mg/L polystyrene in two sizes of 0.25 and 8 µm for 28 days. After the exposure period, gill, liver, and intestine tissues were sampled for histological study, also, serum samples were collected for biochemical assays. Fluorescent microscope observations confirmed the accumulation of polystyrene in tissue samples with time. In addition, histological lesions were found in the liver, intestine, and gill of the exposed fish. The severity of lesions showed a size and dose-dependent pattern. Polystyrene induced the antioxidant system of exposed fish through elevating the levels of SOD and CAT activity and significant difference in expression of antioxidant related genes (CAT, SOD and HSP70). In conclusion, the results of the present study confirmed the toxic effects of microplastic, polystyrene on goldfish.

Effects of polystyrene microparticles on inflammation, antioxidant enzyme activities, and related gene expression in Nile tilapia (Oreochromis niloticus).

Due to the importance of Nile tilapia in the aquaculture sector, the present study aimed to evaluate the adverse impacts of the polystyrene microparticles (PS-MPs) on inflammation, immune, and antioxidative responses. For this reason, fish were exposed to waterborne PS-MPs at two sizes (9 µm and 0.35 µm) beside the control group for 28 days. Regardless of particle size, the final weight, weight gain, specific growth rate, and feed conversion ratio of Nile tilapia were not influenced by the PS-MPs exposure (P > 0.05). Superoxide dismutase (SOD), glutathione peroxidase (GPX), and malondialdehyde (MDA) levels were significantly increased in the group exposed to 9 µm and followed by those exposed to 0.35 µm while the lowest SOD, GPX, and MDA were in the control group. The expression of catalase (CAT) and lysozyme genes were upregulated in the group of fish exposed to 9 µm (P < 0.05). The expression of the interferon-gamma (IFN-γ) gene was upregulated in fish exposed to 9 µm and 0.35 µm when compared with the control (P < 0.05). The expression of interleukin 8 (IL8), interleukin (IL-1ß), and tumor necrosis factor (TNF-α) genes was upregulated in the group of fish exposed to 9 µm (P < 0.05). The hepatic mRNA levels of cytochrome P450 (CYP450) gene were significantly upregulated in the group of fish exposed to 9 µm when compared with the control (P < 0.05) without significant differences with those exposed to 0.35 µm (P > 0.05). The obtained results refer to the adverse effects of PS-MPs on the antioxidative, inflammatory, and detoxification-related genes of Nile tilapia.