RESUMEN
In gestational diabetes mellitus (GDM), adipose tissue undergoes metabolic disturbances and chronic low-grade inflammation. Alternative polyadenylation (APA) is a post-transcriptional modification mechanism that generates mRNA with variable lengths of 3' untranslated regions (3'UTR), and it is associated with inflammation and metabolism. However, the role of APA in GDM adipose tissue has not been well characterized. In this study, we conducted transcriptomic and proteomic sequencing on subcutaneous and omental adipose tissues from both control and GDM patients. Using Dapars, a novel APA quantitative algorithm, we delineated the APA landscape of adipose tissue, revealing significant 3'UTR elongation of mRNAs in the GDM group. Omental adipose tissue exhibited a significant correlation between elongated 3'UTRs and reduced translation levels of genes related to metabolism and inflammation. Validation experiments in THP-1 derived macrophages (TDMs) demonstrated the impact of APA on translation levels by overexpressing long and short 3'UTR isoforms of a representative gene LRRC25. Additionally, LRRC25 was validated to suppress proinflammatory polarization in TDMs. Further exploration revealed two underexpressed APA trans-acting factors, CSTF3 and PPP1CB, in GDM omental adipose tissue. In conclusion, this study provides preliminary insights into the APA landscape of GDM adipose tissue. Reduced APA regulation in GDM omental adipose tissue may contribute to metabolic disorders and inflammation by downregulating gene translation levels. These findings advance our understanding of the molecular mechanisms underlying GDM-associated adipose tissue changes.
RESUMEN
As manipulating images by copy-move, splicing and/or inpainting may lead to misinterpretation of the visual content, detecting these sorts of manipulations is crucial for media forensics. Given the variety of possible attacks on the content, devising a generic method is nontrivial. Current deep learning based methods are promising when training and test data are well aligned, but perform poorly on independent tests. Moreover, due to the absence of authentic test images, their image-level detection specificity is in doubt. The key question is how to design and train a deep neural network capable of learning generalizable features sensitive to manipulations in novel data, whilst specific to prevent false alarms on the authentic. We propose multi-view feature learning to jointly exploit tampering boundary artifacts and the noise view of the input image. As both clues are meant to be semantic-agnostic, the learned features are thus generalizable. For effectively learning from authentic images, we train with multi-scale (pixel / edge / image) supervision. We term the new network MVSS-Net and its enhanced version MVSS-Net++. Experiments are conducted in both within-dataset and cross-dataset scenarios, showing that MVSS-Net++ performs the best, and exhibits better robustness against JPEG compression, Gaussian blur and screenshot based image re-capturing.
