RESUMEN
Long-term and high-dose glucocorticoid treatment is recognized as an important influencing factor for osteoporosis and osteonecrosis. Nicotinamide mononucleotide (NMN) is an intermediate of NAD+ biosynthesis, and is widely used to replenish the levels of NAD+. However, the potential role of NMN in glucocorticoidinduced osteogenic inhibition remains to be demonstrated. In the present study, the protective effects of NMN on dexamethasone (Dex)induced osteogenic inhibition, and its underlying mechanisms, were investigated. Bone mesenchymal stem cells were treated with Dex, which decreased the levels of the osteogenic markers alkaline phosphatase, Runtrelated transcription factor 2 and osteocalcin. NMN treatment attenuated Dexinduced osteogenic inhibition and promoted the expression of sirtuin 1 (SIRT1) and peroxisome proliferatoractivated receptor gamma coactivator (PGC)1α. SIRT1 knockdown reversed the protective effects of NMN and reduced the expression levels of PGC1α. Collectively, the results of the present study reveal that NMN may be a potential therapeutic target for glucocorticoidinduced osteoporosis.