Novel thiol- amine- and amino acid functional xylan derivatives synthesized by thiol-ene reaction.

In the present work, novel thioether xylans were synthesized via a simple procedure using water as solvent. First, allyl groups were introduced on the backbone of xylan by etherification of allyl chloride in aqueous alkaline conditions at 40°C, providing degree of substitution (DS) values up to 0.49. On the second step, the allyl groups were reacted with thioacetic acid, cysteamine hydrochloride or cysteine providing novel thiol-, amine- or amino acid functionalized xylans. The presented modular approach offers broad possibilities for developing new polysaccharide based materials. The thioacetic acid - ene reaction is reported for the first time for polysaccharide modification, yielding a protected thiol that can be stored at atmospheric conditions and can be deprotected by simple hydrolysis just prior to use, providing a versatile water soluble polythiol. The free thiol-groups were utilized for hydrogel formation through thiol-thiol oxidative coupling, allowing good control over the hydrogel shape, such as 3D hydrogel scaffolds and cross-linked foams. Further, the thiol-containing xylan was used to modify filter paper surface by a simple dipping method, which provides a novel and convenient way for introducing thiol-functionality on paper surface.

Continuous propionic acid production with Propionibacterium acidipropionici immobilized in a novel xylan hydrogel matrix.

The cell immobilization potential of a novel xylan based disulfide-crosslinked hydrogel matrix reinforced with cellulose nanocrystals was studied with continuous cultivation of Propionibacterium acidipropionici using various dilution rates. The cells were immobilized to hydrogel beads suspended freely in the fermentation broth or else packed into a column connected to a stirred tank reactor. The maximum propionic acid productivity for the combined stirred tank and column was 0.88gL(-1)h(-1) and the maximum productivity for the column was determined to be 1.39gL(-1)h(-1). The maximum propionic acid titer for the combined system was 13.9gL(-1) with a dilution rate of 0.06h(-1). Dry cell density of 99.7gL(-1) was obtained within the column packed with hydrogel beads and productivity of 1.02gL(-1)h(-1) was maintained in the column even with the high circulation rate of 3.37h(-1).

Climatic control on plant and soil δ(13)C along an altitudinal transect of Lushan mountain in subtropical china: characteristics and interpretation of soil carbon dynamics.

Decreasing temperature and increasing precipitation along altitude gradients are typical mountain climate in subtropical China. In such a climate regime, identifying the patterns of the C stable isotope composition (δ(13)C) in plants and soils and their relations to the context of climate change is essential. In this study, the patterns of δ(13)C variation were investigated for tree leaves, litters, and soils in the natural secondary forests at four altitudes (219, 405, 780, and 1268 m a.s.l.) in Lushan Mountain, central subtropical China. For the dominant trees, both leaf and leaf-litter δ(13)C decreased as altitude increased from low to high altitude, whereas surface soil δ(13)C increased. The lower leaf δ(13)C at high altitudes was associated with the high moisture-related discrimination, while the high soil δ(13)C is attributed to the low temperature-induced decay. At each altitude, soil δ(13)C became enriched with soil depth. Soil δ(13)C increased with soil C concentrations and altitude, but decreased with soil depth. A negative relationship was also found between O-alkyl C and δ(13)C in litter and soil, whereas a positive relationship was observed between aromatic C and δ(13)C. Lower temperature and higher moisture at high altitudes are the predominant control factors of δ(13)C variation in plants and soils. These results help understand C dynamics in the context of global warming.

The effects of soil and air temperature on CO2 exchange and net biomass accumulation in Norway spruce, Scots pine and silver birch seedlings.

Soil temperature is proposed to affect the photosynthetic rate and carbon allocation in boreal trees through sink limitation. The aim of this study was to investigate the effect of temperature on CO(2) exchange, biomass partitioning and ectomycorrhizal (ECM) fungi of boreal tree species. We measured carbon allocation, above- and below-ground CO(2) exchange and the species composition of associated ECM fungi in the rhizosphere of Scots pine (Pinus sylvestris L.), Norway spruce (Picea abies K.) and silver birch (Betula pendula Roth) seedlings grown in soil maintained at 7-12, 12-15 and 16-22 °C. We found increased root biomass and photosynthetic rate at higher soil temperatures, but simultaneously with photosynthesis rate, higher temperature generally increased soil respiration as well as shoot, and root and rhizosphere respiration. The net CO(2) exchange and seedling biomass did not increase significantly with increasing temperature due to a concomitant increase in carbon assimilation and respiration rates. The 2-month-long growth period in different soil temperatures did not alter the ECM fungi species composition and the below-ground carbon sink strength did not seem to be directly related to ECM biomass and species composition in any of the tree species. Ectomycorrhizal species composition and number of mycorrhiza did not explain the CO(2) exchange results at different temperatures.

Archaeal communities in boreal forest tree rhizospheres respond to changing soil temperatures.

Temperature has generally great effects on both the activity and composition of microbial communities in different soils. We tested the impact of soil temperature and three different boreal forest tree species on the archaeal populations in the bulk soil, rhizosphere, and mycorrhizosphere. Scots pine, silver birch, and Norway spruce seedlings were grown in forest humus microcosms at three different temperatures, 7-11.5°C (night-day temperature), 12-16°C, and 16-22°C, of which 12-16°C represents the typical mid-summer soil temperature in Finnish forests. RNA and DNA were extracted from indigenous ectomycorrhiza, non-mycorrhizal long roots, and boreal forest humus and tested for the presence of archaea by nested PCR of the archaeal 16S rRNA gene followed by denaturing gradient gel electrophoresis (DGGE) profiling and sequencing. Methanogenic Euryarchaeota belonging to Methanolobus sp. and Methanosaeta sp. were detected on the roots and mycorrhiza. The most commonly detected archaeal 16S rRNA gene sequences belonged to group I.1c Crenarchaeota, which are typically found in boreal and alpine forest soils. Interestingly, also one sequence belonging to group I.1b Crenarchaeota was detected from Scots pine mycorrhiza although sequences of this group are usually found in agricultural and forest soils in temperate areas. Tree- and temperature-related shifts in the archaeal population structure were observed. A clear decrease in crenarchaeotal DGGE band number was seen with increasing temperature, and correspondingly, the number of euryarchaeotal DGGE bands, mostly methanogens, increased. The greatest diversity of archaeal DGGE bands was detected in Scots pine roots and mycorrhizas. No archaea were detected from humus samples from microcosms without tree seedling, indicating that the archaea found in the mycorrhizosphere and root systems were dependent on the plant host. The detection of archaeal 16S rRNA gene sequences from both RNA and DNA extractions show that the archaeal populations were living and that they may have significant contribution to the methane cycle in boreal forest soil, especially when soil temperatures rise.

Effects of clear-cutting and soil preparation on natural 15N abundance in the soil and needles of two boreal conifer tree species.

This study presents the impacts of clear-cutting and site preparation on soil and needle 15N-fractionation of Scots pine (Pinus sylvestris, L.) and Norway spruce (Picea abies (L.), Karst). Three microsites on different methods of site preparation were used: (i) mound (broken O/E/B horizons piled upside down over undisturbed humus), (ii) deep (exposed C-horizon) and (iii) shallow (exposed E/B horizon). We found significant differences between species, between closed forest and clear-cuts as well as between different site preparations. For instance, in the context of interspecific variations, the mean needle nitrogen concentrations of both seedlings (1.15,+/-0.10 %) and mature (1.09,+/-0.07 %) pine trees were significantly higher compared to corresponding needle concentrations of seedlings (0.88,+/-0.06 %) and mature trees (0.79,+/-0.02 %) of spruce. Similarly, we observed significantly more 15N-enriched needles of mature spruces (-4.0,+/-0.20 per thousand) as well as of seedlings (-5.0,+/-0.11 per thousand) relative to that of mature pine needles (-5.6,+/-0.10 per thousand) and seedlings (-6.0,+/-0.31 per thousand). These variations were assumed to be caused by the variation in mycorrhizal associations between the species. We assume that the proportion of mycorrhizal N-uptake of pines might have been larger than that of spruce. Regarding the clear-cut effects on N and 15N of both tree species, we observed that, in the mature natural stand, needle N concentrations of both pine (1.09,+/-0.07 %) and spruce (0.79,+/-0.02 %) tree species did not change significantly after clear-cutting (pine: 1.01,+/-0.06 %; spruce: 0.74,+/-0.04 % ). However, clear-cutting resulted in the significant increase in needle 15N natural abundance of both pine (-2.70,+/-0.06 per thousand) and spruce (-2.09,+/-0.05 per thousand) in comparison to that of natural stand (pine:-5.60,+/-0.10 per thousand; spruce:-4.00,+/-0.20 per thousand), which is assumed to be due to the increased level of nitrification and leaching of nitrate after clear-cutting. In the context of site preparation methods, soil and needle N were observed to be more 15N-enriched in deep and shallow treatment sites compared to that of closed forest site and untreated clear-cut site, which indicated that the main source of N uptake seems to be mainly directly from the soil of the corresponding horizons of mineral soil with higher delta15N.

An estimate on the rainout of atmospheric CO2.

The CO2 in the atmosphere is in contact with water vapor and rain droplets forming CO2 x H2O, HCO3- and CO3(2-) . Global precipitation is about 505 x 1015 kg/a. Based on theoretical calculation for unpolluted air and measurement observations, we estimated that 100-270 x 10(12) gC/a are scavenged from the air by global precipitation. This roughly equals carbon emissions from volcanic sources or 2-6 per cent of current CO2 emissions. An inventory-based estimate on carbon removal in northwestern Europe supports the above calculation on global scale. With increasing CO2 concentration in the air, precipitation scavenging may increase.