RESUMEN
Southeast Asia is particularly susceptible to the negative impacts of increasing coastal pollution as coastal populations and cities grow at unprecedented rates. Although water chemistry can be monitored, there are greater advantages in using bioindicators as reflectors of the combined effect of multiple pollution types on coastal ecosystem health and for early detection of the negative impacts of pollutants on biotic systems. This study explores the utility and application of ostracods as pollution bioindicators and examines the response of ostracod assemblages to variable pollution in Lap An Lagoon, central Vietnam. From 14 sites within the lagoon, 79 species of 46 genera were identified and sediment grain size, total organic carbon, organic matter and heavy metal concentration were measured. Cluster analysis, detrended correspondence analysis and canonical correspondence analysis identified four distinct ostracod biofacies that were highly correlated to the physical environmental variables (salinity, depth, sediment type, heavy metal concentrations, total organic carbon and organic matter) and are shown to be the main factors controlling ostracod biofacies. Low ostracod diversities were found in silty sediments with heavy metal concentrations likely toxic. Sinocytheridea impressa was indicative of a marginally polluted environment within the lagoon. This study provides evidence for the potential for Southeast Asian ostracods to be used in water quality assessments and the data collected can be used as a baseline for future pollution monitoring.
Asunto(s)
Metales Pesados , Contaminantes Químicos del Agua , Animales , Crustáceos , Ecosistema , Monitoreo del Ambiente , Sedimentos Geológicos , Metales Pesados/análisis , Vietnam , Contaminantes Químicos del Agua/análisisAsunto(s)
Proteínas de Unión a Calmodulina/aislamiento & purificación , Calmodulina/aislamiento & purificación , Proteínas Recombinantes de Fusión/aislamiento & purificación , Secuencia de Aminoácidos , Animales , Fusión Artificial Génica/métodos , Secuencia de Bases , Calmodulina/química , Calmodulina/genética , Proteínas de Unión a Calmodulina/química , Proteínas de Unión a Calmodulina/genética , Cromatografía de Afinidad/métodos , Clonación Molecular/métodos , Electroforesis en Gel de Poliacrilamida/métodos , Epítopos , Escherichia coli/genética , Escherichia coli/crecimiento & desarrollo , Regulación Bacteriana de la Expresión Génica/efectos de los fármacos , Vectores Genéticos , Humanos , Isopropil Tiogalactósido/farmacología , Datos de Secuencia Molecular , Oligopéptidos , Péptidos , Plásmidos , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genéticaRESUMEN
Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter.