Comparative Evaluation of Intrapulpal Thermal Changes during the Polymerization of Different Adhesive Resin Materials: An In Vitro Study.

AIM: Aim of this study was to assess the thermal changes within the pulp at the time of polymerization of three different adhesive resin materials. MATERIALS AND METHODS: Sixty human premolar teeth that had been recently extracted for orthodontic reasons and were devoid of dental caries/flaws were included in this research. Following preparation of the cavities, all 60 samples consisting of 20 premolars in every group, depending on the adhesive resins that were positioned as were assigned at random to one of the following three groups: Group I: RelyX self-adhesive resin cement; Group II: Breeze self-adhesive resin cement; Group III: Pan F self-etch adhesive resin cement. The temperature changes were calculated using a thermocouple wire attached to a digital thermometer. The dissimilarities amid the baseline temperature as well as the temperatures at different time intervals (1, 5, 10, and 15 minutes) were established. RESULTS: The thermal value was higher at first minute (1.84 ± 0.34) and gradually reduced at 5 minutes (1.36 ± 0.29), 10 minutes (0.62 ± 0.11), and 15 minutes (0.06 ± 0.03) in RelyX self-adhesive resin cement. The maximum thermal value was found at the first minute (2.66 ± 0.21) and gradually reduced at 5 minutes (1.42 ± 0.13), 10 minutes (0.86 ± 0.09), and 15 minutes (0.28 ± 0.01) in Breeze self-adhesive resin cement. The higher thermal value was found at the first minute (1.98 ± 0.19) and gradually reduced at 5 minutes (1.49 ± 0.14), 10 minutes (0.76 ± 0.10), and 15 minutes (0.16 ± 0.09) in Pan F self-etch adhesive resin cement and there was a statistically significant difference found between various time points and with all three adhesive resin material groups (p <0.001). CONCLUSION: This current research arrived at a conclusion that each of the three adhesive resin substances showed a safe temperature change within the pulp. However, the lowest heat scores within the pulp were depicted by RelyX self-adhesive resin in pursuit by Pan F self-etch adhesive resin cement as well as the Breeze self-adhesive resin cement in that order. CLINICAL SIGNIFICANCE: The clinical triumph of a restoration is dependent partially on the method of cementation, which is utilized to establish a connection amid the restoration and the tooth. Temporary and permanent pulp inflammation can be avoided by the decreased temperature changes in the adhesive resin cement at the time of polymerization.

Evaluation of cytotoxic effects of fungal origin nanosilver particles on oral cancer cell lines: An in vitro study.

Background: Oral cancer is often associated with poor prognosis and it is found that conventional treatment options cause severe side effects, adjacent tissue disfigurement, and loss of function. Recently, silver nanoparticles (AgNPs) paved their path for cancer treatment. Aim: This study aimed to investigate cytotoxic effects of fungal procured AgNPs on oral squamous cell carcinoma (SCC-9) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methodology: The silver nanoparticles were biosynthesized using the fungi Fusarium semitectum. Cell lines were cultured in a 1:1 ratio of Dulbecco's Modified Eagle's medium and Ham's F12 medium and subcultured in a T-75 cm2 flask. Cell count was adjusted to 1 × 105 cells/ml; 50,000 cells/well were seeded into a 96 well plate and incubated at 37°C, for 24 h in 5% CO2 humidified conditions. AgNPs (1.75-50 µl/ml) were added to the plates and further incubated at 37°C for another 24 h. Medium containing cells without AgNPs were used as a control group. Later, 20 µl of MTT was added to each well and incubated for 6 h at above-mentioned conditions. About 0.1 mL of Dulbecco's Modified Eagle's Medium solution was added to each well to solubilize formazan. The absorbance was measured using a Tecan reader at 540 nm. The experiment was repeated thrice independently. The percentage (%) inhibition of growth and the AgNP's concentration that prevents the cell growth by 50% (IC50) were determined. Results: Significant dose-dependent inhibition of the growth of SCC-9 cell lines was seen and IC50 was found at 12 µl/ml concentration of AgNPs. Conclusion: Biosynthesized AgNPs of fungal origin exhibit effective anticancer properties against the SCC-9 cell line.