RESUMEN
Parkinson's disease (PD) is characterized by selective degeneration of neurons in the substantia nigra and subsequent dysfunction of dopaminergic neurotransmission. Genes identified in familial forms of PD encode proteins that are linked to the ubiquitin-proteasome system indicating the pathogenic relevance of disturbed protein degradation in PD. Some of them, i.e. alpha-synuclein, parkin and synphilin-1, have been implicated in presynaptic neurotransmission based on their localization in synaptic vesicles. Synaptotagmin XI is linked to the pathogenesis of PD based on its identification as a substrate of the ubiquitin-E3-ligase parkin. Moreover synaptotagmin XI is involved in the maintainance of synaptic function and represents a component of Lewy bodies (LB) in brains of PD patients. Therefore, we performed a detailed mutation analysis of the synaptotagmin XI gene in a large sample of 393 familial and sporadic PD patients. We did not find any disease causing mutations arguing against a major role of mutations in the synaptotagmin XI gene in the pathogenesis of PD.
Asunto(s)
Proteínas de Unión al Calcio/genética , Pruebas Genéticas/métodos , Glicoproteínas de Membrana/genética , Mutación/genética , Proteínas del Tejido Nervioso/genética , Enfermedad de Parkinson/genética , Anciano , Secuencia de Aminoácidos/genética , Secuencia de Bases/genética , Femenino , Humanos , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Polimorfismo de Nucleótido Simple/genética , Análisis de Secuencia de ADN/métodos , SinaptotagminasRESUMEN
Mutations of the parkin gene are a cause of autosomal recessive juvenile parkinsonism. Although the parkin gene has been isolated from mouse, rat, and human, little is known about its expression in neural and nonneural tissues during development. In this study, we used a polyclonal antibody to a peptide downstream of the parkin ubiquitin domain to investigate (1) the differential expression of parkin isoforms in protein extracts from fetal and adult mouse tissues, and (2) the distribution of parkin in mouse fetal tissues at different developmental stages and in adult CNS tissues. By Western blot analyses, at least three isoforms of parkin of 22, 50, and 55 kDa were differentially expressed in mouse tissues. The p22 and p50 isoforms were found in fetal and adult mouse CNS tissues, while the p55 isoform was found only in adult tissues. The p50 isoform is the predominant form in both fetal and adult tissues. Immunolocalization in mouse fetuses showed that parkin was expressed only after neuronal differentiation. Although parkin was localized throughout the cytoplasm, the highest level of parkin was found in the neurites of both fetal and adult neurons.
Asunto(s)
Encéfalo/embriología , Encéfalo/metabolismo , Ligasas/biosíntesis , Trastornos Parkinsonianos/metabolismo , Ubiquitina-Proteína Ligasas , Factores de Edad , Animales , Anticuerpos , Química Encefálica , Dopamina/fisiología , Epítopos/inmunología , Inmunohistoquímica , Isomerismo , Ligasas/química , Ligasas/inmunología , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos DBA , ConejosRESUMEN
Human ataxin-2 contains a polyglutamine repeat that is expanded in patients with spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is highly conserved in evolution with orthologs in mouse, Caenorhabditis elegans, and Drosophila melanogaster. It interacts at its C-terminus with ataxin-2 binding protein 1, A2BP1. This study presents a highly conserved mouse ortholog of A2BP1, designated A2bp1. The amino acid sequence of the human and mouse protein is 97.6% identical. This remarkable degree of conservation supports the fact that these proteins have an important basic function in development and differentiation. Sequence analysis reveals the existence of RNA binding motifs. The A2bp1 transcript was found in various regions of the CNS including cerebellum, cerebral cortex, brain stem, and thalamus/hypothalamus. The A2bp1 protein was detected by immunocytochemistry in the CNS and connective tissue of the mouse embryo starting at stage E11, as well as in the heart at all stages. Mouse embryos showed varying expression of A2bp1 at all stages. Previous studies in other model systems had implicated the orthologs of ataxin-2 and A2BP1 in development. This study suggests a role for A2bp1 in embryogenesis as well as in the adult nervous system, possibly mediated by a function in RNA distribution or processing.
Asunto(s)
Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Animales , Ataxinas , Proteínas Portadoras/química , Cromosomas/genética , Clonación Molecular , Secuencia Conservada , Embrión de Mamíferos/metabolismo , Regulación del Desarrollo de la Expresión Génica , Biblioteca Genómica , Humanos , Ratones , Modelos Animales , Datos de Secuencia Molecular , Proteínas del Tejido Nervioso , Especificidad de Órganos , Filogenia , Proteínas/genética , Células de Purkinje/metabolismo , Factores de Empalme de ARN , ARN Mensajero/genética , ARN Mensajero/metabolismo , Proteínas de Unión al ARN , Mapeo de Híbrido por Radiación , Homología de Secuencia de AminoácidoRESUMEN
BACKGROUND: Early-onset torsion dystonia is a hyperkinetic movement disorder caused by a deletion of 1 glutamic acid residue in torsin A protein, a novel member of the AAA family of adenosine triphosphatases. No mutation has been found so far in the closely related torsin B protein. Little is known about the molecular basis of the disease, and the cellular functions of torsin proteins remain to be investigated. OBJECTIVE: To study the regional, cellular, and subcellular distribution of the torsin A and torsin B proteins. METHODS: Expression of torsin proteins in the central nervous system was analyzed by Western blot analysis and immunohistochemistry in human postmortem brain tissues. RESULTS: We generated polyclonal antipeptide antibodies directed against human torsin A and torsin B proteins. In Western blot analysis of normal human brain homogenates, the antibodies specifically recognized 38-kd endogenous torsin A and 62-kd endogenous torsin B. Absorption controls showed that labeling was blocked by cognate peptide used for immunization. Immunolocalization studies revealed that torsin A and torsin B were widely expressed throughout the human central nervous system. Both proteins displayed cytoplasmic distribution, although torsin B localization in some neurons was perinuclear. Strong labeling of neuronal processes was detected for both proteins. CONCLUSIONS: Torsin A and torsin B have similar distribution in the central nervous system, although their subcellular localization is not identical. Strong expression in neuronal processes points to a potential role for torsin proteins in synaptic functioning.
Asunto(s)
Encéfalo/metabolismo , Proteínas Portadoras/metabolismo , Chaperonas Moleculares , Neuronas/metabolismo , Animales , Anticuerpos/metabolismo , Especificidad de Anticuerpos , Humanos , ConejosRESUMEN
Neurofibromatosis type 1 (NF1) is a commonly inherited autosomal dominant disorder. Previous studies indicated that mice homozygous for a null mutation in Nf1 exhibit mid-gestation lethality, whereas heterozygous mice have an increased predisposition to tumors and learning impairments. Here we show that mice lacking the alternatively spliced exon 23a, which modifies the GTPase-activating protein (GAP) domain of Nf1, are viable and physically normal, and do not have an increased tumor predisposition, but show specific learning impairments. Our findings have implications for the development of a treatment for the learning disabilities associated with NF1 and indicate that the GAP domain of NF1 modulates learning and memory.
Asunto(s)
Exones , Predisposición Genética a la Enfermedad , Discapacidades para el Aprendizaje/genética , Neoplasias Experimentales/genética , Animales , Secuencia de Bases , Cartilla de ADN , Genes de Neurofibromatosis 1 , Ratones , Neurofibromatosis 1/genética , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Inactivating mutations of the gene encoding parkin are responsible for autosomal recessive juvenile parkinsonism (AR-JP). However, little information is known about the function and distribution of parkin. We generated antibodies to two different peptides of parkin. By Western blot analysis and immunohistochemistry, we found that parkin is a 50-kd protein that is expressed in neuronal processes and cytoplasm of selected neurons in the basal ganglia, midbrain, cerebellum, and cerebral cortex. Unlike ubiquitin and alpha-synuclein, parkin labeling was not found in Lewy bodies of four sporadic Parkinson disease brains. Parkin was colocalized with actin filaments but not with microtubules in COS1 kidney cells and nerve growth factor-induced PC12 neurons. These results point to the importance of the cytoskeleton and associated proteins in neurodegeneration.
Asunto(s)
Citoesqueleto de Actina/química , Citoesqueleto/química , Técnica del Anticuerpo Fluorescente/métodos , Ligasas/análisis , Neuronas/química , Humanos , Immunoblotting , Inmunohistoquímica , Ubiquitina-Proteína LigasasRESUMEN
Instability of CAG DNA trinucleotide repeats is the mutational mechanism for several neurodegenerative diseases resulting in the expansion of a polyglutamine (polyQ) tract. Proteins with long polyQ tracts have an increased tendency to aggregate, often as truncated fragments forming ubiquitinated intranuclear inclusion bodies. We examined whether similar features define spinocerebellar ataxia type 2 (SCA2) pathogenesis using cultured cells, human brains and transgenic mouse lines. In SCA2 brains, we found cytoplasmic, but not nuclear, microaggregates. Mice expressing ataxin-2 with Q58 showed progressive functional deficits accompanied by loss of the Purkinje cell dendritic arbor and finally loss of Purkinje cells. Despite similar functional deficits and anatomical changes observed in ataxin-1[Q80] transgenic lines, ataxin-2[Q58] remained cytoplasmic without detectable ubiquitination.
Asunto(s)
Encéfalo/metabolismo , Núcleo Celular/metabolismo , Cuerpos de Inclusión/metabolismo , Biosíntesis de Proteínas , Ataxias Espinocerebelosas/etiología , Animales , Ataxinas , Western Blotting , Calbindinas , Línea Celular , Cerebelo/metabolismo , Citoplasma/metabolismo , Prueba de Esfuerzo , Proteínas Fluorescentes Verdes , Humanos , Inmunohistoquímica , Proteínas Luminiscentes/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos DBA , Ratones Transgénicos , Modelos Biológicos , Modelos Genéticos , Mutación , Proteínas del Tejido Nervioso , Péptidos/genética , Péptidos/metabolismo , Condicionamiento Físico Animal , Proteínas/genética , Proteínas/fisiología , Células de Purkinje/metabolismo , ARN/metabolismo , Proteínas Recombinantes de Fusión/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteína G de Unión al Calcio S100/metabolismo , Ataxias Espinocerebelosas/metabolismo , Factores de Tiempo , Transgenes , Ubiquitinas/biosíntesisRESUMEN
The neurofibromatosis 2 tumor suppressor protein schwannomin/merlin is commonly mutated in schwannomas and meningiomas. Schwannomin, a member of the 4.1 family of proteins, which are known to link the cytoskeleton to the plasma membrane, has little known function other than its ability to suppress tumor growth. Using yeast two-hybrid interaction cloning, we identified the HGF-regulated tyrosine kinase substrate (HRS) as a schwannomin interactor. We verified the interaction by both immunoprecipitation of endogenous HRS with endogenous schwannomin in vivo as well as by using bacterially purified HRS and schwannomin in vitro. We narrowed the regions of interaction to include schwannomin residues 256-579 and HRS residues from 480 to the end of either of two HRS isoforms. Schwannomin molecules with a L46R, L360P, L535P or Q538P missense mutation demonstrated reduced affinity for HRS binding. As HRS is associated with early endosomes and may mediate receptor translocation to the lysosome, we demonstrated that schwannomin and HRS co-localize at endosomes using the early endosome antigen 1 in STS26T Schwann cells by indirect immunofluorescence. The identification of schwannomin as a HRS interactor implicates schwannomin in HRS-mediated cell signaling.
Asunto(s)
Proteínas de la Membrana/metabolismo , Fosfoproteínas/metabolismo , Adulto , Sitios de Unión , ADN Complementario/química , ADN Complementario/genética , Complejos de Clasificación Endosomal Requeridos para el Transporte , Endosomas/química , Genes de la Neurofibromatosis 2/genética , Humanos , Proteínas de la Membrana/genética , Microscopía Confocal , Microscopía Fluorescente , Datos de Secuencia Molecular , Mutación , Neurofibromina 2 , Fosfoproteínas/genética , Plásmidos , Unión Proteica , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Análisis de Secuencia de ADN , Células Tumorales CultivadasRESUMEN
Spinocerebellar ataxia type 2 (SCA2) is caused by expansion of a polyglutamine tract in ataxin-2, a protein of unknown function. Using the yeast two-hybrid system, we identified a novel protein, A2BP1 (ataxin-2 binding protein 1) which binds to the C-terminus of ataxin-2. Northern blot analysis showed that A2BP1 was predominantly expressed in muscle and brain. By immunocfluorescent staining, A2BP1 and ataxin-2 were both localized to the trans -Golgi network. Immunocytochemistry showed that A2BP1 was expressed in the cytoplasm of Purkinje cells and dentate neurons in a pattern similar to that seen for ataxin-2 labeling. Western blot analysis of subcellular fractions indicated enrichment of A2BP1 in the same fractions as ataxin-2. Sequence analysis of the A2BP1 cDNA revealed an RNP motif that is highly conserved among RNA-binding proteins. A2BP1 had striking homology with a human cDNA clone, P83A20, of unknown function and at least two copies of A2BP1 homologs are found in the Caenorhabditis elegans genome database. A2BP1 and related proteins appear to form a novel gene family sharing RNA-binding motifs.
Asunto(s)
Proteínas de Caenorhabditis elegans , Proteínas/metabolismo , Proteínas de Unión al ARN/genética , Proteínas de Unión al ARN/metabolismo , Adulto , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Ataxinas , Northern Blotting , Western Blotting , Encéfalo/metabolismo , ADN Complementario/metabolismo , Técnica del Anticuerpo Fluorescente , Humanos , Datos de Secuencia Molecular , Músculos/metabolismo , Proteínas del Tejido Nervioso , Neuronas/metabolismo , Pruebas de Precipitina , Unión Proteica , Proteínas/química , Células de Purkinje/metabolismo , Proteínas de Unión al ARN/biosíntesis , Homología de Secuencia de Aminoácido , Ataxias Espinocerebelosas/metabolismo , Distribución Tisular , Técnicas del Sistema de Dos HíbridosRESUMEN
Spinocerebellar ataxia type 2 (SCA2) is caused by expansion of a CAG trinucleotide repeat located in the coding region of the human SCA2 gene. The SCA2 gene product, ataxin-2, is a basic protein with two domains (Sm1 and Sm2) implicated in RNA splicing and protein interaction. However, the wild-type function of ataxin-2 is yet to be determined. To help clarify the function of ataxin-2, we produced antibodies to three antigenic peptides of ataxin-2 and analyzed the expression pattern of ataxin-2 in normal and SCA2 adult brains and cerebellum at different developmental stages. These studies revealed that (1) both wild-type and mutant forms of ataxin-2 were synthesized; (2) the wild-type ataxin-2 was localized in the cytoplasm in specific neuronal groups with strong labeling of Purkinje cells; (3) the level of ataxin-2 increased with age in Purkinje cells of normal individuals; and (4) ataxin-2-like immunoreactivity in SCA2 brain tissues was more intense than in normal brain tissues, and intranuclear ubiquitinated inclusions were not seen in SCA2 brain tissues.
Asunto(s)
Enfermedad de Alzheimer/metabolismo , Encéfalo/metabolismo , Proteínas/análisis , Degeneraciones Espinocerebelosas/metabolismo , Adulto , Anciano , Ataxinas , Femenino , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Proteínas del Tejido NerviosoRESUMEN
Spinocerebellar ataxia type 2 (SCA2) is caused by expansion of a CAG trinucleotide repeat located in the coding region of the human SCA2 gene. Sequence analysis revealed that SCA2 is a novel gene of unknown function. In order to provide insights into the molecular mechanisms of pathogenesis of SCA2 and to identify conserved domains, we isolated and characterized the mouse homolog of the SCA2 gene. Sequence and amino acid analysis revealed 89% identity at the nucleotide and 91% identity at the amino acid level. However, there was no extended polyglutamine tract in the mouse SCA2 cDNA, suggesting that the normal function of SCA2 is not dependent on this domain. Northern blot analysis of different mouse tissues indicated that the mouse SCA2 gene was expressed in most tissues, but at varying levels. Alternative splicing seen in human SCA2 was conserved in the mouse. By northern blot analysis, SCA2 was expressed during embryogenesis as early as day 8 of gestation (E8). Immunohistochemical staining using affinity-purified antibodies demonstrated that ataxin 2 was expressed in the cytoplasm of Purkinje cells as well as in other neurons of the CNS.
Asunto(s)
Empalme Alternativo , ADN Complementario/genética , Biosíntesis de Proteínas , Proteínas/genética , Degeneraciones Espinocerebelosas/genética , Secuencia de Aminoácidos , Animales , Ataxinas , Secuencia de Bases , Humanos , Ratones , Datos de Secuencia Molecular , Proteínas del Tejido Nervioso , Alineación de Secuencia , Análisis de Secuencia , Repeticiones de Trinucleótidos/genéticaRESUMEN
NF2 is the most commonly mutated gene in benign tumours of the human nervous system. The NF2 protein, called schwannomin or merlin, is absent in virtually all schwannomas, and many meningiomas and ependymomas. Using the yeast two-hybrid system, we identified betaII-spectrin (also known as fodrin) as a schwannomin-binding protein. Interaction occurred between the carboxy-terminal domain of schwannomin isoform 2 and the ankyrin-binding region of betaII-spectrin. Isoform 1 of schwannomin, in contrast, interacted weakly with betaII-spectrin, presumably because of its strong self-interaction. Thus, alternative splicing of NF2 may regulate betaII-spectrin binding. Schwannomin co-immunoprecipitated with betaII-spectrin at physiological concentrations. The two proteins interacted in vitro and co-localized in several target tissues and in STS26T cells. Three naturally occurring NF2 missense mutations showed reduced, but not absent, betaII-spectrin binding, suggesting an explanation for the milder phenotypes seen in patients with missense mutations. STS26T cells treated with NF2 antisense oligonucleotides showed alterations of the actin cytoskeleton. Schwannomin itself lacks the actin binding sites found in ezrin, radixin and moesin, suggesting that signalling to the actin cytoskeleton occurs via actin-binding sites on betaII-spectrin. Thus, schwannomin is a tumour suppressor directly involved in actin-cytoskeleton organization, which suggests that alterations in the cytoskeleton are an early event in the pathogenesis of some tumour types.
Asunto(s)
Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Espectrina/metabolismo , Actinas/análisis , Actinas/efectos de los fármacos , Animales , Ancirinas/metabolismo , Sitios de Unión , Cricetinae , Citoesqueleto/química , Citoesqueleto/efectos de los fármacos , Genes de la Neurofibromatosis 2/genética , Humanos , Inmunohistoquímica , Proteínas de la Membrana/química , Proteínas de Neoplasias/metabolismo , Neurofibromina 2 , Oligonucleótidos Antisentido/genética , Oligonucleótidos Antisentido/farmacología , Pruebas de Precipitina , Unión Proteica , Espectrina/química , Distribución Tisular , Células Tumorales CultivadasRESUMEN
The expression patterns of presenilin 1 (PS1) and presenilin 2 (PS2) in human normal and Alzheimer disease (AD) brains were investigated using antibodies to specific N-terminal peptides of PS1 (Alzh14A and Alzh14B) and PS2 (Alzh1A-AB). The antibodies to peptides Alzh14A (Alzh14A-AB) and Alzh14B (Alzh14B-AB) detected the full-length protein (approximately 63 kDa) and the N-terminal-processed fragment (36 kDa) of PS1, while the Alzh1A-AB detected mainly the N-terminal-processed fragment (36 kDa) of PS2. Immunofluorescent staining detected by confocal microscopy suggested that both native PS1 and PS2 are localized mainly in the Golgi/ER apparatus. Immunohistochemical studies of human temporal lobes from 2 normal and 5 sporadic Alzheimer brains revealed high levels of PS1 and PS2 expression in the granule cell layer and pyramidal neurons of the hippocampus. Strong immunoreactivity was found in reactive astrocytes and neurofibrillary tangles of all 5 Alzheimer brains. In contrast, only 2 sporadic Alzheimer brains showed presenilin-positive neuritic plaques. These observations suggest that presenilins may be involved in the pathology of some cases of sporadic AD.
Asunto(s)
Enfermedad de Alzheimer/metabolismo , Encéfalo/metabolismo , Membranas Intracelulares/metabolismo , Proteínas de la Membrana/metabolismo , Neuronas/metabolismo , Enfermedad de Alzheimer/patología , Animales , Encéfalo/patología , Hipocampo/metabolismo , Hipocampo/patología , Humanos , Inmunohistoquímica , Ratones , Presenilina-1 , Presenilina-2 , Valores de Referencia , Distribución Tisular , Células Tumorales CultivadasRESUMEN
In addition to schwannomas, patients with neurofibromatosis type 2 (NF2) frequently develop meningiomas and occasionally, ependymomas. Using DNA and protein analyses, we have shown NF2 gene mutations and lack of the gene product schwannomin in 29 schwannomas, 10 meningiomas, and in 7 ependymomas. We have raised antibodies (ABs) to peptides from the C-terminal (5990-AB) and N-terminal (5991-AB) domains of schwannomin. The ABs specifically detected a 65 kDa protein in a Schwann cell line and recognized schwannomin in the cytoplasm of Schwann cells (SCH), perineurial cells, and vestibular ganglion neurons. None of the 29 schwannomas were stained by the 5990-AB. Only 4 schwannomas were stained by the 5991-AB, indicating that most truncated schwannomins were unstable or not expressed in schwannomas. Seven of 10 meningiomas, including 3 tumors from NF2 patients, were not stained by either 5990-AB or 5991-AB. Only 2 of 7 ependymomas lacked schwannomin. Complete lack of schwannomin in these tumors supports a tumor suppressor function for schwannomin in some meningiomas and ependymomas. All tumors showed staining with an antibody to a C-terminal peptide of neurofibromin, confirming that full-length neurofibromin is present in these vestibular schwannomas, meningiomas, and ependymomas. The presence of schwannomin in some meningiomas and in the majority of ependymomas indicates that additional genes are likely to play a role in tumorigenesis of these tumors.
Asunto(s)
Neoplasias Encefálicas/metabolismo , Ependimoma/metabolismo , Proteínas de la Membrana/metabolismo , Neoplasias Meníngeas/metabolismo , Meningioma/metabolismo , Neurilemoma/metabolismo , Proteínas/metabolismo , Enfermedades Vestibulares/metabolismo , Humanos , Inmunohistoquímica , Proteínas de la Membrana/genética , Mutación , Neurofibromatosis 2/genética , Neurofibromina 1 , Neurofibromina 2RESUMEN
The expression pattern of presenilin 1 (PS-1) in adult mouse brain was investigated using antibodies to specific peptides of PS-1. One antibody, Alz14A, specifically detected a 53 kDa protein in retinoic acid-treated P19 cells and mouse brain protein extracts consistent with the predicted PS-1 molecular weight. Immunohistochemical staining revealed that PS-1 was localized predominantly in large neurons in areas that were known to be affected by Alzheimer disease (AD) such as the hippocampal formation, entorhinal cortex and the subiculum. Selected neurons in other regions not known to be directly affected by AD, such as thalamic nuclei, Purkinje cells, large neurons in the brainstem and the gray matter of the spinal cord, and the dorsal root ganglion, also expressed PS-1. These observations suggest that other as yet identified factors might interact with mutated presenilins to cause neurodegeneration in AD-affected areas.
Asunto(s)
Encéfalo/metabolismo , Proteínas de la Membrana/metabolismo , Animales , Células Cultivadas/metabolismo , Inmunohistoquímica , Ratones , Ratones Endogámicos , Presenilina-1RESUMEN
Neurofibromatosis 2 (NF2) is an autosomal dominant inherited disorder that predisposes to benign tumors of the nervous system as well as a variety of ocular abnormalities. In contrast to NF1, NF2 is associated with only minor developmental abnormalities. The human NF2 gene encodes a tumor suppressor protein, termed schwannomin or merlin, which is a member of a superfamily of proteins thought to link cytoskeletal elements to cell membrane components. To determine the pattern of NF2 gene expression in mouse embryos, we sequenced the mouse NF2 gene and used in situ hybridization and antischwannomin antibodies to determine the developmental expression of the NF2 gene. Schwannomin was detected in most differentiated tissues but was undetectable in undifferentiated tissues. In particular, schwannomin was not detectable in mitotic neuroepithelial cells, the perichondrium, the liver, the neocortex, and the ventricular zone of the developing cerebral cortex. In the heart, expression was observed in all developmental stages beginning on embryonic day 8. In the eye, which shows developmental abnormalities in NF2 patients, expression was detected in the cells of the lens and in the pigment epithelium but weakly detected in retinal neurons. The most striking example of tightly regulated NF2 expression was observed in cells migrating from the ventricular zone to the cortical plate on embryonic days 15 and 16. Only cells in the intermediate zone expressed schwannomin, indicating that schwannomin may play an important role in cellular migration.
Asunto(s)
Desarrollo Embrionario y Fetal/genética , Regulación del Desarrollo de la Expresión Génica/fisiología , Proteínas de la Membrana/genética , Neurofibromatosis 2/genética , Secuencia de Aminoácidos , Animales , Especificidad de Anticuerpos , Embrión de Mamíferos/química , Genes de la Neurofibromatosis 2/genética , Proteínas de la Membrana/análisis , Ratones , Datos de Secuencia Molecular , Sistema Nervioso/química , Sistema Nervioso/embriología , Neurofibromina 2 , Especificidad de Órganos , ARN Mensajero/análisis , ARN Neoplásico/análisisRESUMEN
Mutations in the neurofibromatosis 2 (NF2) gene are the predominant cause in the development of sporadic schwannomas and are also involved in the pathogenesis of meningiomas and ependymomas. The product of the NF2 gene, termed merlin or schwannomin, is thought to act as a tumor suppressor protein. Although its protein sequence shows homology to proteins that are known to link the cytoskeleton to the cell membrane, no direct evidence for this function has been obtained. We used antisense phosphorothioate oligodeoxynucleotides (pODNs) complementary to the human NF2 cDNA sequence and transfected them into Schwann-like STS26T cells permeabilized by streptolysin 0. Changes in cell morphology and attachment were observed at 12 to 24 h and continued up to 48 h post transfection. Cells were rounded and easily dislodged from the substratum at 12-24 h. These changes were reversible and cells became bipolar with thin protrusions and began to reattach to the substratum after 48 h. Normal morphology and adhesion were observed at 72 h post transfection. Morphological changes were due to suppression of schwannomin synthesis. Immunoprecipitations with antischwannomin antibodies showed schwannomin to be almost absent 3 h after treatment with antisense pODNs and to be significantly suppressed up to 12 h post transfection whereas beta-actin levels remained unchanged. The morphological changes were not the result of cell death, but resulted in increased cell proliferation. These data demonstrate that antisense oligonucleotides can be successfully employed to suppress schwannomin synthesis and indicate that schwannomin may belong to a class of tumor suppressor genes that provide a link between cell adhesion and tumorigenesis.
Asunto(s)
Genes de la Neurofibromatosis 2 , Proteínas de la Membrana/fisiología , Proteínas de Neoplasias/fisiología , Oligonucleótidos Antisentido/farmacología , Secuencia de Aminoácidos , Secuencia de Bases , Adhesión Celular/efectos de los fármacos , Tamaño de la Célula/efectos de los fármacos , Citoesqueleto/ultraestructura , ADN Complementario/genética , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Humanos , Proteínas de la Membrana/biosíntesis , Proteínas de la Membrana/genética , Datos de Secuencia Molecular , Proteínas de Neoplasias/biosíntesis , Proteínas de Neoplasias/genética , Neurilemoma/patología , Neurofibromina 2 , Tionucleótidos , Transfección , Células Tumorales Cultivadas/ultraestructuraRESUMEN
Mutations in the neurofibromatosis type 2 (NF2) gene predispose individuals to the development of nervous system tumors and ocular abnormalities. The NF2 gene product, schwannomin, is a member of a superfamily of proteins thought to link cytoskeletal elements to cell membrane components. These proteins share significant homologies in the N-terminal and alpha-helical domains, but diverge in the C-terminus. During our efforts to characterize mouse NF2 transcripts, we identified four different transcripts by cDNA analysis and reverse-transcribed PCR that contained different sequences in the 3' end of the coding sequences. In human cell lines three isoforms encoding two distinct schwannomins were detected. The mouse and human transcripts containing 61 and 60 bp inserts, respectively, have not been previously described. The isoforms encode schwannomins with significantly altered C-termini and were expressed at different relative levels in adult mouse tissues and during mouse embryogenesis. These results suggest that schwannomin isoforms have distinct functional roles and predict the existence of human mutations involving the C-terminus of schwannomin.
Asunto(s)
Genes de la Neurofibromatosis 2 , Proteínas de la Membrana/genética , Ratones/genética , Neurofibromatosis 2/genética , ARN Mensajero/genética , Transcripción Genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Línea Celular , Humanos , Proteínas de la Membrana/química , Ratones/embriología , Ratones/crecimiento & desarrollo , Datos de Secuencia Molecular , Neurofibromina 2 , Reacción en Cadena de la Polimerasa , ARN Neoplásico/genéticaRESUMEN
Schwannomas are common tumors of the nervous system and are frequently found in patients with neurofibromatosis (NF) 2. Although loss of heterozygosity in NF2 tumors suggests that the NF2 gene functions as a tumor suppressor gene, the NF2 gene shows amino acid sequence homology to structural proteins in one of which dominantly acting mutations have been described. We performed a mutational analysis in 30 vestibular schwannomas and examined the effect of mutations on the NF2 protein. We detected 18 mutations in 30 vestibular schwannomas of which seven contained loss or mutation of both NF2 alleles. Most mutations were predicted to result in a truncated protein. Mutational hot spots were not identified. Immunocytochemical studies using antibodies to the NF2 protein showed complete absence of staining in tumor Schwann cells, whereas staining was observed in normal vestibular nerve. These data indicate that loss of NF2 protein function is a necessary step in schwannoma pathogenesis and that the NF2 gene functions as a recessive tumor suppressor gene.
Asunto(s)
Genes de la Neurofibromatosis 2 , Mutación , Neurofibromatosis 2/genética , Secuencia de Aminoácidos , Secuencia de Bases , Análisis Mutacional de ADN , Cartilla de ADN , Exones , Mutación del Sistema de Lectura , Marcadores Genéticos , Humanos , Inmunohistoquímica , Proteínas de la Membrana/genética , Datos de Secuencia Molecular , Proteínas de Neoplasias/genética , Neurofibromatosis 2/patología , Neurofibromina 2 , Mutación Puntual , Reacción en Cadena de la Polimerasa , Valores de Referencia , Eliminación de Secuencia , Homología de Secuencia de Aminoácido , Nervio Vestibular/citología , Nervio Vestibular/metabolismoRESUMEN
Mutations in the NF1 gene may cause developmental abnormalities and the formation of a variety of tumors of neural crest origin in humans. The NF1 gene codes for a large protein, neurofibromin (nf), which is structurally and functionally related to yeast and human ras-GTPase-activating proteins (ras-GAPs). Recently, two transcripts coding for type I and type II nf with different ras-GAP activity have been identified. Since ras proteins do not appear to be significantly regulated during mouse development, we examined if differential expression of neurofibromins may provide evidence for a role of nfs in regulating ras-mediated cell proliferation and differentiation. Nfs were expressed as early as E8. At E11 a marked increase of NF1 transcripts occurred and was associated with expression of nfs in all tissues. Type I and type II nfs each showed a different time course of expression and tissue localization, with type II nf present mainly from E8 through E10, although in the heart type II nf was present at E12. In some tissues such as heart and dorsal root ganglia rapid increases and decreases of nfs were detected related to differentiation of these tissues. These results are consistent with a role of nfs in regulating ras-mediated cell proliferation and differentiation during development and support distinct functional roles for type I and type II nfs.