Asunto(s)
Hiperhidrosis , Yodo , Nevo , Enfermedades de las Glándulas Sudoríparas , Glándulas Ecrinas , Humanos , AlmidónAsunto(s)
Acrospiroma/diagnóstico , Biomarcadores de Tumor/metabolismo , Carcinoma Adenoide Quístico/diagnóstico , Neoplasias Complejas y Mixtas/diagnóstico , Neoplasias de las Glándulas Sudoríparas/diagnóstico , Acrospiroma/patología , Acrospiroma/cirugía , Anciano , Antígenos CD/análisis , Antígenos CD/metabolismo , Biomarcadores de Tumor/análisis , Biopsia , Carcinoma Adenoide Quístico/patología , Carcinoma Adenoide Quístico/cirugía , Folículo Piloso/citología , Folículo Piloso/metabolismo , Humanos , Queratina-15/análisis , Queratina-15/metabolismo , Pierna , Masculino , Neoplasias Complejas y Mixtas/patología , Neoplasias Complejas y Mixtas/cirugía , Células Madre/metabolismo , Neoplasias de las Glándulas Sudoríparas/patología , Neoplasias de las Glándulas Sudoríparas/cirugía , Glándulas Sudoríparas/patología , Glándulas Sudoríparas/cirugíaAsunto(s)
Livedo Reticularis/complicaciones , Pirazoles/uso terapéutico , Piridonas/uso terapéutico , Úlcera Cutánea/tratamiento farmacológico , Adulto , Tobillo , Femenino , Humanos , Livedo Reticularis/diagnóstico , Livedo Reticularis/tratamiento farmacológico , Livedo Reticularis/patología , Índice de Severidad de la Enfermedad , Piel/patología , Úlcera Cutánea/diagnóstico , Úlcera Cutánea/etiología , Úlcera Cutánea/patología , Resultado del TratamientoRESUMEN
A quantitative method for the determination of urea in serum was studied. An ion chromatograph (IC) with a conductivity detector was used in this method, where the chromatograph was modified by placing an immobilized urease column between the injection loop and a guard column of the cation analysis column. Immobilized urease was prepared by the adsorption of urease on cedar sawdust with triethylenetetramine. The adsorption capacity of urease was 190 mg g(-1), and its activity was 3500 U g(-1). The conversion efficiency of urea to ammonium ion was 100%, and the half life of immobilized urease was 60 days. It was possible to use the immobilized urease in a pH range of 3.0 to 9.0, and at temperatures up to 60 degrees C. The determination of urea was attempted by IC attaching an immobilized urease column. The limit of detection of urea was 0.2 mg L(-1), and the calibration curves of urea were very linear over 0.8-25 mg L(-1). The urea concentration in the human serum could be determined with a standard deviation of 0.06-0.13 within 5 min after injecting the serum sample.
