Characterization of metabolites in Saposhnikovia divaricata root from Mongolia.

Saposhnikoviae Radix (SR), derived from the dried root and rhizome of Saposhnikovia divaricata, is a popular crude drug used in traditional Chinese and Japanese medicine. To evaluate the metabolites of S. divaricata roots from Mongolia and to investigate their geographical variation, we developed the HPLC method, determined the contents of 9 chromones and 4 coumarins, and conducted multivariate statistical analysis. All Mongolian specimens contained prim-O-glucosylcimifugin (1) and 4'-O-ß-D-glucosyl-5-O-methylvisamminol (3), and their total amount (5.04-25.06 mg/g) exceeded the criterion assigned in the Chinese Pharmacopoeia. Moreover, the content of 1 (3.98-20.79 mg/g) was significantly higher in the Mongolian specimens than in Chinese SR samples. The specimens from Norovlin showed the highest contents of 1 and 3. The total levels of dihydropyranochromones were higher in the specimens from Bayan-Uul. The orthogonal partial least squares-discriminant analysis revealed that the Mongolian specimens tended to be separated into three groups based on growing regions, in which several chromones contributed to each distribution. Furthermore, 1H NMR analysis revealed that Mongolian specimens had less amount of sucrose and a substantial amount of polyacetylenes. Thus, in this study, the chemical characteristics of Mongolian S. divaricata specimens were clarified and it was found that the specimens from the northeast part of Mongolia, including Norovlin, had the superior properties due to higher amounts of major chromones.

Isolated compounds from Saxifraga Spinulosa and their antioxidative activity / Монголын эм зүй, эм судлал

Abstract@#The genus <i>Saxifraga</i>, one of the largest genera of the Saxifragaceae family, comprises 540 species and is distributed widely in mountainous and rocky regions, with 12 species being found in Mongolia. <i>Saxifraga spinulosa</i> Adams is a perennial herbaceous plant common in mainland China, Russia, and Mongolia, inhabiting stony marginal terrain. The isolated compounds from <i>Saxifraga spinulosa</i> were screened for DPPH radical-scavenging activity, with Trolox as a positive control (IC₅₀ 23.3 µM). All the new glucosides exhibited potent activities (IC₅₀ 19.0–72.9 µM). A crude ex- tract of <i>S. spinulosa</i> has been reported to display the highest DPPH radical-scavenging activity among numerous Mongolian medicinal plants, which may now be attributed, at least in part, to the presence of the new flavonoid and galloyl group-containing isolated compounds.

Metabolomic profiling of Saposhnikoviae Radix from Mongolia by LC-IT-TOF-MS/MS and multivariate statistical analysis.

Saposhnikoviae Radix (SR) is a commonly used crude drug that is obtained from the root and rhizome of Saposhnikovia divaricata which is distributed throughout China, Korea, Mongolia, and Russia. To evaluate the quality of Mongolian S. divaricata, metabolomic profiling of 43 plant specimens from different regions of Mongolia, as well as 8 SR samples and 2 plant specimens from China, were conducted by liquid chromatography-ion-trap-time-of-flight-mass spectrometer (LC-IT-TOF-MS). LC-MS profiles of the specimens showed uniformity and 30 compounds were tentatively identified, including 13 chromones and 17 coumarins. Among them, 16 compounds were isolated and unambiguously verified by comparing them with the spectroscopic data of standard compounds. Orthogonal partial least squares-discriminant analysis (OPLS-DA) based on LC-MS data from 7 Mongolian specimens and 8 Chinese SR samples as well as 2 plant specimens revealed that these 2 groups were clearly distinguishable and that Mongolian specimens were characterized by an abundance of prim-O-glucosylcimifugin (1). Moreover, the OPLS-DA of the Mongolian specimens showed that they can be discriminated by their growing regions based on the content of 8 chromones. The total content of dihydrofurochromones 1-3 was relatively higher in the specimens from Khalkhgol in the far eastern part of Mongolia, while contents of 10, 11, 15, and 16 were higher in those from Holonbuir in the eastern part. Based on this research, the roots of S. divaricata from Mongolia have potential as a new resource of SR in Kampo medicine.

Astragalus root induces ovarian ß­oxidation and suppresses estrogen­dependent uterine proliferation.

Continuous estrogen stimulation in the uterus has been known to cause excess proliferation of the functional layer of endometrium, resulting in endometrial hyperplasia and leading to infertility. Estrogens can modulate other nuclear receptor signaling pathways, such as peroxisome proliferator­activated receptors (PPARs). Astragalus root (AsR) has exhibited strong PPARα agonistic activity. Female Imprinting Control Region mice were fed a powder diet that included 5% AsR hot water extract or 0.1% bezafibrate as a positive control for 56 days to investigate AsR effects on the reproductive tract, ovary and uterus. AsR resulted in upregulation of the expression of uterine and ovarian PPARα mRNA by 2.5­fold, and 1.5­fold, respectively, compared with controls. AsR significantly increased ovarian expression levels of mitochondrial 2,4­dienoyl­CoA reductase (mDECR), an auxiliary enzyme involved in ß­oxidation. AsR­fed mice also exhibited a significant increase in blood estradiol levels and tended to have higher ovary weight. AsR resulted in significantly decreased uterine weight and mDECR expression levels. It has been reported that a PPARα agonist suppresses the development of estrogen­dependent endometrial hyperplasia. These findings raise the possibility that AsR suppresses estrogen­dependent endometrial hyperplasia and ovarian dysfunction leading to infertility.

Antigenotoxic and antioxidant effects of the Mongolian medicinal plant Leptopyrum fumarioides (L): an in vitro study.

ETHNOPHARMACOLOGICAL RELEVANCE: Leptopyrum fumarioides has been used in the traditional medicine of Mongolia for the treatment of various diseases, including drug intoxications. However, since there is only sparse information about its chemistry, active components, and pharmacological and toxicological effects, the major aim of the present study employing mouse lymphoma cells was to evaluate the genotoxic and antigenotoxic/antioxidative effects of extracts and components isolated from this plant. MATERIAL AND METHODS: A crude methanol extract was separated into three different sub-extracts: dichloromethane, n-butanol, and water. The major constituent of the n-butanol extract, i.e., the flavone luteolin-7-O-glucoside and a mixture of the most abundant compounds in the dichloromethane sub-extract were then isolated. DNA damage was evaluated using the comet assay; the antioxidant activity was evaluated using the DPPH radical scavenging assay. RESULTS: The crude methanol extract, the dichloromethane sub-extract and the mixture of compounds isolated from the latter fraction, increased the level of DNA damage after three hours of exposure. In contrast, no increase in DNA damage was observed in the cells that had been exposed to the n-butanol and water sub-extracts, or to the pure flavone. When non-DNA damaging concentrations of extracts and compounds were tested together with the DNA damaging agent catechol, all sub-extracts were found to reduce the catechol-induced DNA damage (the flavone was then found to be the most effective protective agent). The n-butanol sub-extract and the flavone were also found to have the most prominent antioxidative effects. CONCLUSION: Based on the results from the present study, components in Leptopyrum fumarioides were found to protect the DNA damage induced by catechol, probably by acting as potent antioxidants.

Mongolian Traditional Medicine and Medicinal Plants / 日本東洋医学雑誌

We visited Mongolia in the summer of 1999 and again in the summer of 2000, and investigated the traditional and natural medicines of this country. Mongolian traditional medicine is classified into seven categories, namely, massage, acupuncture, herbal medicine, dietary cures, aromatherapy, phlebotomy and sutra recitation. Herbal medicines, as well as acupuncture and massage, are the principal remedies. These remedies are based on Tibetan traditional medicine, which is derived from Indian traditional medicine. In both rural and urban areas, the Mongolian people use many herbs and herbal prescriptions to prevent and cure acute or chronic diseases. Important herbal plant sources, such as the <i>Ephedra</i> and <i>Glycyrrhiza</i> species, are abundant. Since the abandonment of socialism, traditional medicine has made a rapid comeback and is now a popular and important remedy in Mongolia.