Asunto(s)
Anacardium/química , Antiinfecciosos/química , Antineoplásicos/química , Cobre/química , Nanopartículas del Metal/química , Gomas de Plantas/química , Animales , Neoplasias de la Mama/tratamiento farmacológico , Línea Celular Tumoral , Supervivencia Celular , Sulfato de Cobre/química , Sulfato de Cobre/toxicidad , Eritrocitos/citología , Macrófagos/citología , Nanopartículas del Metal/toxicidad , Ratones , Ratones Endogámicos BALB C , Ovinos , Staphylococcus aureus/efectos de los fármacos , Propiedades de Superficie , Factores de TiempoRESUMEN
Proline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPP-BrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases.
Asunto(s)
Inhibidores de la Enzima Convertidora de Angiotensina/metabolismo , Anuros/metabolismo , Oligopéptidos/metabolismo , Piel/metabolismo , Secuencia de Aminoácidos , Inhibidores de la Enzima Convertidora de Angiotensina/síntesis química , Inhibidores de la Enzima Convertidora de Angiotensina/farmacología , Animales , Aorta Torácica/citología , Dominio Catalítico , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Células Endoteliales de la Vena Umbilical Humana , Humanos , Masculino , Óxido Nítrico/metabolismo , Oligopéptidos/química , Oligopéptidos/farmacología , Peptidil-Dipeptidasa A/química , Peptidil-Dipeptidasa A/metabolismo , Prolina/química , Unión Proteica , Estructura Secundaria de Proteína , Ratas , Ratas WistarRESUMEN
INTRODUCTION: Larva migrans visceral is caused by Toxocara sp and has never been studied in Bahia. This work investigated the prevalence and risk factors for infection by Toxocara canis in individuals from Salvador, State of Bahia. METHODS: Three hundred and thirty-eight individuals were investigated for the presence of serum IgG anti-T. canis. RESULTS: IgG anti-T. canis was higher in individuals from lower social classes who had more contact with dogs and cats, indicating that these variables are factors risk for this infection. CONCLUSIONS: The prevalence of T. canis infection was high. The risk factors for this infection identified are in agreement with in the literature.
Asunto(s)
Anticuerpos Antihelmínticos/sangre , Inmunoglobulina G/sangre , Toxocara canis/inmunología , Toxocariasis/epidemiología , Adolescente , Adulto , Animales , Brasil/epidemiología , Gatos , Perros , Femenino , Humanos , Masculino , Prevalencia , Factores de Riesgo , Factores Socioeconómicos , Toxocariasis/diagnóstico , Toxocariasis/transmisión , Adulto JovenRESUMEN
INTRODUÇÃO: Larva migrans visceral é causada por Toxocara sp e nunca foi estudada na Bahia. Neste trabalho, investigou-se a prevalência e fatores de risco de infecção por Toxocara canis, em indivíduos de Salvador. MÉTODOS: Trezentos e trinta e oito indivíduos foram investigados para presença de anticorpos IgG séricos anti-T. canis. RESULTADOS: IgG anti-T. canis foi mais alta em indivíduos de classe social baixa com maior contato com cães e gatos, indicando que estas variáveis são fatores de risco para esta infecção. CONCLUSÕES: A prevalência de infecção por T. canis foi alta. Os fatores de risco desta infecção encontrados estão de acordo com a literatura.
INTRODUCTION: Larva migrans visceral is caused by Toxocara sp and has never been studied in Bahia. This work investigated the prevalence and risk factors for infection by Toxocara canis in individuals from Salvador, State of Bahia. METHODS: Three hundred and thirty-eight individuals were investigated for the presence of serum IgG anti-T. canis. RESULTS: IgG anti-T. canis was higher in individuals from lower social classes who had more contact with dogs and cats, indicating that these variables are factors risk for this infection. CONCLUSIONS: The prevalence of T. canis infection was high. The risk factors for this infection identified are in agreement with in the literature.
