Association between endothelial nitric oxide synthase and the renin-angiotensin-aldosterone system polymorphisms, blood pressure and training status in normotensive/pre-hypertension and hypertensive older adults: a pilot study.

Introduction:Variations in blood pressure (BP) are, in part, genetically determined and some polymorphisms of renin-angiotensin- aldosterone system (RAAS) and synthase of endothelial nitric oxide (eNOS) have been related to hypertension (HT). Conversely, physical exercise is considered a non-pharmacological tool for HT control, treatment, and prevention.Objective: The purpose of this study is to investigate the relationship between eNOS and RAAS polymorphisms, their epistatic interaction, and the respective humoral factors in the BP control in normotensive/pre-hypertension and hypertensive older adults and how this relationship can be modulated by training status (TS) level.Methods:A total of 155 older adults (66.94 ± 6.83 years old) performed the following evaluations: AAHPERD battery test to determine the general functional fitness index (GFFI), systolic and diastolic blood pressure (SBP and DBP), blood collection for DNA extraction, analysis of eNOS gene polymorphisms rs2070744; rs61722009 and rs1799983 and RAAS polymorphisms rs699; rs1799752 and rs5186, and quantification of ACE activity (Fluorimetric Assay) and nitrite concentration (Chemiluminescence Method).Results and Conclusion:Good TS level appears to exert greater influence on SBP for G2 and G3 (G1: 125.79 ± 14.03/ G2: 119.91 ± 11.72/G3: 119.71 ± 10.85) and on NO2 for G3 (G1: 0.42 ± 0.25/ G2: 0.54 ± 0.45/ G3: 0.71 ± 0.52). No associations were observed between eNOS and RAAS polymorphisms, but the epistasis was identified between eNOS polymorphism, rs2070744, and RAAS polymorphism, rs699, revealing a statistically significant interaction (p = .0235) with training score of 0.63, a training test accuracy of 0.61 and a cross-validation consistency of 10/10. This result suggests an increased risk of hypertension.

Acute Exercise, Plasma Nitric Oxide, and Blood Pressure in Older Adults With Different Levels of Training Status: The Influence of Polymorphisms of Endothelial Nitric Oxide Synthase.

BACKGROUND: This study aimed to analyze the acute effect of physical exercise on nitric oxide concentration and blood pressure (BP) in older adults with different levels of training status (TS) and verified the influence of endothelial nitric oxide synthase polymorphisms on these variables. METHODS: A total of 145 older adults were divided into good TS (G1) and weak TS (G2). Participants were subjected to a 40-minute treadmill walk (40%-60% of maximum oxygen consumption) with BP measurements and blood collections for plasma nitrite and oxidative stress biomarkers at pretest and posttest moments. Data were analyzed by 2-way repeated-measures with Sidak post hoc test (P < .05) and multivariate linear analysis. RESULTS: After acute exercise, G2 showed an increase in oxidative stress biomarkers (P = .008), and both groups showed an increase in systolic BP (P < .001). Polymorphisms 894G > T and intron 4b/a had no association with nitrite and BP. However, -786T > C polymorphism showed an association with reduced systolic and diastolic BP (TT genotype) and increased diastolic BP (TC genotype). Higher TS level was also associated with lower BP. CONCLUSION: The maintenance of good TS levels may have a protective effect on cardiovascular risks regardless of the genetic profile.

Acute physical exercise and hypertension in the elderly: a systematic review / Exercício físico agudo e hipertensão arterial em idosos: revisão sistemática / Ejercicio físico agudo e hipertensión arterial en ancianos: revisión sistemática

ABSTRACT As the population ages, health conditions, including hypertension (HT), which is one of the most prevalent diseases in the elderly population, increase. Regular physical exercise has been recommended for hypertensive individuals; however, due to the variety of factors involved in exercise, different acute responses can be achieved. Accordingly, the purpose of this study was to perform a systematic review of the acute effect of physical exercise on blood pressure (BP) in elderly hypertensive patients and of its applicability to the treatment of HT. The search was performed in electronic databases available at Pubmed/Medline, Scopus and Bireme from 2008 to 2018, using the terms "acute physical exercise and hypertension and elderly". A total of 592 articles were found, and after applying the inclusion criteria, 9 articles were selected to form the analysis. All studies evaluated the acute effect of the exercise session and the acute effect of the session after a training period in male and female hypertensive individuals aged 60 years or over. The results indicate that in spite of the heterogeneity of training methods, all intervention protocols used in these studies were effective in promoting BP reduction post exercise when compared to the control group. However, there is still a gap in the reviewed literature regarding the maintenance time of post exercise hypotension (PEH) in the elderly. This information could suggest how long individuals undergoing physical exercise would be "protected" from high blood pressure values and their health risks, and help plan physical exercise sessions at the precise time the hypotensive effect ceases to be present. Level of evidence II; Therapeutic studies-Investigation of treatment results.

RESUMO À medida que a população envelhece, aumentam os agravos de saúde, entre eles, a hipertensão arterial (HA) destaca-se como uma das doenças de maior prevalência na população idosa. O exercício físico regular tem sido recomendado para indivíduos hipertensos, entretanto devido à variedade com que o exercício pode ser realizado, diferentes respostas agudas podem ser obtidas. Sendo assim, o objetivo do presente estudo consistiu em realizar uma revisão sistemática sobre o efeito agudo do exercício físico na pressão arterial (PA) em idosos hipertensos e sua aplicabilidade para o tratamento da HA. A busca foi realizada a partir de bancos de dados eletrônicos disponíveis no Pubmed/Medline, Scopus e Bireme de 2008 a 2018, utilizando os termos "acute physical exercise and hypertension and elderly". Foram encontrados 592 artigos e, após aplicar os critérios de inclusão, foram selecionados nove artigos que fizeram parte da análise, os quais avaliaram o efeito agudo da sessão de exercício e o efeito agudo da sessão após um período de treinamento em indivíduos hipertensos, com idade a partir de 60 anos, de ambos os sexos. Os resultados apontam que apesar da heterogeneidade nos métodos de treinamento, todos os protocolos de intervenção utilizados nesses estudos foram eficazes na promoção da redução da PA pós-exercício quando comparados ao grupo controle. No entanto, ainda existe uma lacuna na literatura revisada em relação ao tempo de manutenção da hipotensão pós-exercício (HPE) em idosos. Essa informação poderia sugerir por quanto tempo os praticantes estariam "protegidos" dos elevados valores pressóricos e seus riscos para a saúde e auxiliar no planejamento das sessões de exercício físico, justamente quando o efeito hipotensor estivesse deixando de se manifestar. Nível de evidência II; Estudos terapêuticos-Investigação dos resultados do tratamento.

RESUMEN A medida que la población envejece, aumentan los agravios de salud, entre ellos, la hipertensión arterial (HA) se destaca como una de las enfermedades de mayor prevalencia en la población anciana. El ejercicio físico regular ha sido recomendado para individuos hipertensos, sin embargo, debido a la variedad con que el ejercicio puede ser realizado, diferentes respuestas agudas pueden ser obtenidas. Siendo así, el objetivo del presente estudio consistió en realizar una revisión sistemática sobre el efecto agudo del ejercicio físico en la presión arterial (PA) en ancianos hipertensos y su aplicabilidad para el tratamiento de la HA. La búsqueda fue realizada a partir de bancos de datos electrónicos disponibles en el Pubmed/Medline, Scopus y Bireme de 2008 a 2018, utilizando los términos "acute physical exercise and hypertension and elderly".Se encontraron 592 artículos y, después de aplicar los criterios de inclusión, se seleccionaron nueve artículos que formaron parte del análisis, los cuales evaluaron el efecto agudo de la sesión de ejercicio y efecto agudo de la sesión después de un período de entrenamiento en individuos hipertensos, con edad a partir de 60 años, de ambos sexos. Los resultados apuntan que, a pesar de la heterogeneidad en los métodos de entrenamiento, todos los protocolos de intervención utilizados en estos estudios fueron eficaces en la promoción de la reducción de la PA postejercicio cuando comparados con el grupo control. Sin embargo, todavía existe un vacío en la literatura revisada con relación al tiempo de mantenimiento de la hipotensión postejercicio (HPE) en ancianos. Esta información podría sugerir por cuánto tiempo los practicantes estarían "protegidos" de los elevados valores presóricos y sus riesgos para la salud y auxiliar en la planificación de las sesiones de ejercicio físico, justamente cuando el efecto hipotensor estuviera dejando de manifestarse. Nivel de evidencia II; Estudios terapéuticos-Investigación de los resultados del tratamiento.

Association of endothelial nitric oxide synthase (eNOS) gene polymorphisms and physical fitness levels with plasma nitrite concentrations and arterial blood pressure values in older adults.

Endothelial nitric oxide synthase (eNOS) gene polymorphisms are associated with reduced eNOS activity and nitric oxide (NO) production leading to an increase in blood pressure (BP). Regular exercise is the main strategy to minimize the deleterious effects of polymorphisms. However, due to the differences that physical exercise can be performed, some controversial results are found. Therefore it seems reasonable to evaluate the training status (TS). Thus, this study aimed to investigate the association of eNOS gene haplotypes and different levels of TS on nitrite concentrations (NO2-) and BP values in older adult. 424 elderly performed the following assessments: General Functional Fitness Index (GFFI) to estimate TS, systolic and diastolic blood pressure (SBP and DBP), blood collection for analysis of NO2- and g.-786T>C, intron 4b/a (VNTR) and 894G>T polymorphisms. Multivariate logistic regression showed that NO2- was influenced by GFFI and 4b/4a Intron 4. Regarding BP, GFFI influenced SBP and DBP, and just intron 4 was associated with variations in DBP. It can be observed that GFFI affected the NO2-, SBP and DBP independently of haplotypes. Therefore, maintenance of good level of TS can overcome the negative influence of genetics factors (intron 4) by increasing NO2- concentration and decreasing BP values.

Association between insufficient practice of physical exercise, walking, and other modalities in healthy elderly / Associação entre a prática insuficiente de exercício físico, caminhada e outras modalidades em idosos saudáveis

ABSTRACT The objective was to compare functional capacity and health parameters of older adults with insufficient practice of physical exercise (IPPE) and older practitioners of different modalities of physical exercise. A cross-sectional study with 113 older people divided into the following groups: G1 - IPPE; G2 - walking practitioners; G3 - walking practitioners associated with one or more modalities; G4 - practitioners of one modality; and G5 - practitioners of two or more modalities. The AAHPERD test battery was used to evaluate functional capacity and the body fat percentage and blood pressure health parameters were analyzed. Analysis of Variance and the Odds Ratio were used. The results show differences at the level of p <0.05 in coordination and strength (G3> G1 and G4), agility (G2, G3 and G5 <G1 and G4), aerobic endurance (G3 and G5 <G1 and G4), the General Functional Fitness Index (G2> G1 and G3, G5> G1 and G4), and diastolic blood pressure (G1> G4 and G5). The practice of walking and practice of two or more physical exercise modalities are associated with better functional capacity in older adults.

RESUMO O principal objetivo foicomparar a capacidade funcional e parâmetros de saúde de idosos com prática insuficiente de exercício físico (IPPE) e idosos praticantes de diferentes modalidades de exercício físico. Trata-se de um estudo transversal, com 113 idosos, divididos nos seguintes grupos: G1 - IPPE; G2 -praticantes de caminhada; G3 - praticantes de caminhada associada a uma ou mais modalidades; G4 - praticantes de uma modalidade; G5 - praticantes de duas ou mais modalidades. A capacidade funcional foi avaliada pela bateria de testes da AAHPERD, e nos parâmetros de saúde foram analisados o percentual de gordura corporal e a pressão arterial. Para análise estatística utilizou-se Anova e OddsRatio. Os resultadosmostraramque diferenças ao nível de p<0,05 foram encontradas na coordenação e resistência de força, (G3 > G1 e G4) na agilidade (G2, G3 e G5 < G1 e G4) na resistência aeróbia (G3 e G5 < G1 e G4); no Índice de Aptidão Funcional Geral (G2 > G1 e G3; G5 > G1 e G4) e na pressão arterial diastólica (G1 > G4 e G5). A prática de caminhada e a prática de duas ou mais modalidades de exercícios físicos estão associadas a melhor aptidão funcional em idosos.

Exercício Físico Supervisionado, Aptidão Física e Fatores de Risco para Doenças Cardiovasculares em Adultos e Idosos / Supervised Physical Exercise, Physical Fitness and Risk Factors for Cardiovascular Diseases in Adults and Elderly

Objetivos: Verificar se o tempo de prática de exercícios físicos e a supervisão do profissional de Educação Física estão relacionados à melhores resultados na aptidão física e, investigar a relação entre aptidão física e fatores de risco para doenças cardiovasculares (DCVs) em adultos e idosos. Método: 213 participantes (idade entre 50 e 80 anos) realizaram as seguintes avaliações: índice de massa corporal (IMC), perfil lipídico sanguíneo, pressão arterial sistólica (PAS) e diastólica (PAD); e, aptidão física (bateria de testes motores que permitiu o cálculo índice de aptidão funcional geral (IAFG). A análise estatística foi realizada por meio de modelo linear generalizado (IAFG) e Análise de Variância (tempo de prática e supervisão). Resultados: Os dois grupos com tempo de prática de exercícios físicos >6 meses apresentaram melhores resultados no IAFG em comparação ao grupo com prática <6 meses (<6 meses ­ 228 (88,4) pontos; >6 meses sem supervisão - 274,6 (116,9) pontos; > 6 meses com supervisão - 355,2 (80,9) pontos), sendo possível observar resultados melhores para o grupo com supervisão em comparação ao grupo sem supervisão (p<0,05). Observou-se ainda diferenças entre os grupos quanto ao IAFG, para IMC, triglicerídeos, PAS e PAD, sendo que os grupos com maior IAFG apresentaram melhores resultados. Conclusão: Os melhores resultados do IAFG estão associados ao tempo de prática e à supervisão; bons níveis aptidão física estão associados com melhor saúde cardiovascular. Sugerem-se estratégias objetivando melhoras na aptidão física, destacando o profissional de Educação Física para uma prática eficaz e segura. (AU)

Objective: To determine whether the physical exercise time and the supervision by a physical education professional are related to better results in physical fitness. This study further investigated the relationship between physical fitness and risk factors for cardiovascular diseases (CVD) in adults and elderly people. Material and Methods: 213 participants (aged between 50 and 80 years) underwent the following assessments: variables related to risk factors for CVD - body mass index (BMI), blood lipid profile, systolic (SBP) and diastolic blood pressure (DBP); and factors related to physical fitness - motor test battery which allowed calculation of general functional fitness index (GFFI). Statistical analysis was performed using generalized linear model (GFFI) and Analysis of Variance (practice time and supervision). Results: The two groups with physical exercise time >6 months presented better results in the GFFI compared to the group with practice <6 months (<6 months - 228 (88.4) points; >6 months without supervision ­ 274.6 (116.9) points, >6 months with supervision - 355.2 (80.9) points, with better results for the group with supervision compared to the group without supervision (p <0.05). Higher values in GFFI groups were associated with better results in BMI, triglycerides, SBP and DBP. Conclusion: High values of GFFI are associated with the practice time and the supervision, and good levels of physical fitness are associated with better cardiovascular health. Strategies aiming at improvement in physical fitness are suggested, highlighting the physical education professional as a supervisor for an effective and safe practice. (AU)

Effect of Multicomponent Training on Blood Pressure, Nitric Oxide, Redox Status, and Physical Fitness in Older Adult Women: Influence of Endothelial Nitric Oxide Synthase (NOS3) Haplotypes.

The purpose of this study was to verify the influence of the genotype or haplotype (interaction) of the NOS3 polymorphisms [-786T>C, 894G>T (Glu298Asp), and intron 4b/a] on the response to multicomponent training (various capacities and motor skills) on blood pressure (BP), nitrite concentration, redox status, and physical fitness in older adult women. The sample consisted of 52 participants, who underwent body mass index and BP assessments. Physical fitness was evaluated by six-minute walk, elbow flexion, and sit and stand up tests. Plasma/blood samples were used to evaluate redox status, nitrite concentration, and genotyping. Associations were observed between isolated polymorphisms and the response of decreased systolic and diastolic BP and increased nitrite concentration and antioxidant activity. In the haplotype analysis, the group composed of ancestral alleles (H1) was the only one to present improvement in all variables studied (decrease in systolic and diastolic BP, improvement in nitrite concentration, redox status, and physical fitness), while the group composed of variant alleles (H8) only demonstrated improvement in some variables of redox status and physical fitness. These findings suggest that NOS3 polymorphisms and physical training are important interacting variables to consider in evaluating redox status, nitric oxide availability and production, and BP control.

Training Status as a Marker of the Relationship between Nitric Oxide, Oxidative Stress, and Blood Pressure in Older Adult Women.

The purpose of this study was to evaluate the influence of functional fitness and oxidative capacity on the nitric oxide concentration associated with hemodynamic control in older adult women. The sample consisted of 134 women (65.73 ± 6.14 years old). All subjects underwent a physical examination to assess body mass index, waist-hip ratio, body fat measurement by dual energy X-ray absorptiometry, and blood pressure (BP). Training status (TS) was evaluated by indirect determination of maximal oxygen uptake by a treadmill test using Balke protocol modified for older adults. Functional fitness was also evaluated through a "Functional Fitness Battery Test" to determine the general fitness functional index (GFFI). All participants were separated according to the functional fitness (TS1, very weak and weak; TS2, regular; TS3, good and very good). Plasma blood samples were used to evaluate prooxidant and antioxidant activity and nitrite and nitrate concentrations. The general results of this study showed that good levels of TS were related to lower levels of lipoperoxidation and protein damage, higher levels of antioxidant, and higher concentration of nitrite and nitrate. This combination may be responsible for the lower levels of BP in subjects with better TS.

The relationship between training status, blood pressure and uric acid in adults and elderly.

BACKGROUND: Hypertension can be generated by a great number of mechanisms including elevated uric acid (UA) that contribute to the anion superoxide production. However, physical exercise is recommended to prevent and/or control high blood pressure (BP). The purpose of this study was to investigate the relationship between BP and UA and whether this relationship may be mediated by the functional fitness index. METHODS: All participants (n = 123) performed the following tests: indirect maximal oxygen uptake (VO2max), AAHPERD Functional Fitness Battery Test to determine the general fitness functional index (GFFI), systolic and diastolic blood pressure (SBP and DBP), body mass index (BMI) and blood sample collection to evaluate the total-cholesterol (CHOL), LDL-cholesterol (LDL-c), HDL-cholesterol (HDL-c), triglycerides (TG), uric acid (UA), nitrite (NO2) and thiobarbituric acid reactive substances (T-BARS). After the physical, hemodynamic and metabolic evaluations, all participants were allocated into three groups according to their GFFI: G1 (regular), G2 (good) and G3 (very good). RESULTS: Baseline blood pressure was higher in G1 when compared to G3 (+12% and +11%, for SBP and DBP, respectively, p<0.05) and the subjects who had higher values of BP also presented higher values of UA. Although UA was not different among GFFI groups, it presented a significant correlation with GFFI and VO2max. Also, nitrite concentration was elevated in G3 compared to G1 (140±29 µM vs 111±29 µM, for G3 and G1, respectively, p<0.0001). As far as the lipid profile, participants in G3 presented better values of CHOL and TG when compared to those in G1. CONCLUSIONS: Taking together the findings that subjects with higher BP had elevated values of UA and lower values of nitrite, it can be suggested that the relationship between blood pressure and the oxidative stress produced by uric acid may be mediated by training status.