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1.
Mikrochim Acta ; 191(7): 434, 2024 06 29.
Artículo en Inglés | MEDLINE | ID: mdl-38951317

RESUMEN

An enhanced lateral flow assay (LFA) is presented for rapid and highly sensitive detection of acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antigens with gold nanoflowers (Au NFs) as signaling markers and gold enhancement to amplify the signal intensities. First, the effect of the morphology of gold nanomaterials on the sensitivity of LFA detection was investigated. The results showed that Au NFs prepared by the seed growth method showed a 5-fold higher detection sensitivity than gold nanoparticles (Au NPs) of the same particle size, which may benefit from the higher extinction coefficient and larger specific surface area of Au NFs. Under the optimized experimental conditions, the Au NFs-based LFA exhibited a detection limit (LOD) of 25 pg mL-1 for N protein using 135 nm Au NFs as the signaling probes. The signal was further amplified by using a gold enhancement strategy, and the LOD for the detection of N protein achieved was 5 pg mL-1. The established LFA also exhibited good repeatability and stability and showed applicability in the diagnosis of SARS-CoV-2 infection.


Asunto(s)
Antígenos Virales , Proteínas de la Nucleocápside de Coronavirus , Oro , Límite de Detección , Nanopartículas del Metal , SARS-CoV-2 , Oro/química , SARS-CoV-2/inmunología , Nanopartículas del Metal/química , Humanos , Antígenos Virales/análisis , Antígenos Virales/inmunología , Proteínas de la Nucleocápside de Coronavirus/inmunología , Proteínas de la Nucleocápside de Coronavirus/análisis , Fosfoproteínas/inmunología , Fosfoproteínas/análisis , Fosfoproteínas/química , COVID-19/diagnóstico , COVID-19/virología , Inmunoensayo/métodos , Prueba Serológica para COVID-19/métodos
2.
Talanta ; 275: 126156, 2024 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-38692048

RESUMEN

The development of simple methods for the isolation and quantification of exosomes in biological samples is important. By using the typical two-dimensional (2D) nanomaterials, graphene oxide (GO), the present work first studied the interaction of liposomes with the nanocomposites formed by adsorbing HRP on the GO surface and found the presence of liposomes led to the release of HRP from the GO surface to the solution phase triggering the luminol-H2O2 chemiluminescence (CL) reaction to emit light. Benefiting from the similarity of exosomes to liposomes in both composition and morphology aspects, the GO-HRP nanocomposites with a mass ratio of 120:1 and 160:1 were employed for the quantitative detection of exosomes in 100-fold diluted serum samples. The whole detection process took about 15 min and as low as 3.2 × 102 particles µL-1 of exosomes could be sensitively detected. In addition to GO-HRP nanocomposites, the CL responses of other nanocomposites obtained from adsorbing HRP on other 2D nanomaterials such as layered MoS2 for exosomes were also tested. MoS2-HRP exhibited similar behavior and the LODs for the detection of exosomes were 5.8 × 102 particles µL-1. The proposed assays were a biomarker-independent quantitative method that achieved the quantification of exosomes in serum samples directly without an isolation process.


Asunto(s)
Exosomas , Grafito , Peroxidasa de Rábano Silvestre , Mediciones Luminiscentes , Nanoestructuras , Exosomas/química , Grafito/química , Peroxidasa de Rábano Silvestre/química , Mediciones Luminiscentes/métodos , Adsorción , Humanos , Nanoestructuras/química , Luminol/química , Molibdeno/química , Disulfuros/química , Peróxido de Hidrógeno/química , Límite de Detección , Liposomas/química , Nanocompuestos/química
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