Molecular cytogenetic analysis of the olive flounder embryonic cell line FGBC8 and its applicability to biotechnology.

We explored the biotechnological applicability of a previously established olive flounder (Paralichthys olivaceus) embryonic cell line (FGBC8). FGBC8 was transfected with pEGFP-c1 and pluripotency-related genes, then infected with viral hemorrhagic septicemia virus (VHSV), and the expression of immune-related genes was observed through quantitative real-time polymerase chain reaction. Transfected cells showed strong green fluorescence 48 h after transfection, and pluripotency-related genes were successfully transfected. In addition, FGBC8 cells were highly susceptible to VHSV and the expression of immune-related genes was induced during infection. Our results demonstrate that FGBC8 cells are valuable research tools for assessing host-pathogen interactions and biotechnological applications.

The complete mitochondrial genome of the blue runner, Caranx crysos (Mitchill, 1815) (Teleostei: Carangidae).

Caranx crysos was collected from offshore of Sierra Leone and its complete mitochondrial genome was determined using next-generation sequencing (NGS). The circular mitogenome encoded a typical 37 genes, including 13 protein-coding genes (PCGs), 2 ribosomal RNA genes (12S rRNA and 16S rRNA), and 22 tRNA genes. An unusual start codon (GTG) was identified for the COX1 gene, and incomplete stop codons (T-/TA-) were found in seven genes, including ND2, ND3, ND4, COX2, COX3, ATP6, and CytB. All tRNAs were predicted to fold into the typical clover-leaf structures, except for tRNASer-GCT, which lacks the D-arm. C. crysos formed a monoclade with the tree other species belonging to the genus Caranx, apart from the others. Among them, C. crysos was most closely related to Caranx melampygus and Caranx tille. The mitogenome sequence of C. crysos provides information for a better understanding of evolutionary relationships, systemic, and mitogenomic study within the family Carangidae.