High-pressure homogenization and high hydrostatic pressure processing of human milk: Preservation of immunological components for human milk banks.

Human milk (HM) constitutes the first immunological barrier and the main source of nutrients and bioactive components for newborns. Immune factors comprise up to 10% of the protein content in HM, where antibodies are the major components (mainly IgA, IgG, and IgM). In addition, antibacterial enzymes such as lysozyme and immunoregulatory factors such as soluble cluster of differentiation 14 (sCD14) and transforming growth factor ß2 (TGF-ß2) are also present and play important roles in the protection of the infant's health. Donor milk processed in HM banks by Holder pasteurization (HoP; 62.5°C, 30 min) is a safe and valuable resource for preterm newborns that are hospitalized, but is reduced in major immunological components due to thermal inactivation. We hypothesized that high hydrostatic pressure (HHP) and high-pressure homogenization (HPH) are 2 processes that can be used on HM to reduce total bacteria counts while retaining immunological components. We studied the effects of HHP (400, 450, and 500 MPa for 5 min applied at 20°C) and HPH (200, 250, and 300 MPa, milk inlet temperature of 20°C) applied to mature HM, on microbiological and immunological markers (IgA, IgG, IgM, sCD14, and TGF-ß2), and compared them with those of traditional HoP in HM samples from healthy donors. The HHP processing between 400 and 500 MPa at 20°C reduced counts of coliform and total aerobic bacteria to undetectable levels (<1.0 log cfu/mL) while achieving approximately 100% of immunological component retention. In particular, comparing median percentages of retention of immunological components for 450 MPa versus HoP, we found 101.5 versus 50.5% for IgA, 89.5 versus 26.0% for IgM, 104.5 versus 75.5% for IgG, 125.0 versus 72.5% for lysozyme, 50.6 versus 0.1% for sCD14, and 88.5 versus 61.1% for TGF-ß2, respectively. Regarding HPH processing, at a pressure of 250 MPa and inlet temperature of 20°C, the process showed good potential to reduce coliforms to undetectable levels and total aerobic bacteria to levels slightly above those obtained by HoP. The median percentages of retention of immunological markers for HPH versus HoP were 71.5 versus 52.0%, 71.0 versus 27.0%, 104.0 versus 66.5%, and 30.9 versus 0.2%, for IgA, IgM, IgG, and sCD14, respectively; results did not significantly differ for lysozyme and TGF-ß2. The HPH at 300 MPa produced higher inactivation of immunological components, similar to values achieved with HoP.

Objectively and subjectively measured physical activity and its relation to cardiovascular risk factors in older people-Which is most appropriate?

OBJECTIVES: To determine the strength of the association between objectively and subjectively measured physical activity (PA) and cardiovascular risk factors (CVRF) in a sample of older adults to assess which measure of PA is most appropriate for use in this population. STUDY DESIGN AND OUTCOME MEASURES: The study was a cross-sectional analysis of a community-dwelling sample aged 60 years or more (61 women and 34 men). Data collection included anthropometric measurements, blood analytics, and objective and subjective measurements of PA from accelerometers and Yale PA Survey questionnaires, respectively. RESULTS: Although the questionnaire indicated a higher summary index (total units) of PA in men than in women, objective measurements of total PA (mean counts per minute and steps per day) did not differ by sex. However, we obtained different results between women and men when we analyzed the relation between objective and subjective PA parameters and CVRF. In women, parameters that indicate cardiovascular risk, such as body mass index (BMI), waist circumference (WC), glucose, and total cholesterol, negatively correlated with objective PA parameters, while HDLc positively correlated with objective PA parameters and negatively with subjective PA parameters. In men, BMI and WC were negatively correlated with objective PA parameters, while blood cholesterol, LDLc, and triglycerides negatively correlated with subjective PA parameters. CONCLUSIONS: In women, it seems more appropriate to use objective methods to measure PA, while in men, objective and subjective methods seem to be complementary. Both methods of measurement, accelerometers and questionnaires, should be used simultaneously in research studies in older populations to establish precise relationships between PA and cardiovascular risk.

Physical activity and dietary habits related to cardiovascular risk in independent community-living older women.

A side effect of increased life expectancy is a surge in sequelae of diseases and injuries, which in turn increase the duration of life with disability among the elderly. The aim of this study was to ascertain the physical activity and nutritional parameters that better predict cardiovascular risk in a cohort of older women. A cross-sectional study was designed including 65 women able to independently perform basic activities of daily life. Data collection included anthropometric measurement, blood pressure measurement, blood analytics, objectively measurement of physical activity, and dietary assessment. We were able to generate models that explain the relationship between physical activity, diet, and these health measurement parameters. We observed that the combination of moderate physical activity and a diet including protein-rich foods as nuts, dairy, and eggs were better predictors associated with favorable changes in risk factors for cardiovascular disease than age.

Relationship between physical activity and markers of oxidative stress in independent community-living elderly individuals.

The aim of the present study was to examine the relationship between objective data of physical activity and markers of oxidative stress in older men and women. Participants were old adults, aged≥60years (61 women and 34 men) who were all capable of performing basic daily activities by themselves and lived on their own. To describe physical activity we used objective data measured by accelerometers which record active and sedentary periods during everyday life for five days. Determination of oxidative stress was conducted from three perspectives: determination plasma total antioxidant status (TAS), plasma antioxidant enzyme activities, i.e., glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD), and membrane lipid peroxidation (TBARS). In the group of women, those who met physical activity recommendations (WR) had lower level of TAS. In addition, the moderate to vigorous physical activity (MVPA) was negatively correlated with TAS. Simultaneously, MVPA was correlated with increase in the GPx antioxidant enzyme activity, and the counts per minute were positively correlated with CAT activity. In the group of men, the cpm and the MVPA were negatively correlated with lipid peroxidation while lifestyle physical activity was positively correlated with CAT activity. These findings suggest that MVPA in the elderly although it is related to a decrease in the TAS in women, induces adaptive increase in antioxidant enzyme activity and decreases lipid peroxidation in both women and men. These results suggest that at this time of life, it is not only the amount of physical activity performed that is important but also its intensity.

Longitudinal study: lifestyle and cardiovascular health in health science students.

BACKGROUND: Changing lifestyle habits is considered the principal measure for the control of blood pressure and obesity. The aim of this study was to characterize the eating habits, anthropometric characteristics, physical fitness and blood pressure of students of health science degrees during the first three academic years and to explore the relation between the aforementioned parameters. METHODS: This was a longitudinal study conducted over three years on the eating habits and physical fitness of health science students (n=366) and the influence of these factors on blood pressure and obesity. RESULTS: The mean food group intakes of both female and male participants corresponded to a high consumption of lean and fatty meat, sweets and pastries and a low consumption of cereals, fruits, vegetables, olive oil, fish, nuts and vegetables. Blood pressure and obesity-related parameters were within normal ranges and did not change over the study period. Aerobic capacity values increased in men from the beginning to the end of the study, while V02max decreased in women between the first and second years. CONCLUSION: We note that, in both women and men, blood pressure values were lower when diet was high in vegetables, legumes, nuts, fish and olive oil. In both sexes, we found a negative correlation between aerobic capacity and systolic and diastolic blood pressure and a positive correlation between obesity and blood pressure. The pattern of the eating habits and changes therein were different in young women and men, the former being focused on improving their diet and the latter on improving their physical fitness.

Objetivo: Cambiar los hábitos de vida se considera la principal medida para el control de la presión arterial y la obesidad. El objetivo de este estudio fue caracterizar los hábitos alimentarios, características antropométricas, condición física y la presión arterial de los estudiantes de grados de ciencias de la salud durante los tres primeros años académicos y analizar la relación entre los parámetros antes mencionados. Métodos: El estudio fue un estudio longitudinal llevado a cabo durante los tres primeros años de universidad, donde se midieron los hábitos alimenticios y la condición física de los estudiantes de grados de ciencias de la salud (n = 366) y la influencia de estos factores sobre la presión arterial y la obesidad. Resultados: El consumo de alimentos tanto de mujeres como de hombres, se correspondió con un consumo excesivo de carne magra y grasa, dulces y pasteles; y un bajo consumo de cereales, frutas, verduras, aceite de oliva, pescado, frutos secos y legumbres. Los parámetros de la presión arterial y de la obesidad se sitúan dentro de la normalidad y no varían a lo largo del periodo de estudio. Los valores de la capacidad aeróbica aumentaron en los hombres desde el principio hasta el final del estudio, mientras que V02max disminuyó en las mujeres entre el primer y segundo año. Conclusiones: Observamos que tanto en las mujeres como en los hombres, los valores de presión arterial fueron más bajos cuando la dieta era rica en verduras, legumbres, frutos secos, pescados y aceite de oliva. En ambos sexos, se encontró una correlación negativa entre la capacidad aeróbica y la presión arterial sistólica y diastólica y una correlación positiva entre la obesidad y la presión arterial. El patrón de los hábitos alimentarios y los cambios a lo largo de los tres años, fueron diferentes en las mujeres y los hombres. Las mujeres se centran más en la mejora de su dieta y los hombres en la mejora de su condición física.

Longitudinal study: lifestyle and cardiovascular health in health science students / Estudio longitudinal: estilos de vida y salud cardiovascular de estudiantes de ciencias de la salud

Background: Changing lifestyle habits is considered the principal measure for the control of blood pressure and obesity. The aim of this study was to characterize the eating habits, anthropometric characteristics, physical fitness and blood pressure of students of health science degrees during the first three academic years and to explore the relation between the aforementioned parameters. Methods: This was a longitudinal study conducted over three years on the eating habits and physical fitness of health science students (n=366) and the influence of these factors on blood pressure and obesity. Results: The mean food group intakes of both female and male participants corresponded to a high consumption of lean and fatty meat, sweets and pastries and a low consumption of cereals, fruits, vegetables, olive oil, fish, nuts and vegetables. Blood pressure and obesity-related parameters were within normal ranges and did not change over the study period. Aerobic capacity values increased in men from the beginning to the end of the study, while V02 max decreased in women between the first and second years. Conclusion: We note that, in both women and men, blood pressure values were lower when diet was high in vegetables, legumes, nuts, fish and olive oil. In both sexes, we found a negative correlation between aerobic capacity and systolic and diastolic blood pressure and a positive correlation between obesity and blood pressure. The pattern of the eating habits and changes therein were different in young women and men, the former being focused on improving their diet and the latter on improving their physical fitness (AU)

Objetivo: Cambiar los hábitos de vida se considera la principal medida para el control de la presión arterial y la obesidad. El objetivo de este estudio fue caracterizar los hábitos alimentarios, características antropométricas, condición física y la presión arterial de los estudiantes de grados de ciencias de la salud durante los tres primeros años académicos y analizar la relación entre los parámetros antes mencionados. Métodos: El estudio fue un estudio longitudinal llevado a cabo durante los tres primeros años de universidad, donde se midieron los hábitos alimenticios y la condición física de los estudiantes de grados de ciencias de la salud (n = 366) y la influencia de estos factores sobre la presión arterial y la obesidad. Resultados: El consumo de alimentos tanto de mujeres como de hombres, se correspondió con un consumo excesivo de carne magra y grasa, dulces y pasteles; y un bajo consumo de cereales, frutas, verduras, aceite de oliva, pescado, frutos secos y legumbres. Los parámetros de la presión arterial y de la obesidad se sitúan dentro de la normalidad y no varían a lo largo del periodo de estudio. Los valores de la capacidad aeróbica aumentaron en los hombres desde el principio hasta el final del estudio, mientras que V02 max disminuyó en las mujeres entre el primer y segundo año. Conclusiones: Observamos que tanto en las mujeres como en los hombres, los valores de presión arterial fueron más bajos cuando la dieta era rica en verduras, legumbres, frutos secos, pescados y aceite de oliva. En ambos sexos, se encontró una correlación negativa entre la capacidad aeróbica y la presión arterial sistólica y diastólica y una correlación positiva entre la obesidad y la presión arterial. El patrón de los hábitos alimentarios y los cambios a lo largo de los tres años, fueron diferentes en las mujeres y los hombres. Las mujeres se centran más en la mejora de su dieta y los hombres en la mejora de su condición física (AU)

Selection of young soccer players in terms of anthropometric and physiological factors.

AIM: The aim of this paper was to describe the anthropometric and physiological characteristics of young soccer players (14-17 years old) which were associated with their being successful or not as soccer players. METHODS: Somatotype and body composition was calculated by measuring skinfolds, limb circumferences and joint diameters. VO(2max) was estimated by the Astrand's Test. Sprint, jump and endurance tests were also performed. RESULTS: The most relevant differences were obtained between selected and non-selected players belonging to the 14-year-old team. Selected players were taller, heavier, leaner and faster and they had higher absolute or relative VO(2max). In addition, a higher % of selected players was found among those born during the first 6 months of the year. In the rest of the teams, the agility was better in selected than in non-selected players. At later ages, there was also a predominance of players born during the first 6 months of the year. CONCLUSIONS: These results indicate that around the time of puberty, parameters associated with physical maturity such as height, size, speed, VO(2max), or chronological age are important to determine the success of a soccer player. At older ages, other factors such as agility seem to be more important. Nevertheless, players born in the 1st semester of the year are also more frequent in the older teams. These findings should be taken into account by trainers and coaches, in order to avoid biasing their selection choices.

Effects of changes in hydromineral balance on rat brain aspartyl, arginyl, and alanyl aminopeptidase activities.

In order to examine the possible relationship between the processing and inactivation roles of aminopeptidases and the disruption of water-electrolyte balance, we measured the activities of aspartyl aminopeptidase (Asp-Ap), arginyl aminopeptidase (Arg-Ap) and alanyl aminopeptidase (Ala-Ap) in certain brain areas (hypothalamus, hippocampus, thalamus and brain cortices) and in the pituitary gland in several models of hydrosaline change. The activity of hypothalamic membrane-bound Asp-Ap significantly decreased (more than 50%) following treatments which induced a hypovolemic state. Aminopeptidase M activity (membrane-bound Ala-Ap activity with low sensitivity to puromycin) was also significantly decreased by 53 % in the thalamus of rats under conditions of hypovolemia plus hyperosmolality in comparison to the control group. These results indicate that aminopeptidases in the central nervous system may be involved in the physiological regulation of hydromineral balance.