Foliar residue dynamics of azadirachtins following direct stem injection into white and green ash trees for control of emerald ash borer.

BACKGROUND: Azadirachtins are natural insecticides derived from the neem tree. The emerald ash borer (EAB) is an exotic invasive insect pest that infests various ash tree species and has the potential for significant economic, aesthetic and ecological impacts throughout North America. The initial translocation and foliar residue dynamics of azadirachtins were examined following direct injection into white and green ash trees growing in urban scenarios as a potential control for EAB. RESULTS: Substantial concentrations of azadirachtins A and B [mean maxima > 0.98 mg kg(-1) fresh weight (f.w.)] were observed within 2 days of injecting a specifically designed formulation of azadirachtins. Foliar residues declined exponentially through time, with half-life estimates ranging from 5.1 to 12.3 days. At the time of leaf senescence, foliar residue levels approximated 0.01 mg kg(-1) f.w., strongly mitigating the potential effects of non-target biota in soil or aquatic compartments. CONCLUSION: The magnitude and duration of exposures observed in this field study were considered to be above the thresholds required for biological effectiveness against both larval and adult life stages of EAB. Results support the use of azadirachtins as an environmentally acceptable systemic insecticide for control of EAB and protection of high-value ash trees in urban environments.

Azadirachtin: an effective systemic insecticide for control of Agrilus planipennis (Coleoptera: Buprestidae).

The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive pest discovered in North America in 2002, is now well established and threatens ash (Fraxinus spp.) trees throughout the continent. Experiments were conducted to 1) examine the efficacy of an alternative natural pesticide, azadirachtin, to control emerald ash borer, and 2) determine foliar uptake and dissipation patterns after systemic injections of azadirachtin into trunks of small (2.2 cm diameter at breast height [dbh]), uninfested green ash trees. We found no evidence of mortality of adult beetles. In contrast, fewer larvae completed their development at dose levels > or = 1.7 mg (AI)/cm dbh and development ceased beyond the second instar at dose levels > or = 13.6 mg (AI)/cm dbh. Substantial concentrations (11.2 microg/g dry mass [SD = 7.55]) of azadirachtin were present in leaves within 7 d of treatment. After rapid initial uptake, concentrations in leaves declined logarithmically during the 55 d after injection. A similar pattern was observed in a separate experiment that examined the uptake and translocation of azadirachtin in larger green ash trees (22 cm dbh) treated with 250 mg (AI) /cm dbh with the EcoJect injection system. In another experiment, recently infested plantation green ash trees treated with doses > or = 40 mg (AI)/cm dbh had significant reductions in adult emergence approximately 1 yr postinjection. Given the inhibition of larval development, reduction of adult emergence, and the occurrence of foliar residues at biologically active concentrations, we conclude that azadirachtin is effective in protecting ash trees from emerald ash borer.

Laboratory evaluation of two novel strategies to control first-instar gypsy moth larvae with spinosad applied to tree trunks.

Two strategies for controlling first-instar larvae of gypsy moth (Lymantria dispar (L)), insecticidal bait and contact insecticide applied directly to the tree trunk, were evaluated in the laboratory. Spinosad was selected as a candidate natural-product insecticide that is active both by contact and ingestion. Incorporated into artificial diet-based bait, spinosad was toxic to neonate larvae with a minimal 10-s feeding period, with an LC50 value of 20 (15-26, 95% confidence interval) mg liter-1. It was significant that neonate larvae did not discriminate between spinosad-treated and control diet. Efficacy of diet-based bait in the laboratory, however, was significantly impacted by previous exposure to diet; fed larvae did not stop at the bait and did not incur mortality, as compared to unfed larvae. Oak bark was a suitable substrate from which neonate larvae could contact spinosad residues. Spinosad applied directly to oak bark resulted in significant mortality after 1- and 4-min crawling contact exposure times (LC50 = 24 [20-29, 95% CI] and 8.7 [6.9-11, 95% CI] mg liter-1, respectively) and contact activity persisted for 2 weeks. While contact activity was more potent on glass surfaces than on oak bark, the LC50 values differed only by factors of 2.4 and 3.6, for 1- and 4-min exposures respectively.