RESUMEN
Recent advancements in ultra-sensitive detection, particularly the Aggregation Induced Emission (AIE) materials, have demonstrated a promising detection method due to their low cost, real-time detection, and simplicity of operation. Here, coumarin functionalized pillar[5]arene (P5C) and bis-bromohexyl pillar[5]arene (DP5) were successfully combined to create a linear AIE supramolecular pseudorotaxane polymer (PCDP-G). The use of PCDP-G as a supramolecular AIE polymer material for recyclable ultra-sensitive Fe3+ and F- detection is an interesting application of the materials. According to measurements, the low detection limits of PCDP-G for Fe3+ and F- are 4.16 × 10-10 M and 6.8 × 10-10 M, respectively. The PCDP-G is also a very effective logic gate and a material for luminous displays.
RESUMEN
To learn the current situation and strengthen the management of national standards for Chinese medicinal materials, we sorted out the relevant national standards. According to incomplete statistics, there are 1 185 kinds of Chinese medicinal materials, including 1 024 kinds of plant medicines, 106 kinds of animal medicines, and 54 kinds of mineral medicines, in addition to ethnic medicinal materials with different functions. The relevant standards include 819 Pharmacopoeia standards, 342 standards issued by the Ministry of Health or National Medicinal Products Administration, 7 standards for new medicinal materials, and 17 standards for imported medicinal materials. In this paper, the sources of standards as well as the distribution of families and genera and the distribution of medicinal parts of medicinal materials are analyzed. The suggestions are as follows:(1)to improve the coordination among different national standards of Chinese medicinal materials;(2)to improve the standardization and controllability of relevant standards;(3)to revise the issued standards for Chinese medicinal materials(including Tibetan, Uygur, and Mongolian medicinal materials).
Asunto(s)
Medicamentos Herbarios Chinos , Plantas Medicinales , Animales , Pueblo Asiatico , China , Humanos , Medicina Tradicional China , Estándares de ReferenciaRESUMEN
A novel water-soluble polysaccharide (named ALP-1) was successfully isolated from the stem barks of Acanthopanax leucorrhizus by hot-water extraction, and further purified by Cellulose DEAE-52 and Sephadex G-100 chromatography. The structure of ALP-1 was characterized by HPLC, HPGPC, partial acid hydrolysis, periodate oxidation, Smith degradation, methylation, together with UV, IR and NMR spectral analysis. The antioxidant activities also were evaluated in vitro. Structural analysis revealed that ALP-1 was a homogeneous galactan with the average molecular weight of 169â¯kDa, composed of galactose, glucose, mannose and arabinose in a molar ratio of 6.1:2.1:1.1:1.0, owning a backbone structure of 1,6-linked α-d-Galp residues with some branches of α-d-Manp-(1â¯ââ¯3)-α-l-Araf residues at O-3 and α-d-Galp residues at O-4 of 1,6-linked α-d-Galp. Antioxidant assay showed that ALP-1 exhibited strong DPPH and HO scavenging activities, as well as ferric-reducing antioxidant power. These results provide a scientific basis for the further use of polysaccharides from A. leucorrhizus.
RESUMEN
Twenty natural stilbenoids (1-20), including seven new stilbenoids (2, 4-7, 19, 20) and thirteen known stilbenoids (1, 3, 8-18), were isolated from the stem barks of Acanthopanax leucorrhizus, and six modified stilbenoid derivatives (1a, 2a, 4a, 4b, 7a and 17a) were obtained via methylation, demethylation and isopentenylation of the corresponding isolates (1, 2, 4, 7 and 17). These stilbenoids were structurally characterized by comprehensive analysis of their spectroscopic data and comparison with literature information, and evaluated for their cytotoxic activities against three human tumor cell lines (leukemia HL-60, hepatoma SMMC-7721 and breast carcinoma MCF-7) in vitro by MTT assay. The results showed that compounds 1a, 4a and 4b showed potent selective cytotoxicity against SMMC-7721 (IC50=10.16±1.95µM and 9.76±1.32µM) and MCF-7 (IC50=10.72±2.78µM) cell lines. The cytotoxic evaluation of these structurally modified stilbenoid derivatives have led to the establishment of a structure-activity relationship.
Asunto(s)
Antineoplásicos Fitogénicos/aislamiento & purificación , Eleutherococcus/química , Corteza de la Planta/química , Estilbenos/aislamiento & purificación , Línea Celular Tumoral , Humanos , Estructura Molecular , Relación Estructura-ActividadRESUMEN
A new cis-stilbenoid, 1,9-dihydroxy-10-methoxy-6H-dibenzo[b,f]oxocin-6-one (2) was isolated from the AcOEt extract of the stem barks of Acanthopanax leucorrhizus, along with three known stilbenoids, 9-hydroxy-10-methoxy-6H-dibenzo[b,f]oxocin-6-one (1), 5-O-methyl-(E)-resveratrol 3-O-ß-d-glucopyranoside (3), and (E)-resveratrol 3-O-ß-d-xylopyranoside (4). Two derivatives (2a and 2b) were synthesized by the structural modification of compound 2, which exhibited certain cytotoxic activities against HT-29 and HeLa cell lines in vitro. All compounds were structurally characterized by comprehensive analysis of their spectroscopic data and comparison with literature information, and evaluated for their cytotoxic activities against three human tumor cell lines (HL-60, HT-29, and HeLa) by the standard MTT assay in vitro. The results showed that derivatives 2a and 2b exhibited strong activities than compounds 2 against HT-29 and HeLa cell lines.
Asunto(s)
Eleutherococcus/química , Estilbenos/química , Supervivencia Celular/efectos de los fármacos , Eleutherococcus/metabolismo , Células HL-60 , Células HT29 , Células HeLa , Humanos , Espectroscopía de Resonancia Magnética , Conformación Molecular , Corteza de la Planta/química , Corteza de la Planta/metabolismo , Extractos Vegetales/química , Tallos de la Planta/química , Tallos de la Planta/metabolismo , Estilbenos/aislamiento & purificación , Estilbenos/toxicidadRESUMEN
This study was designed to explore the impact of depression on kidney-yang deficiency in rats. Rats were repeatedly injected with hydrocortisone for 21 days to establish the depression model with kidneyyang deficiency. Tolbutamide, chlorzoxazone, theophylline, midazolam, omeprazole and dextromethorphan were used as substrates of CYP2C6, CYP2E1, CYP1A2, CYP3A2, CYP2D1, and CYP2D2 to test the depression impact on drug metabolism. Plasma concentrations of six CYP450 were determined by LC-MS/MS and used as pharmacokinetic parameters. Consequently, metabolism of theophylline, chlorzoxazone and tolbutamide were accelerated significantly in the model relative to the control (P < 0.01), but dextromethorphan, omeprazole and midazolam did not exhibit a significant difference. The present study suggests that depression with kidneyyang deficiency had a strong induction of CYP2E1 and moderate induction of CYP1A2, CYP2C6 in the rat model.
Asunto(s)
Sistema Enzimático del Citocromo P-450/metabolismo , Depresión/enzimología , Hígado/enzimología , Deficiencia Yang , Animales , Clorzoxazona , Cromatografía Liquida , Dextrometorfano , Hidrocortisona , Midazolam , Omeprazol , Ratas , Espectrometría de Masas en Tándem , Teofilina , TolbutamidaRESUMEN
In order to reaction the quality present situation, problems on the current quality of animal sources of drugs are summed up by using test data analysis, literature search and marketing research. This paper can also help the improvement of the quality management, the revision of the relevant department policy system and the improvement of standards.
Asunto(s)
Medicina Tradicional China/normas , Preparaciones Farmacéuticas/normas , Animales , China , Preparaciones Farmacéuticas/análisis , Control de CalidadRESUMEN
Four diterpenoids, including two new ones, namely noracanthopanin A (1) and acanthopanolide B (2), and two known ones, ent-pimara-9(11),15-dien-19-oic acid (3) and ent-kaur-16-en-19-oic acid (4), were isolated from the stem bark of Acanthopanax brachypus Harms. Their structures were elucidated by physicochemical and spectroscopic analyses. Compound 1 and 2 exhibited moderate cytotoxicities against HO-8910 cells with IC50 values of 65.21±0.23 and 31.43±1.01â µg/ml, respectively, and 2 showed selective weak cytotoxicity against SMMC-7721 cells (IC50 89.67±2.54â µg/ml).
Asunto(s)
Diterpenos/química , Eleutherococcus/química , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Diterpenos/aislamiento & purificación , Diterpenos/toxicidad , Eleutherococcus/metabolismo , Humanos , Corteza de la Planta/química , Corteza de la Planta/metabolismo , Tallos de la Planta/química , Tallos de la Planta/metabolismoRESUMEN
A new diterpene glycoside, tomentoside I (1), along with eleven known compounds, including the four coumarins, 4,5-dimethoxyl-7-methylcoumarin (2), 4,7-dimethoxyl-5-methylcoumarin (3), isofraxidin (4) and fraxidin (5) as well as the seven triterpenoids, oleanolic acid (6), oleanolic acid 3-O-α-L-arabinopyranoside (7), oleanolic acid 3-O-ß-D-galactopyranosyl-(1â3)-ß-D-glucopyranoside (8), hederagenin 3-O-α-L-arabinopyranoside (9), betulinic acid (10), 18-hydroxyursolic acid (11) and 2α,3ß,23-trihydroxyurs-12-en-28-oic acid (12) were isolated from the ethanolic extract of the root of Anemone tomentosa and their chemical structures were elucidated by spectroscopic methods. The antimicrobial activities of compounds 1-12 were measured using the agar disc-diffusion method. Also, their antioxidant activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) were evaluated.
Asunto(s)
Anemone/metabolismo , Medicamentos Herbarios Chinos/farmacología , Raíces de Plantas/química , Anemone/química , Cumarinas/síntesis química , Cumarinas/química , Cumarinas/farmacología , Medicamentos Herbarios Chinos/química , Depuradores de Radicales Libres/síntesis química , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/aislamiento & purificación , Depuradores de Radicales Libres/farmacología , Glicósidos/síntesis química , Glicósidos/química , Glicósidos/aislamiento & purificación , Glicósidos/farmacología , Pruebas de Sensibilidad Microbiana , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/química , Ácido Oleanólico/aislamiento & purificación , Ácido Oleanólico/farmacología , Triterpenos Pentacíclicos , Fitosteroles/síntesis química , Fitosteroles/química , Fitosteroles/farmacología , Fitoterapia , Saponinas/química , Saponinas/aislamiento & purificación , Saponinas/farmacología , Esteroles/química , Esteroles/aislamiento & purificación , Esteroles/farmacología , Relación Estructura-Actividad , Triterpenos/síntesis química , Triterpenos/química , Triterpenos/aislamiento & purificación , Triterpenos/farmacología , Ácido BetulínicoRESUMEN
Two new natural products, (3R)-5,7-dihydroxy-8-(2â³-O-veratroyl-ß-D-glucopyranosyl)-3-(4'-hydroxyphenyl)-6-methylchroman-4-one (1) and 4'-hydroxyisoflavone-7-O-[α-L-arabinopyranosyl-(1â6)-ß-D-glucopyranoside] (2), along with eleven other known flavonoids, were isolated from the roots of Acanthopanax brachypus. Their structures were elucidated on the basis of spectroscopic and chemical evidence.
Asunto(s)
Eleutherococcus/química , Flavonoides/química , Flavonoides/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Conformación Molecular , Raíces de Plantas/químicaRESUMEN
Two new triterpenoid saponins, brachyposide A (1) {3-O-beta-D-galactopyranosyl-(1-->2)-[beta-D-xylopyranosyl-(1-->3)]-beta-D-glucuronopyranosyl-2beta,3beta,16alpha,23-tetrahydroxyolean-12-en-28-oic acid 28-O-beta-D-apiofuranosyl-(1-->3)-beta-D-xylopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranosyl ester} and brachyposide B (2) {3-O-beta-D-glucopyranosyl-(1-->3)-beta-D-galactopyranosyl-(1-->3)-beta-D-glucuronopyranosyl-2beta,3beta,23-trihydroxyolean-12-en-28-oic acid 28-O-beta-D-xylopyranosyl-(1-->4)-[beta-D-apiofuranosyl-(1-->3)]-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranosyl ester}, together with four known triterpenoid saponins, including tabguticoside A, nipponoside D, palmatoside E and ciwujianoside A(1), were isolated from the stem bark of Acanthopanax brachypus. Their structures were elucidated on the basis of spectroscopic and chemical evidence.
Asunto(s)
Eleutherococcus/química , Saponinas/química , Triterpenos/química , Espectroscopía de Resonancia Magnética , Tallos de la Planta/química , Saponinas/aislamiento & purificación , Triterpenos/aislamiento & purificaciónRESUMEN
OBJECTIVE: The chemical constituents and antimicrobial activity of the essential oil from Acanthopanax brachypus were studied. METHODS: The essential oil was extracted from the stem of A. brachypus by steam distillation, and its antimicrobial activity was tested in vitro. The chemical constituents were separated and identified by GC-MS, and the relative contents of each constituent was determined by area normalization. RESULTS: The essential oil showed some certain antibacterial activities against the tested strains escherichia coli, bacillus subtilis, staphylococcus aureus, pseudomonas aeruginosa and candida albicans except aspergillus niger. Forty-seven constituents were separated and identified, accounting for 91.37% of the total oil. The main constituent were Heptanoic acid (7.05%), Vanillin (6.09%), trans-Linalool oxide (6.07%), 1-methyl-2-(-methylethyl)-Benaene (5.83%), alpha-Phellandrene (5.14%), n-Hexadecanoic acid (5.15%) and beta-Myrcene (5.07%). CONCLUSION: The essential oil of A. brachypus contained varied active constituent, has a certain antimicrobial activity, this result will provide some scientific references for the pharmacological further research of A. brachypus.
Asunto(s)
Eleutherococcus/química , Ácidos Heptanoicos/análisis , Aceites Volátiles/aislamiento & purificación , Aceites Volátiles/farmacología , Plantas Medicinales/química , Antibacterianos/química , Antibacterianos/farmacología , Benzaldehídos/análisis , Monoterpenos Ciclohexánicos , Escherichia coli/efectos de los fármacos , Cromatografía de Gases y Espectrometría de Masas , Pruebas de Sensibilidad Microbiana , Monoterpenos/análisis , Aceites Volátiles/química , Ácidos Palmíticos/análisis , Tallos de la Planta/química , Staphylococcus aureus/efectos de los fármacosRESUMEN
OBJECTIVE: To study the chemical constituents from the aerial parts of Polygomun aviculane. METHOD: The chemical constituents were isolated by silica gel column chromatography and preparative silica thin layer chromatography, and their structures were elucidated on the basis of physico-chemical evidences and spectroscopic analysis (IR, MS, 1H and 13C-NMR). RESULT: Seven phenolic compounds were identified as rosmarinic acid (1), gallic acid (2), gentisic acid 5-O-(6'-O-galloyl)-beta-D-glucopyranoside (3), caffeic acid (4), p-coumaric acid (5), ethyl caffeate (6) and acteoside (7), respectively. CONCLUSION: Compounds 1, 3, 6 and 7 were isolated from this plant for the first time. These results provided theoretical evidences for the further bioactive investigation on this plant.
Asunto(s)
Ácidos Cafeicos/aislamiento & purificación , Cinamatos/aislamiento & purificación , Depsidos/aislamiento & purificación , Glucósidos/aislamiento & purificación , Fenoles/aislamiento & purificación , Plantas Medicinales/química , Polygonum/química , Ácidos Cafeicos/química , Cinamatos/química , Depsidos/química , Glucósidos/química , Fenoles/química , Componentes Aéreos de las Plantas/química , Ácido RosmarínicoRESUMEN
In the present paper, orthogonal test and square error analysis were applied to the determination of inorganic elements in the aerial part of E. bodinieri V. such as K, Na, Ca, Ba, Pb, Zn, Mn, Cu, Mg, Fe and Al by microwave digestion ICP-AES technique. The method is simple, rapid, and features of high sensitivity, good accuracy and simultaneous determination of many elements. Its recovery ratio obtained by standard addition method ranged from 93.2% to 104.1%, and its RSD (n=5) was lower than 3.20%. The results showed that this plant has rich inorganic elements, which are essential to human health. The result provides a theoretical basis for investigating the correlation of element content and the effect of medicine in E. bodinieri V.