Water pollution threats in important bird and biodiversity areas from Spain.

Chemical pollution is still an underestimated threat to surface waters from natural areas. This study has analysed the presence and distribution of 59 organic micropollutants (OMPs) including pharmaceuticals, lifestyle compounds, pesticides, organophosphate esters (OPEs), benzophenone and perfluoroalkyl substances (PFASs) in 411 water samples from 140 Important Bird and Biodiversity Areas (IBAs) from Spain, to evaluate the impact of these pollutants in sites of environmental relevance. Lifestyle compounds, pharmaceuticals and OPEs were the most ubiquitous chemical families, while pesticides and PFASs showed a detection frequency below 25% of the samples. The mean concentrations detected ranged from 0.1 to 301 ng/L. According to spatial data, agricultural surface has been identified as the most important source of all OMPs in natural areas. Lifestyle compounds and PFASs have been related to the presence of artificial surface and wastewater treatment plants (WWTPs) discharges, which were also an important source of pharmaceuticals to surface waters. Fifteen out of 59 OMPs have been found at levels posing a high risk for the aquatic IBAs ecosystems, being the insecticide chlorpyrifos, the antidepressant venlafaxine and perfluorooctanesulfonic acid (PFOS) the most concerning compounds. This is the first study to quantify water pollution in IBAs and evidence that OMPs are an emerging threat to freshwater ecosystems that are essential for biodiversity conservation.

Pilot monitoring scheme of water pollutants in Important Bird and Biodiversity Areas.

In this study we have established a monitoring scheme to determine the presence and distribution of widely used pharmaceuticals, pesticides, organophosphate esters (OPEs) and perfluoroalkyl substances (PFAS) in water bodies from Important Bird and Biodiversity Areas (IBAs) from Spain. The monitoring scheme included the georeferenced sampling of rocky mountain, Atlantic forest, riparian forest, Mediterranean forest, agricultural, inland aquatic and coastal aquatic IBAs, with the aim to evaluate the impact of widely used chemicals in those aquatic resources. Water samples were extracted using a generic solid-phase extraction protocol and analyzed by 3 analytical methods based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Quality parameters such as compound recovery, intra and inter-day variation, linearity and limits of detection were calculated in order to validate the methods. In addition, the ionization conditions and the optimization of the most appropriate transitions permitted unequivocal identification. Once the sampling and analytical procedure was set-up, 59 target compounds were monitored in 63 samples. Pharmaceutical, followed by pesticides, OPEs and PFAS were widespread along all IBAs studied at concentrations from 0.5 to 41083 ng/L. Overall, this study highlights the need to monitor the presence of contaminants in areas of high ecological interest to contribute to pollution control and mitigation towards protection of biodiversity.