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1.
Braz J Microbiol ; 46(3): 683-90, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26413048

RESUMEN

An extracellular ß-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg(2+), Mn(2+), K(+); Ca(2+), Na(+), Ba(2+), Zn(2+), Cu(2+), Co(2+), Fe(2+), Sr(2+) and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.


Asunto(s)
Adaptación Fisiológica/fisiología , Agar/metabolismo , Glicósido Hidrolasas/metabolismo , Pseudoalteromonas/enzimología , Adaptación Fisiológica/genética , Regiones Antárticas , Proteínas Bacterianas/metabolismo , Frío , Disacáridos/biosíntesis , Sedimentos Geológicos/microbiología , Glicósido Hidrolasas/aislamiento & purificación , Hidrólisis , ARN Ribosómico 16S/genética
2.
Braz. j. microbiol ; Braz. j. microbiol;46(3): 683-690, July-Sept. 2015. tab, ilus
Artículo en Inglés | LILACS | ID: lil-755831

RESUMEN

An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.

.


Asunto(s)
Adaptación Fisiológica/fisiología , Agar/metabolismo , Glicósido Hidrolasas/metabolismo , Pseudoalteromonas/enzimología , Regiones Antárticas , Adaptación Fisiológica/genética , Proteínas Bacterianas/metabolismo , Frío , Disacáridos/biosíntesis , Sedimentos Geológicos/microbiología , Glicósido Hidrolasas/aislamiento & purificación , Hidrólisis , /genética
3.
Braz. J. Microbiol. ; 46(3): 683-690, July-Sept. 2015. tab, ilus
Artículo en Inglés | VETINDEX | ID: vti-14832

RESUMEN

An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries..(AU)


Asunto(s)
Adaptación Fisiológica/fisiología , Agar/metabolismo , /metabolismo , Pseudoalteromonas/enzimología , Adaptación Fisiológica/genética , Regiones Antárticas , Proteínas Bacterianas/metabolismo , Frío , Disacáridos/biosíntesis , Sedimentos Geológicos/microbiología , Glicósido Hidrolasas/aislamiento & purificación , Hidrólisis , /genética
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