Changes in the hydrocarbon-synthesizing activity during growth of Botryococcus braunii B70.

Botryococcus braunii is a green, colonial microalga that produces large amounts of hydrocarbons. B. braunii B70 was estimated to be B race by the incorporation of radioactivity from l-[methyl(14)C]-methionine into hydrocarbon. The hydrocarbon-synthesizing activity of B70 cells was determined by feeding experiments using (14)C-compounds. NaH(14)CO(3) incorporation rate into the hydrocarbon was high in the early logarithmic growth phase but it declined thereafter. Hydrocarbon-synthesizing activity from [2-(14)C] pyruvate in 15-day cells was 80% of that in 5-day cells. In contrast, hydrocarbon-synthesizing activity from NaH(14)CO(3) and l-[methyl(14)C]-methionine decreased remarkably by 15 days after inoculation. Hence, the allocation of carbon was a regulatory step in hydrocarbon biosynthesis during the early logarithmic growth phase. The high activity of pentose phosphate pathway in the early logarithmic growth was seemed to be the contribution of the supply of NADPH for botryococcene synthesis.

[Introduction patient analysis from the medical institution in Shinjuku].

The number of introduction patients from July 22, 2003 to the end of December was 10,329 persons. The introduction from the medical institution in Shinjuku was 2,175 persons (21.1%). We investigated 2,175 persons for man and woman, age, the department of medical examination, the address ground, etc. Shinjuku-ku is special area; there is much population of daytime. We analyzed the present condition and analyzed needs this time.