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1.
Eur J Clin Microbiol Infect Dis ; 35(9): 1475-8, 2016 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-27259711

RESUMEN

We evaluated two fully-automated real-time PCR systems, the novel QIAGEN artus MRSA/SA QS-RGQ and the widely used BD MAX MRSA assay, for their diagnostic performance in MRSA admission screening in a tertiary-care university hospital. Two hundred sixteen clinical swabs were analyzed for MRSA DNA using the BD MAX MRSA assay. In parallel, the same specimens were tested with the QIAGEN artus MRSA/SA QS-RGQ. Automated steps included lysis of bacteria, DNA extraction, real-time PCR and interpretation of results. MRSA culture was additionally performed as a reference method for MRSA detection. Sensitivity values were similar for both assays (80 %), while the QIAGEN artus MRSA/SA QS-RGQ reached a slightly higher specificity (95.8 % versus 90.0 %). Positive (PPVs) and negative predictive values (NPVs) were 17.4 % and 99.4 % for the BD MAX MRSA assay and 33.3 % and 99.5 % for the QIAGEN artus MRSA/SA QS-RGQ, respectively. Total turn-around time (TAT) for 24 samples was 3.5 hours for both assays. In conclusion, both assays represent reliable diagnostic tools due to their high negative predictive values, especially for the rapid identification of MRSA negative patients in a low prevalence MRSA area.


Asunto(s)
Automatización de Laboratorios/métodos , Portador Sano/diagnóstico , Pruebas Diagnósticas de Rutina/métodos , Tamizaje Masivo/métodos , Staphylococcus aureus Resistente a Meticilina/aislamiento & purificación , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Infecciones Estafilocócicas/diagnóstico , Portador Sano/microbiología , Humanos , Staphylococcus aureus Resistente a Meticilina/genética , Estudios Prospectivos , Sensibilidad y Especificidad , Infecciones Estafilocócicas/microbiología , Centros de Atención Terciaria , Factores de Tiempo
2.
Vox Sang ; 108(3): 314-7, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25471358

RESUMEN

In order to generate standardized conditions for the microbiological control of HPCs, the PEI recommended defined steps for validation that will lead to extensive validation as shown in this study, where a possible validation principle for the microbiological control of allogeneic SCPs is presented. Although it could be demonstrated that automated culture improves microbial safety of cellular products, the requirement for extensive validation studies needs to be considered.


Asunto(s)
Técnicas de Cultivo de Célula/normas , Guías como Asunto , Células Madre Hematopoyéticas/microbiología , Técnicas de Cultivo de Célula/métodos , Células Cultivadas , Alemania , Humanos
3.
Infection ; 43(2): 241-4, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25491170

RESUMEN

Mycoplasma hominis is a commensal of the genitourinary tract, which is infrequently associated with urogenital infections. Extra-urogenital infections due to M. hominis are rare. Here, we report an unusual case of M. hominis subdural empyema in a woman occurring shortly after delivery. The patient presented with symptoms suggestive of bacterial meningitis. Spinal imaging revealed a subdural empyema that required neurosurgical intervention. Cultures from intraoperatively obtained biopsies identified M. hominis as the causative pathogen. The patient was treated with oral moxifloxacin for 4 weeks resulting in the resolution of the spinal lesion. The subdural empyema was presumably caused by a contaminated epidural blood patch performed with the patient's own blood during an episode of transient M. hominis bacteremia after delivery. The blood patch was indicated for the treatment of cerebrospinal fluid leakage, which had occurred after epidural anesthesia. Our findings highlight the significance of transient M. hominis bacteremia after delivery and implicate that M. hominis should be considered as a causative agent of extra-genitourinary tract infections particularly during the postpartum period or after genitourinary manipulation.


Asunto(s)
Empiema Subdural/diagnóstico , Empiema Subdural/microbiología , Infecciones por Mycoplasma/diagnóstico , Infecciones por Mycoplasma/microbiología , Mycoplasma hominis , Periodo Posparto , Adulto , Antibacterianos/uso terapéutico , Femenino , Humanos , Imagen por Resonancia Magnética , Resultado del Tratamiento
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