Asunto(s)
Cuidados Preoperatorios , Disrafia Espinal/diagnóstico , Adulto , Anestesia Epidural , Anestesia Raquidea , Contraindicaciones de los Procedimientos , Fístula Cutánea/cirugía , Diagnóstico Tardío , Femenino , Humanos , Hallazgos Incidentales , Lipoma/etiología , Región Lumbosacra , Imagen por Resonancia Magnética , Examen Físico , Neoplasias de la Médula Espinal/etiologíaRESUMEN
INTRODUCTION: Since Pall-German stopped manufacturing ITLC-SG, it has become necessary to validate alternative stationary phases. OBJECTIVE: To validate different stationary phases versus ITLC-SG Pall-Gelman in the determination of the radiochemical purity (RCP) of (111)In-pentetreotide ((111)In-Octreoscan) by planar chromatography. MATERIAL AND METHODS: We conducted a case-control study, which included 66 (111)In-pentetreotide preparations. We determined the RCP by planar chromatography, using a freshly prepared solution of 0,1M sodium citrate (pH 5) and the following stationary phases: ITLC-SG (Pall-Gelman) (reference method), iTLC-SG (Varian), HPTLC silica gel 60 (Merck), Whatman 1, Whatman 3MM and Whatman 17. For each of the methods, we calculated: PRQ, relative front values (RF) of the radiopharmaceutical and free (111)In, chromatographic development time, resolution between peaks. We compared the results obtained with the reference method. The statistical analysis was performed using the SPSS program. The p value was calculated for the study of statistical significance. RESULTS: The highest resolution is obtained with HPTLC silica gel 60 (Merck). However, the chromatographic development time is too long (mean=33.62minutes). Greater resolution is obtained with iTLC-SG (Varian) than with the reference method, with lower chromatographic development time (mean=3.61minutes). Very low resolutions are obtained with Whatman paper, essentially with Whatman 1 and 3MM. Therefore, we do not recommend their use. CONCLUSIONS: Although iTLC-SG (Varian) and HPTLC silica gel 60 (Merck) are suitable alternatives to ITLC-SG (Pall-Gelman) in determining the RCP of (111)In-pentetreotide, iTLC-SG (Varian) is the method of choice due to its lower chromatographic development time.
Asunto(s)
Cromatografía en Papel/métodos , Cromatografía en Capa Delgada/métodos , Radioisótopos de Indio/análisis , Radiofármacos/análisis , Somatostatina/análogos & derivados , Cromatografía en Papel/instrumentación , Cromatografía en Capa Delgada/instrumentación , Contaminación de Medicamentos , Papel , Valores de Referencia , Gel de Sílice , Somatostatina/análisisAsunto(s)
Adenocarcinoma/secundario , Neoplasias Primarias Múltiples/diagnóstico por imagen , Neurilemoma/diagnóstico por imagen , Neoplasias del Sistema Nervioso Periférico/diagnóstico por imagen , Neoplasias de la Próstata/diagnóstico por imagen , Adenocarcinoma/diagnóstico por imagen , Anciano , Reacciones Falso Positivas , Ingle , Humanos , Metástasis Linfática , Masculino , CintigrafíaAsunto(s)
Aneurisma de la Aorta Abdominal/diagnóstico por imagen , Aneurisma de la Aorta Torácica/diagnóstico por imagen , Hallazgos Incidentales , Imagen de Perfusión , Trombosis/diagnóstico por imagen , Aortografía , Dolor en el Pecho/etiología , Diagnóstico Diferencial , Disnea/etiología , Humanos , Masculino , Persona de Mediana Edad , Embolia Pulmonar/diagnóstico , Trombosis/complicaciones , Tomografía Computarizada por Rayos XAsunto(s)
3-Yodobencilguanidina , Neoplasias de la Médula Ósea/diagnóstico por imagen , Neoplasias de la Médula Ósea/secundario , Radioisótopos de Yodo , Neuroblastoma/diagnóstico por imagen , Neuroblastoma/secundario , Radiofármacos , Neoplasias de las Glándulas Suprarrenales/diagnóstico por imagen , Preescolar , Humanos , Ilion/diagnóstico por imagen , Masculino , Cintigrafía , Medronato de Tecnecio Tc 99m/análogos & derivados , Tibia/diagnóstico por imagenAsunto(s)
Defectos de los Tabiques Cardíacos/diagnóstico por imagen , Hipertensión Pulmonar/diagnóstico por imagen , Pulmón/diagnóstico por imagen , Embolia Pulmonar , Adulto , Encéfalo/diagnóstico por imagen , Diagnóstico Diferencial , Femenino , Fibrosis , Defectos de los Tabiques Cardíacos/complicaciones , Enfermedad de Hodgkin/complicaciones , Enfermedad de Hodgkin/radioterapia , Humanos , Hipertensión Pulmonar/etiología , Hallazgos Incidentales , Riñón/diagnóstico por imagen , Mediastino/lesiones , Mediastino/patología , Mediastino/efectos de la radiación , Ventilación Pulmonar , Traumatismos por Radiación/etiología , Traumatismos por Radiación/patología , Cintigrafía , Radiofármacos/farmacocinética , Agregado de Albúmina Marcado con Tecnecio Tc 99m/farmacocinética , Distribución Tisular , Tomografía Computarizada por Rayos XRESUMEN
OBJECTIVE: To analyse the behaviour of serum thyroglobulin (Tg), antithyroglobulin antibodies (TgAb), thyrotropin (TSH), free thyroxine (FT4) and total triiodothyronine (TT3) levels at each time during the rhTSH stimulation protocol in patients with differentiated thyroid carcinoma (DTC). MATERIALS AND METHODS: We carried out 117 rhTSH stimulations in DTC patients. We determined the serum levels of Tg, TgAb, TSH, FT4 and TT3 at baseline and 24, 48 and 96 hours after beginning stimulation, using RIA or IRMA. The software program SPSS 15.0 was used for statistical analysis of data. RESULTS: We found statistically significant differences between the mean Tg values at different times (2.08 ng/ml baseline; 2.64 ng/ml at 24 hours; 4.98 ng/ml at 48 hours; 6.59 ng/ml at 96 hours), reaching maximum values at 96 hours. During this time, we observed the highest percentage of pathological values. After administration of rhTSH, there was a significant increase in the mean TSH value (98.88 mIU/l at 24 hours; 111.10 mIU/l at 48 hours). The mean TSH value at 96 hours decreased approximately 5 times with respect to the mean 48 hour value. We did not observe changes in the TgAb, FT4 or TT3 levels. CONCLUSIONS: The assessment of Tg after rhTSH stimulation should be performed 96 hours after beginning stimulation. Administration of rhTSH causes a significant elevation in serum TSH levels, without modifying serum TgAb, FT4 or TT3 levels.