Percutaneous hook wire assistance during laparoscopic excision of an intrarenal mass.

Completely endophytic renal tumours pose challenges in laparoscopic nephron-sparing tumour excisions, with the use of intraoperative imaging techniques (e.g. ultrasound) being crucial when managing such tumours. The use of a percutaneous hookwire for tumour localisations are in use in several other surgical fields, such as breast surgery. An asymptomatic 52-year-old man presented with an incidental small right sided solid 33-mm interpolar renal mass identified on computed tomography. A guided insertion of a percutaneous localisation wire was carried out prior to a laparoscopic partial nephrectomy to assist in intraoperative tumour landmark/margins identification. Operative time was 210 minutes with zero ischaemia time, with an estimated blood loss of 200 ml. No perioperative complications were observed and the patient was discharged two days postoperatively. Histology revealed the mass to be a Fuhrman grade 2 clear-cell carcinoma with a 2-mm clear surgical margin. The patient remained free of recurrence at 16 months of follow-up. We have reported our first experience of wire localisation prior to laparoscopic partial nephrectomy for an intrarenal mass, which to our knowledge could be the first of its kind in renal surgery. Percutaneous wire localisation of endophytic renal tumours is potentially safe and effective and can allow nephron-sparing surgery where laparoscopic ultrasound is not available. Longer-term and further evidence should be encouraged.

Evaluation of a small flat rectangular therapeutic ultrasonic transducer intended for intravascular use.

BACKGROUND: The aim of the proposed study was to evaluate the performance of a flat rectangular (2×10mm2) transducer operating at 4MHz. The intended application of this transducer is intravascular treatment of thrombosis and atherosclerosis. METHODS: The transducer's thermal capabilities were tested in two different gel phantoms. MR thermometry was used to demonstrate the thermal capabilities of this type of transducer. RESULTS: Temperature measurements demonstrated that this simple and small transducer adequately produced high temperatures, which can be utilized for therapeutic purposes. These high temperatures were confirmed using thermocouple and MR measurements. Pulsed ultrasound in combination with thrombolytic drugs and microbubbles was utilized to eliminate porcine thrombi. CONCLUSIONS: The proposed transducer has the potentials to treat atherosclerotic lesions using the thermal properties of ultrasound, since high temperatures can be achieved in less than 5s. The results revealed that the destruction of thrombi using pulsed ultrasound requires long exposure time and high microbubble dosage.

Distribution of heavy metals in sediment cores of Lake Pamvotis (Greece): a pollution and potential risk assessment.

Concentrations and vertical distributions of metals in surface sediments of Lake Pamvotis (NW Greece) were assessed using energy-dispersive X-ray fluorescence spectrometry. A wide range of values was determined, associated with the specific types of contaminating effluents draining into different parts of the lake. Overall, Cr levels ranged from 43 to 3295 mg kg(-1), Ni from 13 to 372 mg kg(-1), Cu from 15 to 24,985 mg kg(-1), Zn from 129 to 22,983 mg kg(-1), Zr from 64 to 4063 mg kg(-1) and Pb from 19 to 2634 mg kg(-1). Principal component analysis revealed distinct elemental fingerprints in each sampling location, while correlation analysis and hierarchical agglomerative cluster analysis provided insight to metal association and pollution sources. Enrichment factors and geoaccumulation indices were calculated to quantify sediment contamination, and potential ecotoxic effects were evaluated based on sediment quality guidelines. Moderate to very severe enrichment in Zn, Cu and Pb was evidenced in sediments near the lake's outflow. The proximity of these sampling points to a heavy traffic national road suggests that roadway runoff is the dominant source of elevated metal levels. Contribution from municipal sewage water discharges from the nearby communities should also be accounted for. Moderate to very severe Zn and Pb enrichment was determined in the vicinity on the main lake's inflow, primarily associated with runoff and leaching from agricultural land. Very severe to extremely severe Cr enrichment was detected in the same location, most likely associated with wastewater discharged into the lake over several decades, by nearby-operating leather tanneries. The above two locations were ranked at the highest priority level in terms of potential ecological risk.

Environmental measurements and inspections on imported foods and feedstuffs in Greece after the Fukushima accident.

The radionuclides released during the accident at the Fukushima Daichii nuclear power plant following the Tohoku earthquake and tsunami on 11 March 2011 were dispersed in the whole north hemisphere. Traces of (131)I, (134)Cs and (137)Cs reached Greece and were detected in air, grass, sheep milk, ground deposition, rainwater and drainage water. Members of Six Greek laboratories of the national network for environmental radioactivity monitoring have collaborated with the Greek Atomic Energy Commission (GAEC) and carried out measurements during the time period between 11 March 2011 and 10 May 2011 and reported their results to GAEC. These laboratories are sited in three Greek cities, Athens, Thessaloniki and Ioannina, covering a large part of the Greek territory. The concentrations of the radionuclides were studied as a function of time. The first indication for the arrival of the radionuclides in Greece originating from Fukushima accident took place on 24 March 2011. After 28 April 2011', concentrations of all the radionuclides were below the minimum detectable activities (<10 µBq m(-3) for (131)I). The range of concentration values in aerosol particles was 10-520 µBq m(-3) for (131)I, 10-200 µBq m(-3) for (134)Cs and 10-200 µBq m(-3) for (137)Cs and was 10-2200 µBq m(-3) for (131)I in gaseous phase. The ratios of (131)I/(137)Cs and (134)Cs/(137)Cs concentrations are also presented. For (131)I, the maximum concentration detected in grass was 2.2 Bq kg(-1). In the case of sheep milk, the maximum concentration detected for (131)I was 2 Bq l(-1). Furthermore, more than 200 samples of imported foodstuff have been measured in Greece, following the EC directives on the inspection of food and feeding stuffs.

Deconvolution of liquid scintillation alpha spectra of mixtures of uranium and radium isotopes.

A method for the determination of uranium and radium isotopes in water samples is proposed. Liquid scintillation techniques were used for collecting alpha spectra, which were then analyzed by fitting the alpha peaks with overlapping Gaussians. The analysis can quantify the observed isotopes with accuracy depending on the activity of each isotope. In order to simulate the peaks with Gaussian normal distribution functions, the centroid of each peak as well as the full width at half maximum (FWHM) are required, as they depend on the quenching of the sample. For this purpose, samples with known activities of 226Ra and its decay products and also of the uranium isotopes 238U and 234U, at various quenching levels, were used to establish the correlation of the peaks' shift with the quench effect. In addition, the correlation of the FWHM with the centroid of a peak was determined, using the same procedure. Following the above analysis technique, an average of 97+/-2% of detection efficiency and a lower limit of detection of 8.2 mBq kg(-1) for alpha isotopes were achieved.

The effect of moisture content on radon diffusion through soil: assessment in laboratory and field experiments.

The diffusion of radon through soil is strongly affected by the degree of water saturation of the soil pores. In the present work, a laboratory technique for studying radon diffusion has been developed and applied to determine diffusion coefficients in a sandy loam, containing various amounts of water, from null to saturation. The results indicate that, once the soil pore volume becomes saturated to values above approximately 20%, the diffusion of radon is markedly hampered; the bulk diffusion coefficient drops from 1.2 x 10(-6) to 2 x 10(-9) m2 s(-1) as soil saturation increases from 20 to 90%. The effect of soil moisture was further evaluated in field experiments conducted on soil of the same matrix. Comparison between results obtained by the two methods showed that laboratory studies may provide a good indication of radon diffusion coefficients to be expected in situ. However, values determined in the field were systematically lower than those assessed in the laboratory, illustrating the key role of structural differences between undisturbed and repacked soil.

Rapid screening of 90Sr activity in water and milk samples using Cherenkov radiation.

A method for screening 90Sr in milk samples is proposed. This method is based on a liquid scintillation technique taking advantage of Cherenkov radiation, which is produced in a liquid medium and then detected by the photomultipliers of a Liquid Scintillation Counter (LSC). Twenty millilitres of water and milk samples spiked with various concentrations of 90Sr/90Y in equilibrium were added in plastic vials and then were measured with an LSC (TriCarb 3170 TR/SL). The derived efficiencies were 49% for water samples and 14% for milk samples. The detection limit was 470 mBq L(-1)(90)Sr for water, without any pretreatment. Milk contains potassium, which also produces Cherenkov radiation due to the presence of 40K. For this reason, the interference of 40K in the measurements of 90Sr in milk samples was also investigated. The detection limit for milk was 1.7 Bq L(-1)90Sr.

Determination of 226Ra in aqueous solutions via sorption on thin films and alpha-spectrometry.

An improved spectrometric method to determine the 226Ra activity in aqueous solutions is described. The method involves two stages, a preconcentration stage of 226Ra sorption onto a thin manganese layer and a measurement stage using alpha-spectrometry. Manganese oxide thin films were prepared and characterized with X-ray diffraction (XRD) and X-ray fluorescence (XRF) analyses. The thin films were found to follow the XRD patterns and chemical formula of the K-birnessite layered exchanger. The preconcentration of radium was studied relative to the initial radium concentration, pH and salt concentrations. The preconcentration kinetics was studied as a function of manganese surface, solution volume and salt concentration. Extensive Monte Carlo calculations were performed to optimise the detection of alpha-particles. In this way, the thin film preparation procedure as well as the radium sorption and the measurement conditions were optimised and detection limits lower than 0.5 mBq L(-1) were obtained for 2d of procedure completion. The method was validated with IAEA standards and it was applied for the determination of 226Ra in bottled waters and also wastewaters from the major thermoelectric plant in Greece. Moreover, the 226Ra distribution coefficients (K(d)) of two differently prepared powder manganese oxides, a crystalline silicotitanate and an aluminium-pillared montmorillonite were determined by gamma-spectrometry. 226Ra sorption experiments on silicotitanate thin films were performed and improvements in resolution and reduction of exposure time were observed.

Variation in stakeholder opinion on countermeasures across Europe.

A compendium of agricultural countermeasures and rural waste disposal options has been compiled as part of the EC STRATEGY (Sustainable Restoration and Long-Term Management of Contaminated Rural, Urban and Industrial Ecosystems) project. The compendium was discussed by the FARMING (Food and Agriculture Restoration Management Involving Networked Groups) network of stakeholders during meetings of national panels in the UK, Finland, Belgium, Greece and France in 2002. Their preliminary feedback has been summarised in terms of whether an option is generally acceptable, unacceptable or only acceptable under specific circumstances. A considerable divergence of opinion between national panels was apparent for many of the options considered. This could be attributed to differences in geomorphology, climate, land management, infrastructure, consumer confidence, sociopolitical context and culture. Where consensus was reached between stakeholders it was generally for those countermeasures that provide public reassurance, sustain farming practices and minimise environmental impact. Furthermore, whilst there was general agreement that contaminated food should not enter the food chain, many of the options proposed for its subsequent management were not generally acceptable to stakeholders.

Achievements, difficulties and future challenges for the FARMING network.

The EC FARMING network (Food and Agriculture Restoration Management Involving Networked Groups) was set up to bring together the many and diverse stakeholders who would be involved in intervention following wide scale radioactive contamination of the food chain, so that acceptable strategies can be developed for maintaining agricultural production and safe food supply. The network comprises stakeholder panels in the UK, Finland, Belgium, France and Greece that have met regularly since 2001 to debate, discuss and exchange opinion on the acceptability, constraints and impact of various countermeasure options and strategies. The objectives of this paper are to consolidate the main achievements of the FARMING project over the period 2000-2004, to highlight the various difficulties that were encountered and to discuss the challenges for engaging stakeholders in off-site emergency management and long-term rehabilitation in the future.

Stakeholder involvement in nuclear crisis management in Greece.

The setting up of the Greek Stakeholders Group in the framework of the EC Food and Agriculture Restoration Management Involving Networked Groups (FARMING) project is described. The Group included members from more than 20 governmental and non-governmental organisations, having interest and/or responsibilities in the management of a crisis following a nuclear accident. The stakeholders, during their meetings in 2002, discussed the agricultural countermeasures and rural waste disposal options which have been compiled by the EC Sustainable Restoration and Long-Term Management of Contaminated Rural, Urban and Industrial Ecosystems (STRATEGY) project. All stakeholders agreed that the most preferable were those options that ensure public acceptance, minimise environmental impact and maintain farming practices and acceptable living and working conditions. Their views are synoptically presented along with the major conclusions from the stakeholders meetings regarding nuclear crisis management.

The effect of short-time microwave exposures on Escherichia coli O157:H7 inoculated onto chicken meat portions and whole chickens.

Small portions of fresh chicken breasts weighting 20 g each and fresh whole chickens, weighting on average 1310 g each, were inoculated with Escherichia coli O157:H7 (10(5)-10(6) cfu/g) and cooked, using two different domestic microwave ovens at full power. The chicken portions were heated for 5, 10, 15, 20, 25, 30, and 35 s and the whole chickens for 22 min. Following exposures, viable counts and temperature measurements were performed. Although the chicken breast portions looked well-cooked after 30 s of MW heating at a mean end-point surface temperature of 69.8 degrees C, a mean concentration of 83 cfu/g E. coli O157:H7 cells was recovered. Elimination of E. coli O157:H7 cells occurred only after 35 s of MW exposure at 73.7 degrees C. When whole chickens were thoroughly cooked by MW heating, the final subsurface temperatures, measured in the thighs and wings, ranged from 60.2 degrees C to 92 degrees C and viable cells of E. coli O157:H7 were recovered from all samples of whole chicken. The results indicate that short time exposures of chicken portions to microwave heating do not eliminate E. coli O157:H7.

Radon activity levels and effective doses in the Perama Cave, Greece.

An investigation of atmospheric radon levels in the Perama Cave, North-western Greece, has been carried out using CR-39 detectors. The detectors were placed at various locations along the guided cave pathway and exposed during different sampling periods. Mean concentrations amounting to 925 +/- 418 and 1,311 +/- 352 Bq m-3 were recorded in the summer and winter months, respectively. As the Perama Cave is one of the most popular in Greece, attracting more than 85,000 tourists per year, the quantification of effective doses to staff and visitors was an issue of importance. Doses less than 5.1 microSv per visit were calculated for tourists and around 1.8 mSv y-1 for seasonal guides, employed for periods of high visiting frequency. The annual exposure of permanent guides was estimated to fall between 3 and 10 mSv, which is the range of action levels recommended by the ICRP.

Soil gas radon: a tool for exploring active fault zones.

The profile of soil gas radon was monitored in five active fault sites in northern and northwestern Greece. Measurements were carried out during summer months, using CR-39 solid state nuclear track detectors (SSNTDs). The spatial distribution of radon along lines traversing the fault zones revealed anomalies, clearly connected to the local tectonic structure. Specifically, increased radon signals evolved on the radon background level, in the vicinity of the faults' axes and the signal-to-background ratio ranged from 2 to 13. The consistency of this pattern confirms that the radon technique is powerful in the detection and mapping of active fault zones.

Effect of soil amendments on radiocesium transfer to alfalfa.

Greenhouse experiments were carried out to evaluate the effect of different soil-based countermeasures on radiocesium transfer to Medicago saliva (alfalfa) grown on artificially contaminated loamy-clayey soil. Various rates of potassium, ammonium, and Prussian Blue supplements were applied, and the uptake of radiocesium by control and treated alfalfa plants was monitored during four growth periods. Transfer factors ranging between 0.06 and 0.02 were determined for control plants. Application of potassium at rates higher than 0.1 meq per 100 g soil was found to suppress radiocesium uptake, the effect being more pronounced at increasing fertilization rates. On the contrary, soil treatment with ammonium enhanced the bio-accumulation of radiocesium, indicating that Cs+ ions, previously unavailable to plant roots, were released from soil particles. Prussian Blue supplements had practically no effect on soil-to-alfalfa transfer of the radionuclide.

Use of HPGe gamma-ray spectrometry to assess the isotopic compositiion of uranium in soils.

Gamma-ray spectrometry was used to determine uranium activity and investigate the presence of depleted uranium in soil samples collected from camping sites of the Greek expeditionary force in Kosovo. Assessment of 238U concentrations was based on measurements of the 63.3 keV and 92.38 keV emissions of its first daughter nuclide, 234Th. To determine the isotopic ratio of 238U/235U, secular equilibrium along the two radioactive series was first ensured and thereby the contribution of 235U under the 186 keV peak was deduced. The uranium activity in the samples varied from 48 to 112 Bq kg(-1), whereas the activity ratio of 238U/235U averaged 23.1+/-4.3.

A survey of 222Rn concentrations in dwellings of the town of Metsovo in north-western Greece.

A radon survey has been carried out of indoor radon concentrations in dwellings located in the town of Metsovo, in north-western Greece. To measure indoor radon concentrations, CR-39 detectors were installed in randomly selected houses and were exposed for about 3 mo, during summer and winter. Gamma spectroscopy measurements of the soil's radium content also were performed. The indoor radon concentration levels varied from 17.6 to 750.4 Bq m(-3), while the radium concentration of soil varied from 4.9 to 97.1 Bq m(-3). Seasonal variation of the radon levels and the influence of house features and soil are discussed.

Strontium-90 concentration measurements in human bones and teeth in Greece.

Strontium-90 concentration was measured in human bones and teeth collected in Greece during the period 1992-1996. One hundred and five bone samples, mainly cancellous bone, and 108 samples, taken from a total of 896 individual teeth were processed. Samples were classified according to the age and sex of the donors. Samples were chemically pre-treated according to a specially devised method to enable extraction of 90Y, at equilibrium with 90Sr in the original sample. Subsequently, 90Y beta activity was measured with a gas proportional counter. Radiostrontium concentration in bone samples showed small variations with respect to age or sex, with an average value of 30 mBq 90Sr/g Ca. However, 90Sr concentration measurements in teeth demonstrated a pronounced structure, which clearly reflects contamination from the 1960s atmospheric nuclear weapons tests and the more recent Chernobyl accident. This difference is attributed to the different histological structure of skeletal bones and teeth, the later consisting mainly of compact bone. An age-dependent model for radiostrontium concentration in human bones and teeth is developed which is able to successfully reproduce the experimental data. Through a fitting process, the model also yielded calcium turnover rates for compact bone, as a function of age, as well as an estimate of radiostrontium contamination of foodstuffs in Greece for the past four decades. The results obtained in this study indicate that radiostrontium environmental contamination which resulted from the atmospheric nuclear weapons tests in the 1960s, exceed by far that caused by the Chernobyl accident.