Gastrointestinal and pulmonary parasites of working horses from Colombia.

Working equids are used in different countries for numerous purposes and regularly are crucial for multiple communities' income and profit. Historically, in Bogotá D.C. they were used as animal-powered vehicles but in 2013 it was initiated a substitution and adoption program as a result of society pressure and lawful processes. Infectious diseases and mainly parasitic diseases, poses a threat in the health and productivity of these animals. Our aim was to identify, by coproparasitological methods, the gastrointestinal and pulmonary parasite species infecting working horses submitted to the mentioned substitution and adoption program. Between May and December 2013 and February and July 2014, fresh faecal samples were obtained from 1004 and 648 horses from Bogotá D.C. and other Colombian municipalities, respectively. They were processed by flotation and Baermann tests in order to visualize faecal parasitic forms (eggs, oocysts, and larvae). Prevalences were calculated for each gastrointestinal parasite at origin and one year after, at destination, and analysed by age group and coinfection. At origin (Bogotá D.C.), prevalence for at least one parasite species was 87.5% and one year later, at destination (other municipalities), was 89.5%. The most prevalent species were strongyles (86.4-89.4%) followed by Parascaris spp. (0.7-6.2%), cestodes (Anoplocephalidae) (3.7-4.9%) and Oxyuris sp. (2.8-4.3%). Other species detected were Eimeria sp., Strongyloides sp. and Dictyocaulus sp. Coinfection by two or more species ranged between 14.4 and 38.3% being strongyle, Parascaris spp., Oxyuris sp. and cestodes (Anoplocephalidae) the most common species involved. Some parasitic infections commonly associated with younger animals (e.g. Parascaris spp.) were detected in all age groups. Flow patterns of parasites linking Bogotá D.C. and other municipalities are presented. Finally, these results support the widespread distribution of most of species and the plausible health and welfare impact of this infections in working equids submitted to particular epidemiological risks.

Epidemiology of Spotted Fever Group Rickettsioses and Acute Undifferentiated Febrile Illness in Villeta, Colombia.

Etiology of acute undifferentiated febrile syndrome (AUFS) is often unknown, leading to inaccurate diagnosis and treatment. Villeta town has been identified as an endemic area for spotted fever group (SFG) rickettsioses but little is known about possible amplifier hosts and other Rickettsia species different from Rickettsia rickettsii. Besides, few studies have approached other AUFS etiologies in the region. We investigated the role of dengue, leptospirosis, rickettsioses, human anaplasmosis, and Q fever as possible causes of AUFS in patients from Villeta. Sera specimens and ticks from animals as well as ticks from vegetation were studied for the presence of different Rickettsia spp. Among 104 sera from patients with AUFS, 16.4%, 24.0%, and 2.9% patients seroconverted to dengue, Leptospira, and SFG Rickettsia, respectively, with a case of probable coinfection or cross-reaction with Anaplasma phagocytophilum. None of the samples were reactive for Coxiella burnetii. Sera samples from 74 horses, 118 dogs, and 62 bovines were collected and showed 33.8%, 14.4%, and 50.0% of seroprevalence for SFG Rickettsia, respectively. A total of 1,287 ixodid ticks were collected from animals/vegetation and processed in pools for polymerase chain reaction. Among them, 1.7% was positive for Rickettsia genes, and Rickettsia amblyommii, R. rickettsii, and Rickettsia spp. were found. These results confirm the circulation of dengue, different SFG Rickettsia species and the relevance of other etiologies like leptospirosis and human anaplasmosis. Further studies must identify different epidemiological variables to establish proper surveillance and control programs.