ASSESSMENT OF THE LIPID PEROXIDE OXIDATION PROCESSES AND ANTIOXIDANT PROTECTION IN THE TREATMENT OF RATS THERMAL BURNING BY DOXICYCLINE AS A SYNTHETIC INHIBITOR OF MATRIX METALLOPROTEINASES IN EXPERIMENT.

Aim - to assess the effects of doxycycline on the state of lipid peroxidation processes and the activity of the antioxidant system. The study was performed on 144 rats of the WAG population weighing 200-250 g (6 rats in each group). Animals with thermal burns were injected with the test drug doxycycline, as well as reference drugs - thiotriazolin and methyluracil orally in starch suspensionafter thermal exposure and daily during the entire experiment period (28 days). Animals were removed from the experiment in accordance with the rules of bioethics on the 7th, 14th, 21st and 28th day. As a result of the research, the most intense influence of doxycycline on the processes of lipid peroxidation in the blood serum of rats was found - a decrease in DC activity by the end of the experiment (regardless of dose) and TBA-AP starting from the 14th day of observation reaching the value of intact rats (at a dose of 30 mg/kg). At the same time, an increase in the activity of enzymes of the antioxidant system was noted - the level of catalase from the 14th day (significantly higher than the control group regardless of dose) and the level of ceruloplasmin (from the second week of observation the indicator reached intact values ​​at a dose of 30 mg/kg). Reference drugs were inferior to doxycycline in their effectiveness. The data obtained may indicate the possibility of reducing the healing time of a thermal burn due to the suppression of excessive free radical oxidation activity and activation of antioxidant protection.

DYNAMICS OF INDICATORS OF ANTIOXIDANT PROTECTION IN RESPONSE TO THE APPLICATION OF SODIUM POLY-(2.5- DIHYDROXYPHENILEN)-4- THIOSULFATE ACID IN EXPERIMENTAL ACUTE KIDNEY INJURY.

The purpose of the study is to establish the effect of the investigated drug PDT-Na on the parameters of free radical lipid oxidation and the system of antioxidant defence of the kidneys in rats on models of acute renal failure and gentamicin nephropathy. The studies were conducted on 96 white mature, full-grown non-linear rats of both sexes weighing 150-170 grams. The animals were divided into 16 groups (5 groups per model and one intact group): the animals of the experimental, reference and control pathology groups (1) received the corresponding nephrotoxin. In each model the groups of rats received the following treatment: experimental (2) - PDT-Na, reference - mexidol (3), hofitol - (4) and thiotriazolin (5). The results of the study show that under the conditions of acute kidney injury the PDT-Na assists to reduce the primary intermediates - diene conjugates and secondary intermediates of lipid peroxidation - TBA-reactive products, activation of enzymes of catalase antioxidant defence and superoxide dismutase and accumulation to the almost intact level of GSH endogenous antioxidant.The abovementioned information indicates that the drug of the pharmacological group - antihypoxicant PDT-Na with previously established nephroprotective activity has shown antioxidant, antiradical and cytoprotective properties during the experiment. This is a promising drug for complex treatment of kidney diseases.