RESUMEN
AIM: To study the mRNA levels of chemokine receptor CXCR1, CXCR2 and IL-8 in the patients with chronic hepatitis B and their association with IFN therapy. METHODS: The mRNA levels of CXCR1, CXCR2 and IL-8 in peripheral blood mononuclear cells(PBMC)of chronic hepatitis B patients were determined by real-time RT-PCR before and during the IFN-α therapy. RESULTS: The mRNA levels of CXCR1(0.4474 ± 0.0386)and IL-8(1.1897 ± 0.1028)in peripheral blood of the patients with chronic hepatitis B were significantly higher than those in healthy donors(P<0.01). The mRNA level of CXCR2(0.4720 ± 0.0458)was higher than those in healthy donors, but there was no significant differences between the two groups. During the treatment, the mRNA levels of CXCR1, CXCR2 and IL-8 obviously decreased. After treatment for six month, the mRNA level of CXCR1(0.4129 ± 0.0395), CXCR2(0.4461 ± 0.0477) and IL-8(0.8660 ± 0.1307)were significantly lower than those before treatment(P<0.01 or P<0.05). Additionally, the expressive levels of CXCR1, CXCR2 and IL-8 in the high HBV loading group(HBV-DNA>10(6);)were much higher than those in the low HBV loading group(HBV-DNA<10(6);). CONCLUSION: CXCR1 and IL-8 may contribute to hepatic inflammation. Among them, CXCR1, CXCR2 and IL-8 decrease after IFN treatment, which illustrates that IFN-α plays an important role in down-regulating inflammation response, controlling the development of the patients' condition.
Asunto(s)
Hepatitis B Crónica/tratamiento farmacológico , Interferones/uso terapéutico , Interleucina-8/genética , Receptores de Interleucina-8A/genética , Receptores de Interleucina-8B/genética , Adulto , Alanina Transaminasa/sangre , Antivirales/uso terapéutico , Células Cultivadas , ADN Viral/sangre , ADN Viral/genética , Femenino , Expresión Génica/efectos de los fármacos , Virus de la Hepatitis B/efectos de los fármacos , Virus de la Hepatitis B/genética , Hepatitis B Crónica/genética , Hepatitis B Crónica/virología , Humanos , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Factores de Tiempo , Resultado del Tratamiento , Carga Viral , Adulto JovenRESUMEN
AIM: To clone cDNAs of thrombin-like enzymes (TLEs) from venom gland of Deinagkistrodon acutus and analyze the mechanisms by which their structural diversity arose. METHODS: Reverse transcription-polymerase chain reaction and gene cloning techniques were used, and the cloned sequences were analyzed by using bioinformatics tools. RESULTS: Novel cDNAs of snake venom TLEs were cloned. The possibilities of post-transcriptional recombination and horizontal gene transfer are discussed. A phylogenetic tree was constructed. CONCLUSION: The cDNAs of snake venom TLEs exhibit great diversification. There are several types of structural variations. These variations may be attributable to certain mechanisms including recombination.