Early detachment of prosthetic aortic valve one year after replacement for severe aortic regurgitation due to perforated aortic valve aneurysm; a case of suspected HLA-B52 positive Behçet's disease.

Herein, we report a case of 61-year-old male with congestive heart failure (HF) due to severe aortic regurgitation (AR) caused by ruptured aortic valve aneurysm (VA). Aortic valve replacement (AVR) was performed after improvement of HF symptoms. Pathological examination of resected aortic VA showed neutrophil infiltration. Although he did not have typical clinical features associated with infective endocarditis (IE) such as fever, leukocytosis, or positive blood culture, we misdiagnosed this case as "concealed IE". However, we reconsidered the etiology because prosthetic aortic valve detachment occurred only one year after AVR. When considering causes except IE for perforated aortic VA and early prosthetic valve detachment, Behçet's disease (BD) was more likely based on the clinical course, echocardiography, and pathological findings in this case. The inflammatory process of BD is associated with aortic valvulitis/aortitis, leading to a possible cause of aortic regurgitation due to aortic VA or early prosthetic valve detachment. The diagnosis of BD was challenging in this case because he did not have predominant clinical findings, including recurrent oral ulcer which is a mandatory criterion for the diagnosis by the International Study Group, however, cardiac involvement may have been the initial presentation of BD. .

Child ego state is associated with high prevalence of repeated hospitalizations in patients with heart failure.

AIMS: Inadequate self-care management is a leading cause of re-hospitalization in patients with heart failure (HF). Psychological factors such as some ego functions interfere with self-care behaviour modification, leading to poor outcomes in patients with several chronic diseases. However, characteristics of ego states in patients with repeated hospitalization for HF remain undefined. METHODS AND RESULTS: The present study enrolled 40 HF outpatients with previous history of HF hospitalization and receiving self-care management. Patients' psychological characteristics were assessed by Patient Health Questionnaire (PHQ-9) for screening depressive symptoms, and the Tokyo University Egogram (TEG) New Version II for analysing human behaviour based on five functional ego states; critical parent, nurturing parent, adult, free child, and adapted child (AC). Twelve patients (30.0%) had previous history of repeated (two or more) HF hospitalization. Most of them (75%) had a history of at least one or more re-hospitalizations related to inadequate self-care. Patients with repeated HF hospitalization had significantly lower AC score, which represents uncooperative and uncompromising behaviours, compared with those without repeated HF hospitalization (P < 0.05). There were no significant differences in other parameters, including PHQ-9, between the two groups. CONCLUSIONS: Low AC ego state was associated with high prevalence of repeated hospitalization in patients with HF. Assessing ego functions may be helpful to tailor educational approaches in these patients.

Antisense oligonucleotide-mediated exon skipping of CHRNA1 pre-mRNA as potential therapy for Congenital Myasthenic Syndromes.

CHRNA1 encodes the α subunit of nicotinic acetylcholine receptors (nAChRs) and is expressed at the neuromuscular junction. Moreover, it is one of the causative genes of Congenital Myasthenic Syndromes (CMS). CHRNA1 undergoes alternative splicing to produce two splice variants: P3A(-), without exon P3A, and P3A(+), with the exon P3A. Only P3A(-) forms functional nAChR. Aberrant alternative splicing caused by intronic or exonic point mutations in patients leads to an extraordinary increase in P3A(+) and a concomitant decrease in P3A(-). Consequently this resulted in a shortage of functional receptors. Aiming to restore the imbalance between the two splice products, antisense oligonucleotides (AONs) were employed to induce exon P3A skipping. Three AON sequences were designed to sterically block the putative binding sequences for splicing factors necessary for exon recognition. Herein, we show that AON complementary to the 5' splice site of the exon was the most effective at exon skipping of the minigene with causative mutations, as well as endogenous wild-type CHRNA1. We conclude that single administration of the AON against the 5' splice site is a promising therapeutic approach for patients based on the dose-dependent effect of the AON and the additive effect of combined AONs. This conclusion is favorable to patients with inherited diseases of uncertain etiology that arise from aberrant splicing leading to a subsequent loss of functional translation products because our findings encourage the option of AON treatment as a therapeutic for these prospectively identified diseases.

Pinhole balloon rupture and stuck stent: case report of a new and simple bailout technique for incomplete stent dilatation caused by rupture from a highly calcified lesion.

We report a case of highly calcified 75% stenotic lesion in the proximal left anterior descending coronary artery. During stenting, the balloon incurred a pinhole rupture at the moment of dilatation and the stent expanded only at both ends. This caused the balloon and stent to become lodged in the coronary artery. An attempt to dilate the balloon using rapid inflation alone was not successful, but we succeeded in dilating the stent with rapid balloon inflation by increasing the concentration of the contrast medium. We confirmed the occurrence of this phenomenon in vitro.

Does breastfeeding induce spontaneous abortion?

AIM: To verify the hypothesis that even if breastfeeding is continued during pregnancy, there are no harmful effects on pregnancy. METHODS: The outcomes of pregnancies in mothers who continued breastfeeding during pregnancy were studied. One hundred and ten pregnant women were recruited who had one full-term baby, no experience of premature birth or abortion, and who continued to breastfeed at diagnosis of pregnancy. Age and pregnancy history matched 774 pregnant women were selected as a control group. All of the control women stopped breastfeeding at least 3 months before becoming pregnant. We reviewed the outcome of each pregnancy retrospectively from patient records. Statistical analysis was conducted using the chi(2) test. RESULTS: Eight of 110 pregnancies (7.3%) resulted in spontaneous abortion in the breastfeeding group, and 65 of 774 pregnancies (8.4%) in the control group. There were no statistically significant differences between the two groups (P > 0.05). CONCLUSION: Even if the mother is pregnant, breastfeeding should be continued until natural weaning occurs. Further study is required to conclude safety of breastfeeding and premature birth.

Predictors and long-term prognostic implications of angiographic slow/no-flow during percutaneous coronary intervention for acute myocardial infarction.

OBJECTIVE: In patients with acute myocardial infarction (AMI), angiographic slow/no-flow during percutaneous coronary intervention (PCI) may lead to unfavorable outcomes. The aim of our study was to investigate the predictors and long-term prognosis of AMI patients with angiographic slow/no-flow. METHODS: We evaluated 228 consecutive AMI patients with either normal flow (Thrombolysis in Myocardial Infarction [TIMI] flow grade 3)(n=192) or slow/no-flow (< or =TIMI-2)(n=36) based on cineangiograms performed during PCI. RESULTS: Multivariable analysis demonstrated that a long lesion (> or =10 mm)(odds ratio [OR], 3.514; 95% confidence interval [CI], 1.505-8.206; p=0.004) and acute hyperglycemia (> or =180 mg/dl)(OR, 3.011; 95% CI, 1.211-7.485; p=0.018) were significant and independent predictors of angiographic slow/no-flow. Further, we found that there was a high correlation (89%) for predicting angiographic slow/no-flow when the optimal cut-off values of lesion length (10.45 mm) and blood glucose levels on admission (187.5 mg/dl) were combined as identified by analysis of the receiver operating characteristic curves. One-year mortality and incidence of major adverse cardiac and cerebrovascular events (MACCE) were significantly higher in the slow/no-flow group than that in the normal flow group. Angiographic slow/no-flow was independently predictive of MACCE (hazard ratio [HR], 3.642; 95% CI, 1.208-10.980; p=0.022) and cardiac death (HR, 5.287; 95% CI, 1.155-24.204; p=0.032). CONCLUSIONS: Lesion length and blood glucose level on admission can be used to stratify AMI patients into a lower or higher risk for angiographic slow/no-flow before optimal coronary intervention. In addition, angiographic slow/no-flow predicts an adverse outcome in AMI patients.

Comparison of the incidence of acute coronary event after balloon angioplasty vs. stenting in native coronary arteries.

OBJECTIVES: We sought to determine the incidence of acute coronary events after coronary stenting in comparison to that after plain old balloon angioplasty (POBA). BACKGROUND: Although coronary stenting is proven to be effective in reducing the incidence of restenosis, the long-term outcome in terms of acute coronary events has not been clarified. METHODS: Study subjects were 1,507 patients with 2,780 native coronary artery lesions treated by POBA (n=1,400) or stenting (n=1,380). Lesion stabilization, which was defined as freedom from restenosis confirmed by follow-up angiography 3 months after POBA and 6 months after stenting, was achieved in all subjects. All patients were clinically monitored for symptoms for at least 1 year after angioplasty, and the incidence of angiographically confirmed acute coronary event was compared between groups. RESULTS: The clinical follow-up period was significantly longer in the POBA group than in the stent group (8.4 +/- 5.0 years vs. 5.9 +/- 3.0 years, p<0.0001). Acute coronary events occurred in association with 32 lesions (1.2% overall); the incidence was 1.1% (15 of 1,400) after POBA and 1.2% (17 of 1,380) after stenting. Freedom from acute coronary events related to the treated lesions was similar between the two groups. (p=0.0518 by log-rank test). CONCLUSION: In terms of acute coronary events, the long-term clinical outcome of stenting is equivalent to that of POBA.