Squamous cell carcinoma of the anal sac in five dogs.

Tumors of the perianal area of dogs are common and include multiple tumor types. Whereas perianal adenomas occur often, adenocarcinomas of the apocrine glands of the anal sac occur less frequently. A review of the literature revealed no reports of squamous cell carcinomas arising from the epithelial lining of the anal sac. Squamous cell carcinomas originating from the lining of the anal sac were diagnosed in five dogs. Microscopically, the tumors consisted of variably sized invasive nests and cords of epithelial cells displaying squamous differentiation. Four of the five dogs were euthanatized because of problems associated with local infiltration by the tumors. In the fifth dog, there was no evidence of tumor 7 months after surgical removal, but further follow up was not available.

Monoclonal antibodies to conformational epitopes of the surface glycoprotein of caprine arthritis-encephalitis virus: potential application to competitive-inhibition enzyme-linked immunosorbent assay for detecting antibodies in goat sera.

Four immunoglobulin G1 monoclonal antibodies (MAbs) to the gp135 surface envelope glycoprotein (SU) of the 79-63 isolate of caprine arthritis-encephalitis virus (CAEV), referred to as CAEV-63, were characterized and evaluated for their ability to compete with antibody from CAEV-infected goats. Three murine MAbs (MAbs GPB16A, 29A, and 74A) and one caprine MAb (MAb F7-299) were examined. All MAbs reacted in nitrocellulose dot blots with native CAEV-63 SU purified by MAb F7-299 affinity chromatography, whereas none reacted with denatured and reduced SU. All MAbs reacted in Western blots with purified CAEV-63 SU or the SU component of whole-virus lysate following denaturation in the absence of reducing agent, indicating that intramolecular disulfide bonding was essential for epitope integrity. Peptide-N-glycosidase F digestion of SU abolished the reactivities of MAbs 74A and F7-299, whereas treatment of SU with N-acetylneuraminate glycohydrolase (sialidase A) under nonreducing conditions enhanced the reactivities of all MAbs as well as polyclonal goat sera. MAbs 29A and F7-299 were cross-reactive with the SU of an independent strain of CAEV (CAEV-Co). By enzyme-linked immunosorbent assay (ELISA), the reactivities of horseradish peroxidase (HRP)-conjugated MAbs 16A and 29A with homologous CAEV-63 SU were <10% of that of HRP-conjugated MAb 74A. The reactivity of HRP-conjugated MAb 74A was blocked by sera from goats immunized with CAEV-63 SU or infected with CAEV-63. The reactivity of MAb 74A was also blocked by sera from goats infected with a CAEV-Co molecular clone, although MAb 74A did not react with CAEV-Co SU in Western blots. Thus, goats infected with either CAEV-63 or CAEV-Co make antibodies that inhibit binding of MAb 74A to CAEV-63 SU. A competitive-inhibition ELISA based on displacement of MAb 74A reactivity has potential applicability for the serologic diagnosis of CAEV infection.

Effects of macrophage inhibitory factor-A3 (MIF-A3) on cytokine secretion and phagolysosome fusion in murine macrophages.

Macrophage inhibitory factor-A3 (MIF-A3) is a fraction derived from Mycobacterium avium serovar 2 (Mav2) that consists of a small amine containing compound (peptide), trehalose and two or three short chain fatty acids. MIF-A3 has been shown to inhibit candidacidal activity of murine thioglycolate-elicited peritoneal-derived macrophages and bovine peripheral blood monocytes, and scavenge reactive oxygen intermediates. In this study, MIF-A3 was evaluated for its effect on secretion of IL-1beta, IL-6, IL-10, TNFalpha and GM-CSF in C57BL/6 murine thioglycolate-elicited peritoneal-derived macrophages, with and without pre-incubation with affinity purified goat anti-MIF-A3 IgG, using ELISA cytokine kit analysis. Results of this study suggest that anti-MIF-A3 IgG does not enhance clearance of Mav2, alter phagocytosis or alter phagosome-lysosome interactions as determined by electron microscopy in Mav2 infected macrophages. MIF-A3 does induce secretion of IL-6, but does not induce secretion of TNFalpha, IL-1beta, and GM-CSF. TNFalpha has been previously shown to reduce growth, while IL-6 has been shown to enhance growth of M. avium. Since IL-6 appears to enhance growth of M. avium and MIF-A3 induces IL-6 secretion, MIF-A3 may be responsible for enhanced intracellular growth in M. avium infections and be a factor in the pathogenesis of M. avium infections.

Multiple persistent vitelline duct cysts in a dog.

Persistent vitelline duct remnants, with the exception of Meckel's diverticulum in pigs and horses, are rare in animals. During an ovariohysterectomy of an 8-month-old Labrador Retriever, multiple fibrous nodules with cystic centers were found attached to the ileal serosa and in a mesodiverticular band attached to the abdominal wall. Histologic and ultrastructural evaluation revealed that the cysts were composed of well-differentiated intestine with mucosa, submucosa, and muscularis layers surrounded by a thick layer of fibrous connective tissue. The morphology and arrangement of lesions were consistent with multiple persistent vitelline duct cysts, a distinct condition related to Meckel's diverticulum. This case in a dog represents a unique presentation of this congenital anomaly in domestic animals.

Depressed CD4+ T lymphocyte proliferative response and enhanced antibody response to viral antigen in chronic lentivirus-induced arthritis.

The proliferative response of peripheral blood mononuclear cells (PBMC) stimulated with caprine arthritis-encephalitis virus (CAEV) surface glycoprotein (SU) was depressed 4- to 20-fold in Saanen goats with chronic CAEV-induced arthritis compared with asymptomatic goats. Phytohemagglutinin-stimulated responses were not depressed. Complement depletion of PBMC with anti-CD4 or anti-CD8 monoclonal antibodies identified CD4+ T lymphocytes as the antigen-responsive cells in both high-responder asymptomatic goats and low-responder arthritic goats. Serum antibody titers to CAEV SU were 8- to 32-fold higher in goats with chronic arthritis. Increased anti-CAEV SU titers as early as 3 months after infection predicted the eventual development of clinical arthritis. Thus, CAEV-induced arthritis is associated with chronic B cell activation resulting from dominant type 2 immune responses to viral antigen. The clinical outcome of CAEV infection may be determined by differential activation of type 1 or 2 T lymphocyte phenotypes at or near the time of initial exposure to CAEV.

Caprine arthritis-encephalitis lentivirus (CAEV) challenge of goats immunized with recombinant vaccinia virus expressing CAEV surface and transmembrane envelope glycoproteins.

This study evaluated infection and disease following caprine arthritis-encephalitis lentivirus (CAEV) challenge of goats with existent immune response to CAEV surface and transmembrane envelope glycoproteins. Six Saanen goats were vaccinated three times with recombinant vaccinia virus rWR63 expressing glycoproteins encoded by the CAEV-63 envelope gene. Two goats were immunized with rWRSC11, a control vaccinia virus derived from the pSC11 vaccinia expression plasmid without the CAEV envelope gene. One pair of rWR63 vaccinated goats received a booster immunization with recombinant surface glycoprotein in Freund's complete adjuvant, a second pair was boosted by intravenous inoculation with rWR63, and the third pair was boosted by immunization with HPLC purified native CAEV surface glycoprotein in Freund's complete adjuvant. All six goats vaccinated with rWR63 developed antibody responses to CAEV envelope glycoproteins; however, CAEV-63 neutralizing antibody was not detected. Neither of the rWRSC11-vaccinated goats developed CAEV reactive antibody. All goats were challenged by intravenous inoculation with 10(6) TCID50 CAEV-63. All goats became infected following challenge infection, shown by detection of serum antibody to CAEV core proteins and virus isolation. Existent CAEV-63 immune responses did not detectably alter the severity of inflammatory joint lesions at 24 weeks postchallenge.

Caprine arthritis-encephalitis lentivirus SU is the ligand for infection of caprine synovial membrane cells.

Infection of goat synovial membrane (GSM) cells by caprine arthritis-encephalitis virus (CAEV) was inhibited by incubation of cells with CAEV gp 135 envelope glycoprotein (SU) expressed by recombinant vaccinia virus. Incubation of cells with protein expressed by a control recombinant vaccinia virus without the CAEV envelope gene did not inhibit CAEV infection. Removal of recombinant SU from blocking medium by adsorption with anti-SU IgG/protein G-Sepharose complexes resulted in loss of CAEV inhibition. Results support that CAEV infection of GSM cells is mediated by a specific interaction between SU and a cell surface receptor or receptor complex.

Squamous cell carcinoma in a free-ranging bighorn sheep (Ovis canadensis californiana).

An oral squamous cell carcinoma which invaded maxillary bones with metastasis to the right retropharyngeal lymph node was diagnosed in a free-ranging California bighorn sheep (Ovis canadensis californiana) from Washington. Much of the maxillae had been replaced with tumor and reactive tissue, and many teeth were missing or loose. The tumor was predominantly confined to the shape of the maxillary bones and was unusual because it was bilaterally symmetrical.