Clays and Wound Healing.

Aluminosilicates, such as montmorillonite, kaolinite, halloysite, and diatomite, have a uniform bidimensional structure, a high surface-to-volume ratio, inherent stiffness, a dual charge distribution, chemical inertness, biocompatibility, abundant active groups on the surface, such as silanol (Si-OH) and/or aluminol (Al-OH) groups. These compounds are on the list of U.S. Food and Drug Administration-approved active compounds and excipients and are used for various medicinal products, such as wound healing agents, antidiarrheals, and cosmetics. This review summarizes the wound healing mechanisms related to the material characteristics and the chemical components. Numerous wound dressings with different active components and multiple forms have been studied. Then, medicinal mineral resources for use in hemostatic materials can be developed.

Functional ß-TCP/MnO2 /PCL artificial periosteum promoting osteogenic differentiation of BMSCs by reducing locally reactive oxygen species level.

Segmental bone defects caused by trauma, tumor resection or congenital malformations are often reconstructed with autologous, allogeneic bone grafts or artificial bone materials, of which, about 5% ~ 10% will have delayed healing or even nonunion of fractures. The loss of periosteum and excessive accumulation of ROS in fracture site leads to the aging of osteoblasts and the decline of their proliferation and differentiation, thus affecting the fracture healing process. In this study, we prepared a functional modified artificial periosteum ß-TCP/MnO2 /PCL(ß-TMP) by electrospinning with a function of catalyzing decomposition of H2 O2 . We examined the surface morphology of ß-TMP, the concentration of Ca, P and Mn of ß-TMP, as well as the diameter distribution range of nanofibers on ß-TMP. Through X-ray diffraction patterns and Fourier transform infrared spectra, ß-TMP was characterized and its hydrophilicity was tested. The release of Mn2+ and Ca2+ of 0.1 and 0.05% ß-TMP in different pH values (7.4 and 5.5) determined by ICP. We also identified that ß-TMP could reduce the level of ROS in cells by lowering the level of H2 O2 . 0%, 0.05% and 0.1% ß-TMP displayed good cell compatibility, cell adhesion and cellular morphology in the condition with or without H2 O2 . 0.5% ß-TMP showed compromised cell compatibility in normal condition, however, the compromised phenotypes could be partially rescued in the present of H2 O2 . Compared with 0%, 0.05% and 0.1% ß-TMP displayed higher osteoblastic differentiation with or without H2 O2 in BMSCs as well as in MG-63. In sum, ß-TMP helped osteogenesis and promoted repair of bone defects.

Robust hemostatic bandages based on nanoclay electrospun membranes.

Death from acute hemorrhage is a major problem in military conflicts, traffic accidents, and surgical procedures, et al. Achieving rapid effective hemostasis for pre-hospital care is essential to save lives in massive bleeding. An ideal hemostasis material should have those features such as safe, efficient, convenient, economical, which remains challenging and most of them cannot be achieved at the same time. In this work, we report a rapid effective nanoclay-based hemostatic membranes with nanoclay particles incorporate into polyvinylpyrrolidone (PVP) electrospun fibers. The nanoclay electrospun membrane (NEM) with 60 wt% kaolinite (KEM1.5) shows better and faster hemostatic performance in vitro and in vivo with good biocompatibility compared with most other NEMs and clay-based hemostats, benefiting from its enriched hemostatic functional sites, robust fluffy framework, and hydrophilic surface. The robust hemostatic bandages based on nanoclay electrospun membrane is an effective candidate hemostat in practical application.

Electrospun polycaprolactone/hydroxyapatite/ZnO films as potential biomaterials for application in bone-tendon interface repair.

The bone-tendon interface (BTI) is a graded structure consisting of bone, mineralized and nonmineralized fibrocartilage, and tendons. Due to the complexity of the BTI structure, BTI healing is particularly challenging. To achieve a better material for BTI healing, polycaprolactone (PCL)/hydroxyapatite (HA)/ZnO films were constructed by the electrospinning method; in addition, the relevant material characteristics were tested. After culturing MC3T-E1 cells, ATDC5 cells, mouse primary fibrochondrocytes, and mouse primary tenocytes on films, PCL-5%HA-1%ZnO films (HA and ZnO weight ratios of 5% and 1%, respectively) displayed superior cell compatibility and cell adhesion. PCL-5%HA-1%ZnO films also promoted osteogenesis, chondrogenesis, fibrocartilage formation, and tendon healing. The antibacterial characteristics of PCL-5%HA-1%ZnO films were also identified in this study. The PCL-5%HA-1%ZnO films have great application potential in the field of BTI repair.