Postsurgical status of articular cartilage after arthroscopic posterior cruciate ligament reconstruction in patients with or without concomitant meniscal pathology.

PURPOSE: Several factors present at the time of posterior cruciate ligament reconstruction (PCLR) may cause the subsequent progression of articular cartilage lesions. This study aimed to evaluate postsurgical articular cartilage lesions which can be seen on MRI in patients who underwent arthroscopic PCLR with or without concomitant meniscal pathology. MATERIAL AND METHODS: A total of sixty-five patients (mean age 35.8 ± 12.3 years) who underwent arthroscopic PCLR were included in this retrospective study. Patients were divided into two groups: ten patients with concomitant meniscal injuries at the time of PCLR who underwent meniscal surgery and fifty-five patients with intact menisci. The cartilage status of all knees was evaluated by MRI and modified Noyes classification. RESULTS: Cartilage lesions were observed in 18 patients (27.7%) on the last follow-up MRI. The cartilage lesions were more common in the medial (15.4%) and patellofemoral (12.3%) compartments than in the lateral compartment (7.7%). Progression of cartilage lesions was present in 11 patients (16.9%) during follow-up MRI. The majority of cartilage lesions with progression were located in the medial compartment. The meniscal pathology group showed a higher prevalence of articular cartilage lesions on the last follow-up MRI (21.8% versus 60%, p = 0.022). In multivariate Cox regression, concomitant meniscal pathology was significantly associated with progression of articular cartilage lesions (p = 0.044). CONCLUSION: PCLR patients with associated meniscal pathology showed worse cartilage condition and more progression of cartilage lesions than isolated PCLR patients. Attention to this risk factor might provide more applicable treatment options for potential osteoarthritis prevention strategies.

Radiation dose reduction in plain radiography of the full-length lower extremity and full spine.

OBJECTIVE: To compare the diagnostic performance of standard- and low-dose radiographs of the full-length lower extremity and spine. METHODS: This study included 223 patients who visited our hospital and received full-length lower extremity standing radiographs and full-spine radiographs. We determined the dose area product (DAP) of each image, and effective doses (ED, mSv) were calculated based on the DAP. Subjective evaluation of the full-length radiographs was based on image quality, which was assessed by bony cortex and trabecula evaluation, and on diagnostic performance, which was assessed by leg length measurement. Subjective evaluation of the full-spine radiographs was based on image quality, which was assessed by viewing the vertebral endplate, pedicle and lateral border of vertebral body, and on diagnostic performance from measurement of Cobb's angle. RESULTS: For the full-length view and the full-spine view both the mean DAP and ED values of the standard-dose group were significantly higher than those of the low-dose group (p < 0.05). Mean scores for subjective values did not significantly differ based on the radiation dosage (p-values, 0.15-0.99). The subjective value scores for the full-length view were 2.94-2.98 in the standard-dose group and 2.91-3.00 in the low-dose group. Of note, both groups had very high scores. Additionally, the diagnostic performance scores between the two groups were also very high (range from 2.92 to 3.00). CONCLUSION: Reducing mAs by 50% of the standard dose does not affect the radiograph image quality or its clinical validity. Advances in knowledge: Radiation dose reduction (50% of the standard dose of mAs) in plain radiography of the full-length lower extremity and full spine do not affect the clinical validity and the image quality.

Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.).

Perilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of P. frutescens are limited due to a lack of gene annotations and characterization. A full-length cDNA library from P. frutescens leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags. Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO). A total of 18 simple sequence repeats (SSRs) were designed as primer pairs. This study is the first to report comparative genomics and EST-SSR markers from P. frutescens will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant.