[The dynamics of antibodies with IgD-class cardiolipin and treponemal group specificity in syphilis assessed quantitatively by radial immunodiffusion]. / Dinamica anticorpilor cu specificitate cardiolipinica si treponemica de grup din clasa IgD in sifilis, estimata cantitativ prin imunodifuzie radiala.

150 sera (positive at the VDRL, ELISA-Reiter, FTA-ABS tests) were tested by IDRS for the IgD quantification in syphilis. They were collected from men, 25-45 years old, in different stages of the disease, treated or not. The reference normal values for the seric IgD were established on 154 sera taken from men, 25-45 years old, apparently healthy: 0-131.2 UI/ml, with an average of 29.92 +/- 29.61 UI/ml. The IgD values with cardiolipin or group treponemal specificity were obtained from the difference between the values of the immunodiffusion diameters produced by sera, before and after the complete absorbtion with VDRL antigen or delipidated treponemal suspension. The individual values for each serum, mean +/- SD, and the percent values against the total IgD, for each stage of the disease were calculated. The medium levels of the total IgD range within normal limits, except for epsilon 2, where they are considerably higher than normal (52.53 +/- 26.66 UI/ml). All the individual minimal values, between 7.09 and 14.89 UI/ml, are higher than the normal minimal values, under 3.54 UI/ml. Treponemal IgD are present in all the sera in all stages of the disease and the cardiolipin IgD are completely absent. The mean values of the treponemal IgD are about 7-9 UI/ml, with a maximum of 19.3 UI/ml in epsilon 2. A higher percent of treponemal IgD is found, around 30%, with a maximum of 36.7% in epsilon 2. The high percent of the treponemal IgD in epsilon latent and epsilon treated persistent positive shows a continuous activation of the circulating B lymphocytes by the treponemal antigens and therefore an active infectious process. The exclusive presence of the treponemal IgD in all the cases of syphilis, irrespective of the evolution stages, indicated the extremely specific diagnosis value of their detection.

Quantitative profile of cardiolipin and group treponemal IgD antibodies in syphilis estimated by single radial immunodiffusion technique (SRID).

150 serum samples (reactive in VDRL, Reiter-ELISA, FTA-Abs tests), from male patients 25-45 years old, in various stages of syphilis whether treated or untreated, were tested for IgD by SRID. On 154 sera from healthy males 25-45 years old, the reference normal values for IgD levels were established, as: 0-131.2 IU/ml with a mean of 29.92 +/- 29.61 IU/ml. Cardiolipin and group treponemal fraction values for IgD class were obtained by assessing the difference between the immunodiffusion diameter values produced by sera before and after complete absorption with VDRL antigen or delipidated T. reiteri suspension. The individual, mean +/- SD values (expressed in IU/ml) and the percentage of cardiolipin and treponemal IgD of the total IgD class were calculated for each stage. The mean value of the total IgD class, excepting secondary syphilis (sigma 2) 52.53 +/- 26.66 IU/ml), did not overstep the normal levels but all minimal individual values from syphilitic patients (7.09-14.89 IU/ml) surpassed significantly the normal minimal values which were less than or equal to 3.54 IU/ml. The total lack of cardiolipin (IgD and the presence of group treponemal IgD in all sera of the syphilis stages studied were manifest. The group treponemal IgD mean values ranged between 7-9 IU/ml, with a maximum of 19.32 +/- 10.58 IU/ml in sigma 2 followed by latent syphilis (sigma lat) with a mean value of 9.37 +/- 4.9 IU/ml. A significant percentage of treponemal IgD vs total IgD was recorded: primary syphilis (sigma 1) 32.01%, primary-secondary syphilis (sigma 1-2) 28.76%, sigma 2 36.77%, sigma lat and treated persistent seroreactive syphilis (sigma t+) 29.61%. The high proportion of treponemal IgD in latent and treated persistent reactive syphilis suggests a steady activation of B lymphocytes by treponemal antigens and presumably is an expression of an active infectious process. The absence of cardiolipin IgD and the presence of only the treponemal IgD, in all sera from all stages, might confer to their detection an extremely specific diagnostic value in syphilis.

[Comparative study of immunofluorescence, coagglutination and urinary immunoglobulins in the diagnosis of the localization of urinary infections]. / Studiul comparativ al imunofluorescentei, coaglutinarii si imunoglobulinelor urinare în diagnosticul de localizare a infectiei urinare.

Three laboratory methods -- immunofluorescence, coagglutination and urinary immunoglobulin determinations -- were applied in 16 patients with urinary infections, of whom 12 with pyelonephritis and 4 with cystitis. There was an 100% agreement between the three tests in the pyelonephritis cases. In the cystitis cases, there was 100% agreement between immunofluorescence and urinary immunoglobulin and 50% between the three methods. Urinary immunoglobulin determination and coagglutination may be recommended as routine auxiliary clinical laboratory methods for early detection of renal lesions and localization of the infection.