Multifunctional and stimuli-responsive liposomes in hepatocellular carcinoma diagnosis and therapy.

Hepatocellular carcinoma (HCC) is the most prevalent type of liver cancer, mainly occurring in Asian countries with an increased incidence rate globally. Currently, several kinds of therapies have been deployed for HCC therapy including surgical resection, chemotherapy, radiotherapy and immunotherapy. However, this tumor is still incurable, requiring novel strategies for its treatment. The nanomedicine has provided the new insights regarding the treatment of cancer that liposomes as lipid-based nanoparticles, have been widely applied in cancer therapy due to their biocompaitiblity, high drug loading and ease of synthesis and modification. The current review evaluates the application of liposomes for the HCC therapy. The drugs and genes lack targeting ability into tumor tissues and cells. Therefore, loading drugs or genes on liposomes can increase their accumulation in tumor site for HCC suppression. Moreover, the stimuli-responsive liposomes including pH-, redox- and light-sensitive liposomes are able to deliver drug into tumor microenvironment to improve therapeutic index. Since a number of receptors upregulate on HCC cells, the functionalization of liposomes with lactoferrin and peptides can promote the targeting ability towards HCC cells. Moreover, phototherapy can be induced by liposomes through loading phtoosensitizers to stimulate photothermal- and photodynamic-driven ablation of HCC cells. Overall, the findings are in line with the fact that liposomes are promising nanocarriers for the treatment of HCC.

Multifunctional and theranostic hydrogels for wound healing acceleration: An emphasis on diabetic-related chronic wounds.

Hydrogels represent intricate three-dimensional polymeric structures, renowned for their compatibility with living systems and their ability to naturally degrade. These networks stand as promising and viable foundations for a range of biomedical uses. The practical feasibility of employing hydrogels in clinical trials has been well-demonstrated. Among the prevalent biomedical uses of hydrogels, a significant application arises in the context of wound healing. This intricate progression involves distinct phases of inflammation, proliferation, and remodeling, often triggered by trauma, skin injuries, and various diseases. Metabolic conditions like diabetes have the potential to give rise to persistent wounds, leading to delayed healing processes. This current review consolidates a collection of experiments focused on the utilization of hydrogels to expedite the recovery of wounds. Hydrogels have the capacity to improve the inflammatory conditions at the wound site, and they achieve this by diminishing levels of reactive oxygen species (ROS), thereby exhibiting antioxidant effects. Hydrogels have the potential to enhance the growth of fibroblasts and keratinocytes at the wound site. They also possess the capability to inhibit both Gram-positive and Gram-negative bacteria, effectively managing wounds infected by drug-resistant bacteria. Hydrogels can trigger angiogenesis and neovascularization processes, while also promoting the M2 polarization of macrophages, which in turn mitigates inflammation at the wound site. Intelligent and versatile hydrogels, encompassing features such as pH sensitivity, reactivity to reactive oxygen species (ROS), and responsiveness to light and temperature, have proven advantageous in expediting wound healing. Furthermore, hydrogels synthesized using environmentally friendly methods, characterized by high levels of biocompatibility and biodegradability, hold the potential for enhancing the wound healing process. Hydrogels can facilitate the controlled discharge of bioactive substances. More recently, there has been progress in the creation of conductive hydrogels, which, when subjected to electrical stimulation, contribute to the enhancement of wound healing. Diabetes mellitus, a metabolic disorder, leads to a slowdown in the wound healing process, often resulting in the formation of persistent wounds. Hydrogels have the capability to expedite the healing of diabetic wounds, facilitating the transition from the inflammatory phase to the proliferative stage. The current review sheds light on the biological functionalities of hydrogels, encompassing their role in modulating diverse mechanisms and cell types, including inflammation, oxidative stress, macrophages, and bacteriology. Additionally, this review emphasizes the significance of smart hydrogels with responsiveness to external stimuli, as well as conductive hydrogels for promoting wound healing. Lastly, the discussion delves into the advancement of environmentally friendly hydrogels with high biocompatibility, aimed at accelerating the wound healing process.

Effect of shortening the dry period on colostrum and milk quality, blood parameters and some reproductive parameters in high-producing Holstein cows of different body condition score.

This study aimed to evaluate the effect of shortening the dry period in high-yielding cows of different body condition scores (BCS). We report colostrum and milk quality, some serum metabolites, BCS changes, and some reproductive parameters with measurements being made over the first two months of lactation. Cows were grouped based on the length of the dry period (normal: about 50 d and short: about 28 d) and BCS (moderate: 2.75 to 3.5 and high ≥ 3.5). Short dry period decreased colostrum volume and, in combination with high BCS only, caused a decrease in milk production. Short dry period moderate BCS cows had the highest serum insulin concentration on day 14 after calving and highest glucose concentration on day 28, but neither differed significantly when measured over the whole period. By contrast, short dry period cows had significantly lower concentrations of non-esterified fatty acids and beta-hydroxybutyrate measured over the whole period. Post-partum loss of BCS was less in short and especially so in the short, moderate BCS group. Following a synchronization protocol at 35 d postpartum. The cows with a short dry period and moderate BCS had lower open days, days to first postpartum estrus and services per conception. It was concluded that short dry periods and moderate BCS had a positive influence on serum metabolites, BCS changes and reproductive parameters.

Effect of dry period length on plasma minerals and oxidative stress around parturition and milk yield in high-producing Holstein dairy cows.

The aim of present study was to evaluate the effect of dry period length (56 vs. 28 days) on plasma minerals, albumin, total protein and globulin, oxidative status, and milk production in high-producing dairy cows (13,500 ± 1800 kg milk in previous 305-day lactation). A total of 120 multiparous Holstein cows were used in a repeated measurement mixed model (animal was considered random effect) with two experimental groups and 60 replicates each: 1-long dry period (LDP, 56-day dry period) and 2-short dry period (SDP, 28-day dry period). While LDP cows received two different rations in far-off and close-up periods (DCAD = 189 and - 130 Meq/Kg, respectively), the SDP cows received only the close-up ration (- 130 Meq/Kg) without passing the far-off stage. Blood samples for minerals, oxidative stress, and metabolites (albumin, total protein) were collected on calving day, 24 and 48 h after parturition. Milk samples of all cows in each group were collected monthly (four months from parturition to 120 DIM) and analyzed for milk fat, protein, and somatic cell count (SCC). Results showed that SDP had no effect on plasma calcium (Ca), ionized calcium (iCa), magnesium (Mg), and phosphorus (iP). Total antioxidant capacity (TAC) in the SDP cows was higher (P < 0.05) than the LDP cows. The treatment had no significant effect on plasma concentration of total protein, albumin, globulin, and superoxide dismutase (SOD) activity. A positive significant (P < 0.05) correlation was observed between plasma Mg and TAC in SDP group. Short dry period reduced (P < 0.05) milk production (48.39 vs. 51.95 kg/day; about 7%) during the first 120 days in milk. However, SDP cows had higher (P < 0.05) milk protein content compared to LDP group. The log-transformed of somatic cell count (LnSCC) was not affected by the treatment. It was concluded that a short dry period (28 days) had no negative effect on plasma Ca, iCa, Mg, and iP and even could improve TAC in high-producing Holstein cows.