Experimental Research on Bonded Anchorage of Carbon Fiber Reinforced Polymer Prestressed Strands.

Aiming at the problems of a large number of corrosion and fatigue damage of the current prestressed steel strands, this paper adopts carbon fiber-reinforced composite (CFRP) strand with better corrosion resistance and fatigue resistance and uses it in concrete structures. The bond anchorage is usually used to anchor CFRP tension members, which bonds the CFRP through the binding medium. Through experimental research on the CFRP strand bond anchorage, the inner taper of the CFRP prestressed strand cone was anchored and the influence of different anchor lengths and bonding media on the anchorage performance was determined. The test results demonstrate that the taper of the conical anchorage described in this paper is a key factor affecting its anchorage performance and increasing the inner taper within a certain range is beneficial to improving the anchorage performance of the conical anchorage. The bonded anchorage of the CFRP prestressed strand with a 200 mm anchor is the most reliable and efficient, as the taper of the 200 mm anchor is the largest. The average anchoring efficiency coefficient of the 200 mm anchor was 96.4%, which is 3.7% and 2.6% higher than the average anchoring efficiency coefficient of 220 mm and 250 mm anchors, respectively. The anchoring efficiency of the anchor is also high (94.1%) when the epoxy resin mortar is used as the bonding medium. Moreover, after an appropriate amount of quartz sand is added to the epoxy resin, the overall comprehensive performance of the anchor can be improved to a certain extent and the stress of the CFRP strand can be improved. The coupling between ultra-high-performance concrete dry mix (UHPC-GJL) and CFRP strand materials is not suitable for UHPC-GJL being used, as its binding medium as the average anchoring efficiency coefficient is only 44.5% when UHPC-GJL is used as the anchor bonding medium.

Medical Sports Data Privacy Protection Method Based on Legal Risk Control.

With the continuous development of computer and network technology, the amount of information storage in medical information system is more and more large, which is prone to the problem of privacy information leakage, resulting in irreparable harm. In order to solve the problem of privacy leakage in the medical environment, a new privacy rating method is proposed according to the actual situation of the medical environment. The big data technology is used to effectively mine, analyze, integrate, and reuse medical data, and a new improved model is proposed. At the same time, the medical information system applying the improved model is designed according to the complex actual needs. The purpose of this paper is to correctly understand the positive role of medical sports big data (BD) research in the medical field and standardize the behavior of medical staff. On the one hand, it can improve the safety awareness of patients and enhance the standardization of medical treatment environment. This paper will analyze the meaning and research status of medical data from the perspective of legal risk control, focus on the status quo and existing problems of medical sports data privacy protection, and put forward positive countermeasures and some practical solutions. The results show that the medical sports information data has certain regularity and particularity, ease to spread, and mining. Hospitals and medical staff should make the areas and items restricted by law clear, standardize their own behaviors, constantly sum up experience, and actively improve and modify relevant measures.

Rice FLUORESCENT1 Is Involved in the Regulation of Chlorophyll.

Chlorophyll biosynthesis plays essential roles in photosynthesis and plant growth in response to environmental conditions. The accumulation of excess chlorophyll biosynthesis intermediates under light results in the production of reactive oxygen species and oxidative stress. In this study, we identified a rice (Oryza sativa) mutant, oxidation under photoperiod (oxp), that displayed photobleached lesions on its leaves, reduced growth and decreased chlorophyll content during light/dark cycles or following a dark-to-light transition. The oxp mutant accumulated more chlorophyll precursors (5-aminolevulinic acid and protochlorophyllide) than the wild type in the dark, and more singlet oxygen following light exposure. Several singlet-oxygen-responsive genes were greatly upregulated in oxp, whereas the expression patterns of OsPORA and OsPORB, two genes encoding the chlorophyll biosynthesis enzyme NADPH:protochlorop hyllide oxidoreductase, were altered in de-etiolated oxp seedlings. Molecular and complementation studies revealed that oxp is a loss-of-function mutant in LOC_Os01g32730, a homolog of FLUORESCENT (FLU) in Arabidopsis thaliana. Rice PHYTOCHROME-INTERACTING FACTOR-LIKE14 (OsPIL14) transcription factor directly bound to the OsFLU1 promoter and activated its expression. Dark-grown transgenic rice seedlings overexpressing OsPIL14 accumulated more chlorophyll and turned green faster than the wild type upon light illumination. Thus, OsFLU1 is an important regulator of chlorophyll biosynthesis in rice.

Evolution and expression analysis of the sorghum ubiquitin-conjugating enzyme family.

Ubiquitin-conjugating enzymes (UBCs), which catalyse the transfer of ubiquitin to substrate or E3 ligases, are key enzymes in ubiquitination modifications of target proteins. Current knowledge regarding the sorghum (Sorghum bicolor (L.) Moench) ubiquitin-conjugating enzyme (SbUBC) family remains very limited. We identified 53 UBC-encoding genes in the sorghum genome and divided these into 18 groups according to their phylogenetic relationship with Arabidopsis thaliana (L.) Heynh., which was further supported by conserved motif and gene structure analyses. Different expression levels under a variety of abiotic stresses suggested that these might participate in distinct signalling pathways and that they underwent functional divergence during evolution. Furthermore, several SbUBC genes responded to single treatments, and individual SbUBC genes responded to multiple treatments, suggesting that sorghum UBCs may mediate crosstalk among different signalling pathways. Overall, the results provide valuable information for better understanding the classification and putative functions of sorghum UBC-encoding genes.

The banana E2 gene family: Genomic identification, characterization, expression profiling analysis.

The E2 is at the center of a cascade of Ub1 transfers, and it links activation of the Ub1 by E1 to its eventual E3-catalyzed attachment to substrate. Although the genome-wide analysis of this family has been performed in some species, little is known about analysis of E2 genes in banana. In this study, 74 E2 genes of banana were identified and phylogenetically clustered into thirteen subgroups. The predicted banana E2 genes were distributed across all 11 chromosomes at different densities. Additionally, the E2 domain, gene structure and motif compositions were analyzed. The expression of all of the banana E2 genes was analyzed in the root, stem, leaf, flower organs, five stages of fruit development and under abiotic stresses. All of the banana E2 genes, with the exception of few genes in each group, were expressed in at least one of the organs and fruit developments, which indicated that the E2 genes might involve in various aspects of the physiological and developmental processes of the banana. Quantitative RT-PCR (qRT-PCR) analysis identified that 45 E2s under drought and 33 E2s under salt were induced. To the best of our knowledge, this report describes the first genome-wide analysis of the banana E2 gene family, and the results should provide valuable information for understanding the classification, cloning and putative functions of this family.

Genome-Wide Identification, Phylogenetic and Expression Analyses of the Ubiquitin-Conjugating Enzyme Gene Family in Maize.

BACKGROUND: Ubiquitination is a post-translation modification where ubiquitin is attached to a substrate. Ubiquitin-conjugating enzymes (E2s) play a major role in the ubiquitin transfer pathway, as well as a variety of functions in plant biological processes. To date, no genome-wide characterization of this gene family has been conducted in maize (Zea mays). METHODOLOGY/PRINCIPAL FINDINGS: In the present study, a total of 75 putative ZmUBC genes have been identified and located in the maize genome. Phylogenetic analysis revealed that ZmUBC proteins could be divided into 15 subfamilies, which include 13 ubiquitin-conjugating enzymes (ZmE2s) and two independent ubiquitin-conjugating enzyme variant (UEV) groups. The predicted ZmUBC genes were distributed across 10 chromosomes at different densities. In addition, analysis of exon-intron junctions and sequence motifs in each candidate gene has revealed high levels of conservation within and between phylogenetic groups. Tissue expression analysis indicated that most ZmUBC genes were expressed in at least one of the tissues, indicating that these are involved in various physiological and developmental processes in maize. Moreover, expression profile analyses of ZmUBC genes under different stress treatments (4°C, 20% PEG6000, and 200 mM NaCl) and various expression patterns indicated that these may play crucial roles in the response of plants to stress. CONCLUSIONS: Genome-wide identification, chromosome organization, gene structure, evolutionary and expression analyses of ZmUBC genes have facilitated in the characterization of this gene family, as well as determined its potential involvement in growth, development, and stress responses. This study provides valuable information for better understanding the classification and putative functions of the UBC-encoding genes of maize.

Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1?

Reactive oxygen species (ROS), like hydrogen peroxide (H2O2) and superoxide anion (O2(·-)), are important plant cell signaling molecules involved in diverse physiological processes, such as programmed cell death, development, cell elongation and hormonal signaling. Recently, much attention has been paid to the role of ROS in regulating plant root development. Two ROS, superoxide and hydrogen peroxide, were shown to exhibit a typical accumulation pattern in the Arabidopsis root apex and play distinct roles in root development. The latest study showed that UPBEAT1 (UPB1), a bHLH transcription factor, modulates the ROS balance by directly regulating the expression of a set of peroxidases, therefore, regulates the root cell proliferation and differentiation. In this addendum, we proposed a possible hypothesis that OsSPR1 maintained the mitochondria function to restrict H2O2 production in root apex for normal root development.

Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa.

• A rice mutant, Oryza sativa short postembryonic roots 1 (Osspr1), has been characterized. It has short postembryonic roots, including adventitious and lateral roots, and a lower iron content in its leaves. • OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain. • Osspr1 mutants exhibited decreased root cell elongation. The iron content of the mutant shoots was significantly altered compared with that of wild-type shoots. A similar pattern of alteration of manganese and zinc concentrations in shoots was also observed. Complementation of the mutant confirmed that OsSPR1 is involved in post-embryonic root elongation and iron homeostasis in rice. OsSPR1 was found to be ubiquitously expressed in various tissues throughout the plant. The transcript abundance of various genes involved in iron uptake and signaling via both strategies I and II was similar in roots of wild-type and mutant plants, but was higher in the leaves of mutant plants. • Thus, a novel mitochondrial protein that is involved in root elongation and plays a role in metal ion homeostasis has been identified.

OsCYT-INV1 for alkaline/neutral invertase is involved in root cell development and reproductivity in rice (Oryza sativa L.).

A short root mutant was isolated from an EMS-generated rice mutant library. Under normal growth conditions, the mutant exhibited short root, delayed flowering, and partial sterility. Some sections of the roots revealed that the cell length along the longitudinal axis was reduced and the cell shape in the root elongation zone shrank. Genetic analysis indicated that the short root phenotype was controlled by a recessive gene. Map-based cloning revealed that a nucleotide substitution causing an amino acid change from Gly to Arg occurred in the predicted rice gene (Os02g0550600). It coded an alkaline/neutral invertase and was homologous to Arabidopsis gene AtCyt-inv1. This gene was designated as OsCyt-inv1. The results of carbohydrate analysis showed an accumulation of sucrose and reduction of hexose in the Oscyt-inv1 mutant. Exogenously supplying glucose could rescue the root growth defects of the Oscyt-inv1 mutant. These results indicated that OsCyt-inv1 played important roles in root cell development and reproductivity in rice.