Predicted Three-Dimensional Structure of the GCR1 Putative GPCR in Arabidopsis thaliana and Its Binding to Abscisic Acid and Gibberellin A1.

GCR1 has been proposed as a plant analogue to animal G-protein-coupled receptors that can promote or regulate several physiological processes by binding different phytohormones. For instance, abscisic acid (ABA) and gibberellin A1 (GA1) have been shown to promote or regulate germination and flowering, root elongation, dormancy, and biotic and abiotic stresses, among others. They may act through binding to GCR1, which would put GCR1 at the heart of key signaling processes of agronomic importance. Unfortunately, this GPCR function has yet to be fully validated due to the lack of an X-ray or cryo-EM 3D atomistic structure for GCR1. Here, we used the primary sequence data from Arabidopsis thaliana and the GEnSeMBLE complete sampling method to examine 13 trillion possible packings of the 7 transmembrane helical domains corresponding to GCR1 to downselect an ensemble of 25 configurations likely to be accessible to the binding of ABA or GA1. We then predicted the best binding sites and energies for both phytohormones to the best GCR1 configurations. To provide the basis for the experimental validation of our predicted ligand-GCR1 structures, we identify several mutations that should improve or weaken the interactions. Such validations could help establish the physiological role of GCR1 in plants.

Combined Manufacturing Process of Copper Electrodes for Micro Texturing Applications (AMSME).

Surface texturing has brought significant improvements in the functional properties of parts and components. Sinker electro discharge machining (SEDM) is one of the processes which generates great texturing results at different scale. An electrode is needed to reproduce the geometry to be textured. Some geometries are difficult or impossible to achieve on an electrode using conventional and even unconventional machining methods. This work sets out the advances made in the manufacturing of copper electrodes for electro erosion by additive manufacturing, and their subsequent application to the functional texturing of Al-Cu UNS A92024-T3 alloy. A combined procedure of digital light processing (DLP) additive manufacturing, sputtering and micro-electroforming (AMSME), has been used to produce electrodes. Also, a specific laboratory equipment has been developed to reproduce details on a microscopic scale. Shells with outgoing spherical geometries pattern have been manufactured. AMSME process has shown ability to copper electrodes manufacturing. A highly detailed surface on a micrometric scale have been achieved. Copper shells with minimum thickness close to 300 µm have been tested in sinker electro discharge machining (SEDM) and have been shown very good performance in surface finishing operations. The method has shown great potential for use in surfaces texturing.

African American College Students' Drinking Behaviors and Their Relationship to Self-Efficacy and Positive or Negative Expectancies Regarding Alcohol Consumption.

College students' alcohol consumption remains a significant concern for colleges and universities. However, most research overwhelmingly utilizes White samples from predominantly White universities, limiting knowledge of African American students' drinking behaviors on historically Black campuses. This study examined alcohol usage among African American college students by investigating relationships between alcohol consumption and positive and negative expectancies as well as self-efficacy. A convenience sample of 282 students was used. The Alcohol Use Disorders Identification Test (AUDIT) measured alcohol consumption and identified individuals whose consumption created hazardous drinking patterns. Alcohol expectancy was measured by the Alcohol Effects Questionnaire (AEQ), and the Spheres of Control Scale measured self-efficacy. Students in this sample tended to believe that alcohol consumption was linked with more negative than positive alcohol expectancy beliefs. Alcohol expectancies, specifically positive expectancies, appeared to play a significant role in predicting alcohol consumption. There was also a positive relationship between positive expectancies and alcohol consumption. Despite these results, our regression model was only able to account for about 20% of the variance (r2 = 0.187). These findings are important in developing prevention and intervention programs to address the pervasive and critical social ills and reduce alcohol consumption among African American college students.

Review of Online Education in Social Work Programs.

Despite the growing prevalence of online courses and programs, there is a lack of information on and continuing debates about their effectiveness on student learning outcomes in social work education.Purpose: The current study was to examine the status of the evaluation of online courses or programs in social work education.Method: Using comprehensive and rigorous methods, 15 articles were reviewed to examine the effectiveness of social work online courses or programs. All reviewed articles were either quantitative or qualitative studies that used process or outcome evaluation.Results: The major criterion for comparison of the effectiveness between the face-to-face and online course was grades.Discussion: There is an overall disconnect with the concrete value and meaning of assessment within social work programs and universities and schools appear to have placed assessment and student learning outcomes secondary to the financial gains that online courses and programs bring.

Termination of parental rights for parents with substance use disorder: for whom and then what?

The purpose of this study is to investigate the correlates of termination of parental rights (TPR) for parents with substance use disorder (SUD) and to determine what happens with regard to permanency once a TPR decision is made. Bivariate techniques and hierarchical nonlinear modeling are used. Parents of older youth, boys, and Hispanics were less likely, whereas parents who failed to make progress in substance use treatment and parenting skills are more likely to experience TPR. At follow up, 85% of the children were adopted, 7% remained in a substitute care settings, and 7% were reunified with their parents. Concerns remain for children without a permanent home setting.

Brokering language and culture: can ad hoc interpreters fill the language service gap at community health centers?

The purpose of the research was to explore the ability of ad hoc interpreters to integrate into the organizational climate at a federally qualified community health clinic (CHC) and create satisfactory services for limited-English-proficiency clients. Survey and interview data were gathered from staff (n = 17) and Latino clients (n = 30). The data indicate that clients felt satisfied with interpreters. Some friction existed between the interpreters and the medical staff due to incongruent expectations. The CHC's organizational climate and the interpreters' commitment to the Latino community mediated the impact of these tensions on services and satisfaction. The study offers important insight into how ad hoc interpreters can become professional medical interpreters within a limited-resource service environment.