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1.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-1030453

RESUMEN

Objective To analyze the heat-clearing and anti-inflammatory mechanism of Xiaoyan Tuire Granules by network pharmacology,and to determine the 8 main active components of Xiaoyan Tuire Granules by ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS/MS).Methods SwissTargetPrediction database was used to screen the target of components absorbed in the blood of Xiaoyan Tuire Granules.The main target of disease was obtained by CTD database,and the protein-protein interaction(PPI)was analyzed by String platform.GO and KEGG enrichment analysis were conducted using the DAVID database,followed by the construction of the components-targets-pathways network using Cytoscape software.Finally,the molecular docking verification of the key compound-target was conducted.The main active components in Xiaoyan Tuire Granules,including indirubin,isoliquiritigenin,liquiritigenin,glycyrrhizinic acid,liquiritin,esculetin,caffeic acid and chlorogenic acid,were determined by UPLC-MS/MS method.Analysis was performed on a WATERS ACQUITY UPLC? BEH C18 column(2.1 mm×100 mm,1.7 μm)with gradient elution of 0.1%formate in acetonitrile(A)-0.1%formate-5 mmol·L-1 ammonium formate(B),and the determination was carried out in multiple reaction monitoring(MRM)mode.Results Sixteen potential active components,such as indirubin,esculetin,and caffeic acid were identified by network pharmacology and molecular docking,and 10 core targets including transcription factor AP-1(JUN),adhesive connexin β1(CTNNB1)and cysteine aspartic protease-3(CASP3)were predicted.The pathway enrichment analysis revealed that heat-clearing and anti-inflammatory effects of Xiaoyan Tuire Granules were mainly related to signaling pathways such as interleukin-17(IL-17)and hypoxia-induced cause-1(HIF-1).Molecular docking experiments showed that its main active components docked well with core targets.The results of content determination exhibited that all components had good linear relationship within certain concentration range(r>0.999),good precision,repeatability,and stability.The contents of 8 components in 16 batches of samples were 0.94-3.41,0.99-5.61,0.80-5.84,85.48-141.11,4.30-10.09,152.35-271.80,11.31-26.94,1.99-5.58 μg·g-1,respectively.The contents of indirubin,isoliquiritigenin,liquiritigenin,liquiritin,and chlorogenic acid in Xiaoyan Tuire Granules from two manufacturers were significantly different.Conclusion This study preliminarily revealed the mechanism of Xiaoyan Tuire Granules,which exerted heat-clearing and anti-inflammatory effects through multiple components,targets and pathways.A method for the determination of multiple components in Xiaoyan Tuire Granules was established.This study may provide a reference for its pharmacological research and quality control.

2.
Rev Neurosci ; 31(2): 143-159, 2020 01 28.
Artículo en Inglés | MEDLINE | ID: mdl-31539363

RESUMEN

The discovery of neural stem cells (NSCs) and their microenvironment, the NSC niche, brought new therapeutic strategies through neurogenesis and angiogenesis for stroke and most neurodegenerative diseases, including Alzheimer's disease. Based on the close links between NSCs and endothelial cells, the integration of neurogenesis and angiogenesis of the NSC niche is also a promising area to the neurovascular unit (NVU) modeling and is now offering a powerful tool to advance our understanding of the brain. In this review, critical aspects of the NVU and model systems are discussed. First, we briefly describe the interaction of each part in the NSC niche. Second, we introduce the co-culture system, microfluidic platforms, and stem cell-derived 3D reconstitution used in NVU modeling based on the close relations between NSCs and endothelial cells, and various characteristics of cell interactions in these systems are also described. Finally, we address the challenges in modeling the NVU that can potentially be overcome by employing strategies for advanced biomaterials and stem cell co-culture use. Based on these approaches, researchers will continue to develop predictable technologies to control the fate of stem cells, achieve accurate screening of drugs for the nervous system, and advance the clinical application of NVU models.


Asunto(s)
Encéfalo/citología , Comunicación Celular/fisiología , Células Endoteliales/patología , Células-Madre Neurales/citología , Neurogénesis/fisiología , Enfermedad de Alzheimer/patología , Animales , Encéfalo/irrigación sanguínea , Humanos
3.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-484335

RESUMEN

Aim To figure out whether Lonicera mac-ranthoides could induce hemolysis. Methods In vitro, macroscopic observation and spectrophotometry were used to observe whether the solutions of extracts from Lonicera macranthoides, MacranthoidinB and Dipsa-cosideB could induce hemolysis in 2% red cell suspen-sion of New Zealand white rabbits. And the three test-ed materials were prepared in concentration gradient of 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 mg· L - 1; and in vivo, mice were respectively treated with MacranthoidinB (0. 110 g·kg - 1 , 0. 055 g·kg - 1 ), DipsacosideB(0. 020 g·kg - 1 , 0. 010 g·kg - 1 ), ex-tracts (2. 275 g·kg - 1 , 1. 137 g·kg - 1 , crude drugs) once per day for 7 days, and all of the tested doses de-pended on the clinical doses. Then, RBC, RET and MCHC before and after administration were tested. Re-sults The hemolytic ratio in each treated group was below 5% in vitro. And in vivo, the three materials did not induce hemolysis and had no significant influence on RBC,RET and MCHC(P > 0. 05). Conclusions Extracts from flower bud of Lonicera macranthoides, MacranthoidinB and DipsacosideB, have not caused hemolysis in vivo and in vitro in this research.

4.
Chinese Journal of Burns ; (6): 37-41, 2015.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-311913

RESUMEN

<p><b>OBJECTIVE</b>To explore the influence of hydrogen sulfide on the intestinal biological barrier, by applying exogenous hydrogen sulfide and hydrogen sulfide synthase inhibitor for the treatment of rats with severe burn injury.</p><p><b>METHODS</b>One hundred and four SD rats were divided into sham injury (SI, n = 8), burn control (BC, n = 32), sodium hydrosulfide (SH, n = 32), and propargylglycine groups (PPG, n = 32) according to the random number table. Rats in group SI were sham injured without fluid resuscitation. Rats in the latter 3 groups were inflicted with 30% TBSA full-thickness scald (referred to as burn below) on the back and intraperitoneally injected with 40 mL/kg balanced salt solution immediately after injury. Rats in groups SH and PPG were respectively intraperitoneally injected with SH (56 µmol/kg) and PPG (45 mg/kg) within 1 hour post injury. From post injury day (PID) 2, SH (56 µmol/kg) and PPG (45 mg/kg) were respectively intraperitoneally injected once a day to rats in groups SH and PPG. Eight rats from groups BC, SH, and PPG were sacrificed on PID 2, 7, 14 and 21, and ceca samples were collected. Ceca samples were added to the appropriate culture medium after being homogenized and diluted, for the culture of Bifidobacterium, Lactobacillus, Enterococcus, Enterobacter, and Candida albicans. The content of bacteria was calculated after the bacteria number was counted. The same procedure was performed for rats in group SI. Data were processed with logarithmic function, one-way analysis of variance, analysis of variance of factorial design, and SNK-q test.</p><p><b>RESULTS</b>On each PID, the content of Bifidobacterium and Lactobacillus in the ceca of each group with burned rats was less than that of group SI (with q values from 4.12 to 20.74, P values below 0.05); while the content of Enterococcus, Enterobacter, and Candida albicans was more than that of group SI (with q values from 2.84 to 68.29, P values below 0.05). Compared with that of group BC, the content of Bifidobacterium and Lactobacillus in the ceca of rats in group SH were increased on each PID (with q values from 2.88 to 17.57, P values below 0.05). In group SH, the content of Bifidobacterium peaked as (6.54 ± 0.35) lg (CFU/g) on PID 7, the content of Lactobacillus peaked as (7.25 ± 0.71) lg (CFU/g) on PID 21. Compared with that of group BC, the content of Enterococcus, Enterobacter, and Candida albicans in the ceca of rats in group SH was reduced on each PID (with q values from 2.79 to 29.59, P values below 0.05). Compared with that of group BC, the content of Bifidobacterium and Lactobacillus in the ceca of rats in group PPG was decreased on each PID (with q values from 2.82 to 46.56, P values below 0.05); while the content of Enterococcus, Enterobacter, and Candida albicans was significantly increased on each PID (with q values from 2.93 to 41.42, P values below 0.05). In group PPG, the content of Enterococcus peaked as (9.41 ± 0.22) lg (CFU/g) on PID 21, the content of Enterobacter peaked as (9.96 ± 0.24) lg (CFU/g) on PID 14, and that of Candida albicans peaked as (3.94 ± 0.84) lg (CFU/g) on PID 14.</p><p><b>CONCLUSIONS</b>Exogenous hydrogen sulfide can subdue the growth of pathogenic bacteria while promote that of probiotics, thus helping maintain the integrity of intestinal biological barrier of rats with burn injury.</p>


Asunto(s)
Animales , Ratas , Quemaduras , Quimioterapia , Patología , Sulfuro de Hidrógeno , Farmacología , Usos Terapéuticos , Intestinos , Patología , Ratas Sprague-Dawley , Traumatismos de los Tejidos Blandos
5.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-592317

RESUMEN

Objective To provide information collection,processing and inquiry system,which is safe,accurate rapid and in real time,for combat readiness medical supplies.Methods RFID technology and series antenna coils separated in space were used in combat readiness medicine-chest,thus enabling the medicine-chest to have the virtue of intelligence.Results The combat readiness medicine-chest equipped with RFID system can automatically classify to count and manage medical supplies in medicine-chest without opening the chest.The medical supplies can be monitored in real time and the information of outdated medicine can be automatically prompted.Conclusion Intelligent medicine-chest based on RFID technology can provide informatized method and technique for the management of combat readiness medical supplies for PLA.

6.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-592565

RESUMEN

Objective To identify the location of medicines in combat readiness medicine cabinet.Methods RFID system was used in combination with series antennas separated in space in combat readiness medicine cabinet.Results The combat readiness medicine cabinet equipped with RFID system could identify the location of medicines in medicine cabinet.It realized the location management and automatic classification statistics for medicines without opening cabinet.It greatly shortened the time in taking medicine in field emergency care.Conclusion Intelligent medicine cabinet based on RFID technology can upgrade the modernization level of sanitation logistics and provide a modern management method for iatrical material of combat readiness of PLA.

7.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-593319

RESUMEN

Objective In order to increase the operation coverage of high-frequency (HF) RFID reader without increasing the power output, a novel RFID antenna for multi-drawer intelligent medicine-chest are proposed. Using this antenna, the RFID tags on medicine can be read effectively. Methods Several small antenna coils can be combined in series or parallel connection to make a more efficient RFID reader antenna. The use of small coil will be helpful to eliminate the blind spot of RFID reader with large coil antenna. Results The medicine-chest's size is 58 cm?50 cm?62 cm3, which includes two or three layers. We design four combined small antenna coils to cover the drawer. The test result shows that the antenna read region is about 54 cm?48 cm?30 cm, all RFID tags in the medicine-chest drawer bottom and most RFID tags in the drawer top can be read. Conclusion The multi-drawer coil antenna designed can effectively recognize the RFID tags in medicine-chest. It has a wide application prospect.

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